Arcobacter haliotis sp. nov., isolated from abalone species Haliotis gigantea

Abstract

A Gram-negative, aerobic, polar-flagellated and rod-shaped, sometimes slightly curved bacterium, designated MA5T, was isolated from the gut of an abalone of the species Haliotis gigantea collected in Japan. Phylogenetic analyses based on 16S rRNA, gyrB, hsp60 and rpoB gene sequences placed strain MA5T in the genus Arcobacter in an independent phylogenetic line. Comparison of the 16S rRNA gene sequence of this strain with those of the type strains of the established Arcobacter species revealed A. nitrofigilis (95.1 %) as nearest neighbour. Strain MA5T grew optimally at 25 °C, pH 6.0 to 9.0 and in the presence of 2 to 5 % (w/v) NaCl under both aerobic and microaerobic conditions. The predominant fatty acids found were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c), C12 : 0 3-OH and C18 : 1 ω7c. Menaquinone-6 (MK-6) and menaquinone-7 (MK-7) were found as the major respiratory quinones. The major polar lipids detected were phosphatidylethanolamine and phosphatidylglycerol. Strain MA5T could be differentiated phenotypically from the phylogenetic closest Arcobacter species by its ability to grow on 0.05 % safranin and 0.01 % 2,3,5-triphenyl tetrazolium chloride (TTC), but not on 0.5 % NaCl. The obtained DNA G+C content of strain MA5T was 27.9 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic distinctiveness of MA5T, this strain is considered to represent a novel species of the genus Arcobacter, for which the name Arcobacter haliotis sp. nov. is proposed. The type strain is MA5T (=LMG 28652T=JCM 31147T).

Revisiting the Taxonomy of the Genus Arcobacter: Getting Order From the Chaos

“…However, this species showed atypical characteristics, including lack of motility and obligate anaerobic metabolism, which led to the original description of the genus Arcobacter being emended ( Sasi-Jyothsna et al, 2013 ). The most recently described species from shellfish are A. lekithochrous , A. haliotis , and A. canalis ( Diéguez et al, 2017 ; Tanaka et al, 2017 ; Pérez-Cataluña et al, 2018a ). The first one included several isolates recovered from scallop larvae and from tank seawater of a Norwegian hatchery ( Diéguez et al, 2017 ), the second species came from an abalone of Japan ( Tanaka et al, 2017 ) and the third from oysters submerged in a water channel contaminated with wastewater ( Pérez-Cataluña et al, 2018a ).…”

“…The most recently described species from shellfish are A. lekithochrous , A. haliotis , and A. canalis ( Diéguez et al, 2017 ; Tanaka et al, 2017 ; Pérez-Cataluña et al, 2018a ). The first one included several isolates recovered from scallop larvae and from tank seawater of a Norwegian hatchery ( Diéguez et al, 2017 ), the second species came from an abalone of Japan ( Tanaka et al, 2017 ) and the third from oysters submerged in a water channel contaminated with wastewater ( Pérez-Cataluña et al, 2018a ). However, Diéguez et al (2018) evidenced that the species A. haliotis is a later heterotypic synonym of A. lekithochrous .…”

Dynamics of the Pacific oyster pathobiota during mortality episodes in Europe assessed by 16S rRNA gene profiling and a new target enrichment next‐generation sequencing strategy

“…represent an important fraction of the oyster microbiota whose relative abundance significantly increased in concomitance with V. aestuarianus infection. The species Arcobacter haliotis was isolated from the gut of an abalone of the species Haliotis gigantea collected in Japan (Tanaka et al, 2017), however, taxonomy of the Arcobacter sp. strain found in this study and its potential role as an opportunistic pathogen for C. gigas deserve further investigations (Pérez-Cataluña et al, 2018).…”

A Polyphasic and Taxogenomic Evaluation Uncovers Arcobacter cryaerophilus as a Species Complex That Embraces Four Genomovars

“…In addition, they created a new family Arcobacteraceae that includes only the genus Arcobacter . Currently, the genus Arcobacter includes 27 species (Park et al, 2016 ; Whiteduck-Léveillée et al, 2016 ; Diéguez et al, 2017 ; Figueras et al, 2017 ; Tanaka et al, 2017 ; Pérez-Cataluña et al, 2018 ), four of which have been linked with human disease: Arcobacter butzleri, A. cryaerophilus, A. thereius , and A. skirrowii (Collado and Figueras, 2011 ; Figueras et al, 2014 ; Ferreira et al, 2015 ). The species A. cryaerophilus has been found in many food products of animal origin (like poultry, pork, lamb, and seafood and in dairy food processing facilities (Collado et al, 2008 ; Collado and Figueras, 2011 ).…”