Arctigenin Attenuates Lipopolysaccharide-Induced Acute Lung Injury in Rats

Abstract

Arctigenin (ATG) has been reported to possess anti-inflammatory properties. However, the effects of ATG on lipopolysaccharide (LPS)-induced acute lung injury (ALI) remains not well understood. In the present study, our investigation was designed to reveal the effect of ATG on LPS-induced ALI in rats. We found that ATG pretreatment attenuated the LPS-induced ALI, as evidenced by the reduced histological scores, myeloperoxidase activity, and wet-to-dry weight ratio in the lung tissues. This was accompanied by the decreased levels of tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), and interleukin-1 (IL-6) in the bronchoalveolar lavage fluid. Furthermore, ATG downregulated the expression of nuclear factor kappa B (NF-κB) p65, promoted the phosphorylation of inhibitor of nuclear factor-κB-α (IκBα) and activated the adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPKα) in the lung tissues. Our results suggested that ATG attenuates the LPS-induced ALI via activation of AMPK and suppression of NF-κB signaling pathway.

Lactoferrin suppresses lipopolysaccharide-induced endometritis in mice via down-regulation of the NF-κB pathway

“…The NF-κB signaling pathway plays an important role in the regulation of the inflammatory and immune responses, inducing the transcription of a series of genes encoding cytokines and chemokines [32]. Under normal conditions, NF-κB is associated with its inhibitor IκB-α in the cytoplasm [33]. LPS is an important activator of NF-κB signaling pathway.…”

Arctigenin prevents the progression of osteoarthritis by targeting PI3K/Akt/NF‐κB axis: In vitro and in vivo studies

“…Mice in the DMM group received a gavage of vehicle (0.5% CMC‐Na, a dietary cosolvent), while mice in the DMM + ATG group received oral administration of ATG (30 mg/kg, dissolved in CMC‐Na) daily for 8 weeks. This dose of ATG was prepared by adapting the procedure used in the previous study and our preliminary experiments. The mice were maintained in a temperature‐controlled room (20 ± 2°C) on a 12:12‐h light‐dark cycle and given free access to water and food.…”

“…The anti‐inflammatory effect has been confirmed on LPS‐induced inflammation models in RAW264.7 cells or human U937 macrophage cells by means of restraining NF‐κB, JAK‐STAT and MAPK pathway . Additionally, a recent study has exposited that ATG remits LPS‐induced acute lung injury in rats . To the most important, the ATG was found to exhibit the ability to protect against rheumatoid arthritis by target Akt/NF‐κB .…”

Novel regulators of endothelial barrier function

“…AMPK, a serine threonine kinase, regulates metabolic as well as anti-inflammatory functions (106,126). AMPK was shown to suppress TLR4-dependent activation of nuclear factor NF-B and the subsequent generation of cytokines (121,137) and facilitated phagocytosis and clearance of apoptotic cells (107,162), indicating that AMPK contributes to the resolution of inflammation.…”