Architecture of interstitial nodal spaces in the rodent renal inner medulla

Gilbert, Rebecca L.; Pannabecker, Thomas L.

doi:10.1152/ajprenal.00239.2013

Cited by 5 publications

(4 citation statements)

References 29 publications

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“…Proposed to serve as mixing compartments for NaCl, urea, and water reabsorbate (21), these interstitial nodal spaces are abundant in the rodent outer inner medulla and decline significantly in number with increasing depth below the outer medulla (13,36). If these compartments do play a role in rodent renal physiological processes, then the absence of this architectural feature in the human inner medulla could be related to substantive differences between human and rodent physiology.…”

Section: Discussionmentioning

confidence: 99%

Architecture of the human renal inner medulla and functional implications

Wei

Rosen

Dantzler

et al. 2015

American Journal of Physiology-Renal Physiology

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The architecture of the inner stripe of the outer medulla of the human kidney has long been known to exhibit distinctive configurations; however, inner medullary architecture remains poorly defined. Using immunohistochemistry with segment-specific antibodies for membrane fluid and solute transporters and other proteins, we identified a number of distinctive functional features of human inner medulla. In the outer inner medulla, aquaporin-1 (AQP1)-positive long-loop descending thin limbs (DTLs) lie alongside descending and ascending vasa recta (DVR, AVR) within vascular bundles. These vascular bundles are continuations of outer medullary vascular bundles. Bundles containing DTLs and vasa recta lie at the margins of coalescing collecting duct (CD) clusters, thereby forming two regions, the vascular bundle region and the CD cluster region. Although AQP1 and urea transporter UT-B are abundantly expressed in long-loop DTLs and DVR, respectively, their expression declines with depth below the outer medulla. Transcellular water and urea fluxes likely decline in these segments at progressively deeper levels. Smooth muscle myosin heavy chain protein is also expressed in DVR of the inner stripe and the upper inner medulla, but is sparsely expressed at deeper inner medullary levels. In rodent inner medulla, fenestrated capillaries abut CDs along their entire length, paralleling ascending thin limbs (ATLs), forming distinct compartments (interstitial nodal spaces; INSs); however, in humans this architecture rarely occurs. Thus INSs are relatively infrequent in the human inner medulla, unlike in the rodent where they are abundant. UT-B is expressed within the papillary epithelium of the lower inner medulla, indicating a transcellular pathway for urea across this epithelium.

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Section: Discussionmentioning

confidence: 99%

Architecture of the human renal inner medulla and functional implications

Wei

Rosen

Dantzler

et al. 2015

American Journal of Physiology-Renal Physiology

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“…Outer medullary interbundle regions are occupied by collecting ducts (CDs), thick ascending limbs, descending thin limbs of long-loop nephrons, and networks of fenestrated interconnecting capillaries (ascending vasa recta) (2, 27). The inner medullary intracluster regions are occupied by collecting ducts, networks of fenestrated interconnecting capillaries (ascending vasa recta), ascending thin limbs, and to a lesser extent aquaporin-1-null descending thin limbs(19,55,74). Unbranched ascending vasa recta residing in the medullary interbundle and intracluster regions(27) are not shown in B.…”

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confidence: 99%

Targeted delivery of solutes and oxygen in the renal medulla: role of microvessel architecture

Pannabecker

2014

American Journal of Physiology-Renal Physiology

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Renal medullary function is characterized by corticopapillary concentration gradients of various molecules. One example is the generally decreasing axial gradient in oxygen tension (Po2). Another example, found in animals in the antidiuretic state, is a generally increasing axial solute gradient, consisting mostly of NaCl and urea. This osmolality gradient, which plays a principal role in the urine concentrating mechanism, is generally considered to involve countercurrent multiplication and countercurrent exchange, although the underlying mechanism is not fully understood. Radial oxygen and solute gradients in the transverse dimension of the medullary parenchyma have been hypothesized to occur, although strong experimental evidence in support of these gradients remains lacking. This review considers anatomic features of the renal medulla that may impact the formation and maintenance of oxygen and solute gradients. A better understanding of medullary architecture is essential for more clearly defining the compartment-to-compartment flows taken by fluid and molecules that are important in producing axial and radial gradients. Preferential interactions between nephron and vascular segments provide clues as to how tubular and interstitial oxygen flows contribute to safeguarding active transport pathways in renal function in health and disease.

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“…Furthermore, the oxygen consuming tubular elements in the outer medulla, the thick ascending limbs of the loop of Henle (mTALH) and the collecting ducts (CD), are situated chiefly in the inter‐bundle region, at some distance from the long descending vasa recta . In the inner medulla of the rat, each CD is surrounded by closely associated ascending vasa recta, with clusters of descending vasa recta more distant to the CD . In a series of modelling studies of increasing complexity, Layton and Edwards and their colleagues examined the impact of this topography on oxygenation of the outer and inner medulla (multiple publications discussed below).…”

Section: Renal Oxygenation: What Are the Critical Questions Mathematimentioning

confidence: 99%

“…55,56 In the inner medulla of the rat, each CD is surrounded by closely associated ascending vasa recta, with clusters of descending vasa recta more distant to the CD. 57,58 In a series of modelling studies of increasing complexity, Layton and Edwards and their colleagues examined the impact of this topography on oxygenation of the outer and inner medulla (multiple publications discussed below). In addition, using a sophisticated multiscale porous media approach to modelling renal oxygenation, Lee et al modelled oxygen transport in a three-dimensional rat renal medulla.…”

Section: What Makes the Renal Medulla Susceptible To Hypoxia?mentioning

confidence: 99%

Renal oxygenation: From data to insight

et al. 2020

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Computational models have made a major contribution to the field of physiology. As the complexity of our understanding of biological systems expands, the need for computational methods only increases. But collaboration between experimental physiologists and computational modellers (ie theoretical physiologists) is not easy. One of the major challenges is to break down the barriers created by differences in vocabulary and approach between the two disciplines. In this review, we have two major aims. Firstly, we wish to contribute to the effort to break down these barriers and so encourage more interdisciplinary collaboration. So, we begin with a “primer” on the ways in which computational models can help us understand physiology and pathophysiology. Second, we aim to provide an update of recent efforts in one specific area of physiology, renal oxygenation. This work is shedding new light on the causes and consequences of renal hypoxia. But as importantly, computational modelling is providing direction for experimental physiologists working in the field of renal oxygenation by: (a) generating new hypotheses that can be tested in experimental studies, (b) allowing experiments that are technically unfeasible to be simulated in silico, or variables that cannot be measured experimentally to be estimated, and (c) providing a means by which the quality of experimental data can be assessed. Critically, based on our experience, we strongly believe that experimental and theoretical physiology should not be seen as separate exercises. Rather, they should be integrated to permit an iterative process between modelling and experimentation.

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Architecture of interstitial nodal spaces in the rodent renal inner medulla

Cited by 5 publications

References 29 publications

Architecture of the human renal inner medulla and functional implications

Architecture of the human renal inner medulla and functional implications

Targeted delivery of solutes and oxygen in the renal medulla: role of microvessel architecture

Renal oxygenation: From data to insight

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