Architecture of the Spermatozoid of Selaginella australiensis

Renzaglia, Karen S.; Dengate, Sandra B.; Bernhard, Douglas L.

doi:10.2307/1547309

Cited by 9 publications

(6 citation statements)

References 16 publications

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“…Microsporangia were processed for TEM observation according to the technique outlined in Renzaglia et al (1998). Microsporangia were placed in 6% glutaraldehyde in 0.05 M Pipes buffer (pH 7.4), and microspores were liberated and gently cracked with the end of a flame-sealed Pasteur pipette.…”

Section: Methodsmentioning

confidence: 99%

“…In cellular architecture, the spermatozoid of Selaginella bears a striking resemblance to those of mosses and liverworts (Robert 1974;Garbary et al 1993;Renzaglia et al 1998). Common spermatozoid features among these plants include the streamlined coiled morphology, the arrangement and number of organelles, and the staggered insertion of the two rearwardly directed, apical flagella.…”

Section: Introductionmentioning

confidence: 99%

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Developmental Ultrastructure of the Male Gamete ofSelaginella

Renzaglia¹,

Bernhard²,

Garbary³

1999

International Journal of Plant Sciences

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JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact support@jstor.org.. The University of Chicago Press is collaborating with JSTOR to digitize, preserve and extend access to International Journal of Plant Sciences.To evaluate structural homology among streamlined, biflagellated male gametes of Selaginella and bryophytes, we undertook a thorough ultrastructural study of spermatogenesis in Selaginella kraussiana (Kunze) A. Br. As in Lycopodium and bryophytes, centrioles originate as bicentrioles in the spermatid mother cell. In the nascent spermatid, a fibrous connective band is involved in the separation and rotation of the two centriolar constituents of the bicentriole. The locomotory apparatus consists of two staggered monomorphic basal bodies that originate from the centrioles and an underlying multilayered structure (MLS), including a spline of parallel microtubules and subtending elongated lamellar strip. Beneath the MLS lies the anterior mitochondrion, which elongates during development to a length of over 1.5 cellular revolutions by progressive fusion of mitochondria. Spline microtubules (MTs) increase posteriorly from one at the anterior tip to a maximum of 19 at the cell posterior. The angle between lamellar strip plates and spline MTs changes front to back from 85Њ to 40Њ. In the late midstage spermatid, the rounded nucleus compacts and elongates in register with spline growth. Individualization of cellular coils begins at the cell anterior and progresses posteriorly leaving a cytoplasmic mass in the region between coils. In the final stages of development, the lamellar strip regresses and the cylindrical nucleus shortens. Concomitantly, the posterior mitochondrion and plastid, containing two starch grains, are positioned alongside the spline at the posterior of the cell. In development, the spermatozoid of Selaginella more closely resembles those of pteridophytes than bryophytes. Common developmental and structural features of the locomotory apparatus among Selaginella and members of the Lycopodiaceae are consistent with a monophyletic lycopsid assemblage.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Developmental Ultrastructure of the Male Gamete ofSelaginella

Renzaglia¹,

Bernhard²,

Garbary³

1999

International Journal of Plant Sciences

Self Cite

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“…This developmental phenomenon is evidenced by a stellate pattern in the mature cell that is internal and external to the cell limit. Having been observed in other Lycopodiaceae and to a lesser extent in Selaginella, this feature appears to be restricted to lycophyte spermatozoids (Maden et al, 1996(Maden et al, , 1997Renzaglia et al, 1998Renzaglia et al, , 1999. In ferns, cycads, and Ginkgo, the entire stellate pattern forms before basal bodies are fixed in position.…”

Section: Mtocs In Developing Sperm Cellsmentioning

confidence: 74%

Microtubule organizing centers and the origin of centrioles during spermatogenesis in the PteridophytePhylloglossum

Renzaglia

Maden

2000

Microsc. Res. Tech.

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“…Previous studies of spermatogenesis have largely ignored the importance of the EPM in growth of flagella and in cell morphogenesis (Duckett, 1973; Bell and Duckett, 1976; Renzaglia and Duckett, 1987; Renzaglia et al, 1998, 2001; Renzaglia and Garbary, 2001; Sakaushi et al, 2003; Lopez‐Smith and Renzaglia, 2008). Kotenko (1990) described a three‐layered “wall” around sperm in the fern Onoclea , but she did not stress the significance or components of any layer.…”

Section: Discussionmentioning

confidence: 99%

Multiflagellated sperm cells of Ceratopteris richardii are bathed in arabinogalactan proteins throughout development

Lopez

Renzaglia

2014

American J of Botany

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We demonstrated that AGPs are abundant in the EPM and along the plasmalemma and that the three AGP epitopes have specific expression patterns during development. Coupled with inhibition studies, these results identify AGPs as critical to the formation of an extraprotoplasmic matrix and the consequent origin and development of flagella in an orderly and precise fashion around the cell. We speculate that AGPs may play additional roles as signaling molecules involved in cell shaping, cytoskeletal development, vesicle trafficking, and cytoplasmic elimination.

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Architecture of the Spermatozoid of Selaginella australiensis

Cited by 9 publications

References 16 publications

Developmental Ultrastructure of the Male Gamete ofSelaginella

Developmental Ultrastructure of the Male Gamete ofSelaginella

Microtubule organizing centers and the origin of centrioles during spermatogenesis in the PteridophytePhylloglossum

Multiflagellated sperm cells of Ceratopteris richardii are bathed in arabinogalactan proteins throughout development

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