Arctigenin enhances swimming endurance of sedentary rats partially by regulation of antioxidant pathways

Wu, Rui; Sun, Yue; Zhou, Tingting; Zhu, Zhiyuan; Zhuang, Jingjing; Tang, Xuan; Chen, Jing; Hu, Lihong; Shen, Xu

doi:10.1038/aps.2014.70

Cited by 57 publications

(29 citation statements)

References 63 publications

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“…XO is an enzyme that catalyzes the oxidation of hypoxanthine to xanthine and could generate reactive oxygen species, leading to oxidative stress (Ardan et al, 2004). On the other hand, the p53, a tumor-suppressor protein, exhibits antioxidant activities to eliminate oxidative stress and ensure cell survival in response to low levels of oxidative stresses through the p53-regulated gene GPX1, manganese superoxide dismutase (MNSOD) or other pathways (Sablina et al, 2005;An et al, 2008;Liu and Xu, 2011;Chen et al, 2012;Wu et al, 2014). As we expected, the transcript level of XO in mice treated with B10 being obviously down-regulated compared with HFD group, p53 expression is markedly up-regulated (Fig.…”

Section: Discussionmentioning

confidence: 99%

Effect of dietary supplementation of Bacillus subtilis B10 on biochemical and molecular parameters in the serum and liver of high-fat diet-induced obese mice

Lou

Wang

et al. 2015

J. Zhejiang Univ. Sci. B

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While a high-fat diet (HFD) is assumed to be related to fat-mediated oxidative stress decreasing antioxidant enzyme activity, probiotics are believed to have positive effects on the regulation of HFD-induced obesity as well as lipid metabolism, energy homeostasis, and anti-oxidation. Because Bacillus subtilis B10 has beneficial effects on the abnormal lipid metabolism and the oxidative stress in HFD-induced obese mice, ICR mice were randomly assigned into an HFD group and the HFD was supplemented with 0.1% (w/w) Bacillus subtilis B10 (HFD+B10 group). Thereafter, 30-d treatments were run, and then hepatic lipid level and antioxidant status were measured. The expression of genes related to lipid metabolism and oxidative stress in the liver was determined by reverse-transcription quantitative polymerase chain reaction (RT-qPCR). We found that HFD-induced obese mice treated with B10 showed a decrease in weight gain, serum glucose activity as well as hepatic triglyceride (TG), glutamic oxaloacetic transaminase (GOT), and glutamic pyruvic transaminase (GPT) activities. In addition, the gene expressions of antioxidant genes, glutathione reductase (GR), xanthine oxidase (XO), heat-shock protein 90 (Hsp90), and lipid synthesis gene 3β-hydroxysteroid-∆24 reductase (DHCR24) in the HFD+B10 group were down-regulated, suggesting alleviation of oxidative stress, while the lipolysis gene 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2), energy metabolism gene peroxisome proliferator-activated receptor α (PPARα) and the gene encoding tumor-suppressor protein p53 were up-regulated. The regulatory and positive effect of dietary supplementation of probiotic B10 suggests that it has a beneficial effect on the homeostasis of the lipid metabolism and on alleviating oxidative stress in HFD-induced obese mice.

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Section: Discussionmentioning

confidence: 99%

Effect of dietary supplementation of Bacillus subtilis B10 on biochemical and molecular parameters in the serum and liver of high-fat diet-induced obese mice

Lou

Wang

et al. 2015

J. Zhejiang Univ. Sci. B

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“…As reported previously, hypoxia is also associated with energy metabolism (11). 5'-AMP-activated protein kinase (AMPK) is a key regulator of cellular and whole body energy balance (12). It acts to suppress anabolic ATP-consumption pathways, and stimulates catabolic ATP-generating pathways (13).…”

Section: Introductionmentioning

confidence: 88%

Investigations on the antifatigue and antihypoxic effects of Paecilomyces hepiali extract

