Architecture of the cortical actomyosin network driving apical constriction in C. elegans

Abstract

Apical constriction is a cell shape change that drives key morphogenetic events during development, including gastrulation and neural tube formation. The forces driving apical constriction are primarily generated through the contraction of apicolateral and/or medioapical actomyosin networks. In the Drosophila ventral furrow, the medioapical actomyosin network has a sarcomere-like architecture, with radially polarized actin filaments and centrally enriched non-muscle myosin II and myosin activating kinase. To determine if this is a broadly conserved actin architecture driving apical constriction, we examined actomyosin architecture during C. elegans gastrulation, in which two endodermal precursor cells internalize from the surface of the embryo. Quantification of protein localization showed that neither the non-muscle myosin II NMY-2 nor the myosin-activating kinase MRCK-1 is enriched at the center of the apex. Further, visualization of barbed- and pointed-end capping proteins revealed that actin filaments do not exhibit radial polarization at the apex. Our results demonstrate that C. elegans endodermal precursor cells apically constrict using a mixed-polarity actin filament network and with myosin and a myosin activator distributed throughout the network. Taken together with observations made in other organisms, our results demonstrate that diverse actomyosin architectures are used in animal cells to accomplish apical constriction.

Classification of human actin pathological variants usingC. elegansCRISPR-generated models

“…First, at the zygotic stage due to a failure to complete cell division (orange in Figure 3F). Second, developmental arrest during gastrulation (yellow in Figure 3F), a key event involving cell shape changes and migration, highly dependent on Arp2/3 actin networks (Nance 2005;Zhang, Medwig-Kinney, and Goldstein 2023). Third, in the vast majority, developmental arrest occurred at later steps of development.…”