Wang

Liu

et al. 2015

Molecular Medicine Reports

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Abstract. Paecilomyces hepiali, one of the most valuable and effective Chinese medicinal herbs, possesses potential antioxidant, immunomodulatory, antitumor and anti-inflammatory properties. The present study aimed to investigate the antifatigue and antihypoxic effects of Paecilomyces hepiali extract (PHC) in a mouse model. Using a rotating rod, forced swimming and running assessment, the antifatigue activity of PHC was determined. PHC administration for 7 days had no effect on mouse horizontal or vertical movement, indicating no neurotoxicity at the selected doses was observed. Using a normobaric hypoxia, sodium nitrite toxicosis and acute cerebral ischemia assessments, PHC was confirmed to possess antihypoxic effects. PHC treatment for 7 days significantly enhanced the serum and liver levels of adenosine triphosphate, superoxide dismutase and glutathione peroxidase, prior to and following 60 min of swimming. The levels of antioxidant-associated proteins in the livers of the mice were analyzed using western blotting. PHC effectively increased the expression levels of phosphorylated (p)-5'-monophosphate (AMP)-activated protein kinase (AMPK), p-protein kinase B (AKT) and p-mammalian target of rapamycin (mTOR). The results of the present study demonstrated that PHC efficiently enhanced endurance from fatigue and had antihypoxic effects through elevation of the antioxidant capacity in the serum and liver, at least in part through the AMPK and AKT/mTOR pathways. These results indicate the potential of this natural product as an antioxidant in the treatment of fatigue, hypoxia and their associated diseases.

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“…Due to their chemical structure, phytoestrogens can be classed as isoflavones and lignins. Arctigenin (Ar, Figure 1) is a lignin phytoestrogen extracted from Arctium lappa (Gao, Li, Zhang, & Zeng, 2008) that displays anti-inflammatory (Gao, Yang, & Zuo, 2018), antioxidant (Wu et al, 2014), antifibrosis (Lin et al, 2019), and antitumor effects (Maxwell, Chun, Lee, Kim, & Nam, 2017). In preliminary experiments, we found that arctigenin promotes the differentiation of bone marrow mesenchymal stem cells (BMSCs) of ovariectomized (OVX) rats into osteoblasts, leading to anti-osteoporosis effects.…”

Section: Introductionmentioning

confidence: 99%

Arctigenin promotes bone formation involving PI3K/Akt/PPARγ signaling pathway

Chen

Lan

et al. 2020

Chem Biol Drug Des

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This study investigated the mechanisms through which arctigenin promotes osteogenesis. Bone marrow mesenchymal stem cells (BMSCs) from ovariectomized (OVX) rats were differentiated into osteoblasts, and osteogenesis was evaluated via Alizarin Red S (ARS) staining and alkaline phosphatase (ALP) measurements in cultured BMSCs. The levels of phosphorylated AKT serine/threonine kinase 1 (p‐Akt), and peroxisome proliferator‐activated receptor gamma (PPARγ) expression were quantified by Western blot analysis. The levels of urine calcium (U‐Ca), urine phosphorus (U‐P), serum ALP, and bone mineral density (BMD) of OVX rats were assessed in vivo. The results showed that treatment with arctigenin in rat BMSCs enhanced mineralization, increased ALP activity, increased the expression of Akt and p‐Akt, and decreased PPARγ expression, consistent with its ability to promote osteoblast differentiation. Furthermore, arctigenin prevented OVX‐induced osteoporosis in rats by increasing BMD and ALP activity and inhibiting the loss of Ca and P. In contrast, treatment with LY294002, a selective inhibitor of the phosphatidylinositol 3‐kinase (PI3K), produced the opposite phenotype. These data suggest that the protective effects of arctigenin on BMSCs and OVX rat models result from the induction of osteogenesis involving the PI3K/Akt/PPARγ axis.

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Arctigenin enhances swimming endurance of sedentary rats partially by regulation of antioxidant pathways

Abstract: Arctigenin efficiently enhances rat swimming endurance by elevation of the antioxidant capacity of the skeletal muscles, which has thereby highlighted the potential of this natural product as an antioxidant in the treatment of fatigue and related diseases.

Cited by 57 publications

References 63 publications

Effect of dietary supplementation of Bacillus subtilis B10 on biochemical and molecular parameters in the serum and liver of high-fat diet-induced obese mice

Effect of dietary supplementation of Bacillus subtilis B10 on biochemical and molecular parameters in the serum and liver of high-fat diet-induced obese mice

Investigations on the antifatigue and antihypoxic effects of Paecilomyces hepiali extract

Arctigenin promotes bone formation involving PI3K/Akt/PPARγ signaling pathway

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