Are Brazilian cervids at risk of prion diseases?

Falcão, Caio Bruno Ribeiro; Lima, Isabel Luiza de Melo Nunes Freire; Duarte, José Maurício Barbanti; Oliveira, João Ricardo Mendes de; Torres, Rodrigo A.; Wanderley, Artur Maia; Cunha, José Eriton Gomes da; García, José Eduardo

doi:10.1080/19336896.2016.1274000

Cited by 5 publications

(6 citation statements)

References 25 publications

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“…However, the susceptibility of such species to the majority of livestock pathogens is poorly understood. Health studies in South American deer species have mainly focused on serological surveys, e.g., brown brocket deer (BBD; Mazama gouazoubira ) in Bolivia [ 19 ], Argentinean Pampas deer ( Ozotoceros bezoarticus ) [ 20 ], and several studies of free-ranging and captive Brazilian deer populations [ 21 – 26 ].…”

Section: Introductionmentioning

confidence: 99%

A retrospective pathology study of two Neotropical deer species (1995-2015), Brazil: Marsh deer (Blastocerus dichotomus) and brown brocket deer (Mazama gouazoubira)

et al. 2018

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This retrospective study describes the biological and epidemiological aspects, gross and microscopical findings, and most likely causes of death (CD) in two species of Neotropical deer in Brazil. The animals were collected between 1995 and 2015 and represented 75 marsh deer (MD) and 136 brown brocket deer (BBD). Summarized, pneumonia was diagnosed microscopically in 48 MD and 52 BBD; 76 deer suffered trauma, involving dog attack (14 BBD) and vehicle-collision (14 BBD). Pulmonary edema (50 MD; 55 BBD) and congestion (57 MD; 78 BBD) were the most common findings for both species. Additionally, we diagnosed ruminal and myocardial mycosis in MD and BBD, respectively; ovarian dysgerminoma and pancreatic trematodiasis in BBD; and lesions suggestive of malignant catarrhal fever and orbiviral hemorrhagic disease in both species. The main CD in MD was: respiratory (41/75), alimentary, nutritional, trauma and euthanasia (3/75 each). Correspondingly, in BBD were: trauma (34/131), respiratory (30/131) and euthanasia (9/131). Respiratory disease was often defined by pulmonary edema and pneumonia. We provide evidence that respiratory disease, mainly pneumonia, is a critical pathological process in these Neotropical deer species. Although no etiological agents were identified, there is evidence of bacterial and viral involvement. Our results show trauma, mainly anthropogenic, as a common ailment in BBD. We propose to prioritize respiratory disease in future research focused on South American deer health aspects. We believe anthropogenic trauma may be a primary threat for populations of BBD.

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Section: Introductionmentioning

confidence: 99%

A retrospective pathology study of two Neotropical deer species (1995-2015), Brazil: Marsh deer (Blastocerus dichotomus) and brown brocket deer (Mazama gouazoubira)

et al. 2018

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“…In addition, the 23 and 12 bp insertion/deletion polymorphisms located in the promoter region were associated with the expression of the bovine PRNP gene and were reported to affect vulnerability to BSE ( 12 ). Furthermore, genetic polymorphisms at codons 95, 96, and 132 of the cervid PRNP gene played an important role in susceptibility to CWD in the cervidae family ( 13 – 15 ). Recent studies have reported that prion disease-resistant specific amino acids of PrPs, including S167 in horses and D159 in dogs, conferred resistance to conversion from PrP C to PrP Sc ( 16 , 17 ).…”

Section: Introductionmentioning

confidence: 99%

Novel Single Nucleotide Polymorphisms (SNPs) and Genetic Features of the Prion Protein Gene (PRNP) in Quail (Coturnix japonica)

Kim

Jeong

2022

Front. Vet. Sci.

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Prion diseases are fatal infectious diseases caused by conformational changes of a prion protein (PrPSc) derived from a normal prion protein (PrPC). Prion diseases have been reported in several mammalian hosts but not in any birds, including the most popular poultry species, of which chickens showed some resistance to experimental prion infection. To identify the genetic polymorphisms in the quail prion protein gene (PRNP), polymerase chain reaction and DNA sequencing were performed with gene-specific primers in 164 quails. Four in silico programs, including PROVEAN, PANTHER, SIFT, and AMYCO, were used to investigate the effect of non-synonymous single nucleotide polymorphisms (SNPs) on quail PrP. Furthermore, to investigate the genetic relationship of avian PrPs, phylogenetic analysis and multiple sequence alignments were performed using MEGA X program. Finally, the secondary and tertiary structures of avian PrPs were analyzed by SWISS-MODEL. We identified 33 novel SNPs in the quail PRNP gene, including three non-synonymous SNPs, c.56C>T (T19I), c.60C>T (V21I), and c.61G>A (A22S). Although V21I was predicted to have deleterious effects by SIFT, the substitutions of all three amino acids did not affect the amyloid propensity, 3D structure, or hydrogen bonds of quail PrP. Quail PrP showed a close evolutionary relationship and similar secondary and tertiary structures to chicken PrP compared to duck PrP. To our knowledge, this is the first report on the genetic and structural properties of the quail PRNP gene.

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“…Further, CWD has been detected in captive cervid populations in 18 U.S. states and three Canadian provinces (https://www.usgs.gov/centers/nwhc/science/expanding-distribution-chronic-wasting-disease). Current evidence (Falcão et al 2017; Mawdsley 2020; Buchholz et al 2021; Hannaoui et al 2017; Sohn et al 2002; Hamir et al 2011; Nalls et al 2013; Benestad et al 2016; Vikøren 2019; Cullingham et al 2020; USGS 2021) indicates that CWD occurs in several native North American cervid species, including white-tailed deer ( O. virginianus ), North American elk…”

Section: Introductionmentioning

confidence: 99%

A Non-invasive, Biomarker Assay for Detecting Chronic Wasting Disease Pathology in White-tailed Deer

Bradley

Roberts

Worsham

et al. 2022

Preprint

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We describe a non-invasive blood test that exploits differences in the abundance of diagnostic miRNA biomarkers associated with prion infections in cervids. Three potentially diagnostic and two control miRNAs were amplified in 93 serum samples from pen-raised white- tailed deer exposed to Chronic Wasting Disease (CWD). Initial analyses revealed sensitivity of the method to interference from hemolysis, requiring exclusion of excessively hemolyzed specimens from further analysis. Results of the miRNA test on the minimally hemolyzed samples from 33 animals were congruent (100% sensitivity, 92.3% specificity, 93.9% accuracy) with results from standard CWD diagnosis by immunohistochemical (IHC) detection of plaques in medial retropharyngeal lymph nodes and obex tissue, performed by a USDA certified laboratory. Overall, the miRNA assay performed as well as, if not superior to, other common CWD testing alternatives to IHC. Thus, this antemortem biomarker-based test offers a new addition to the resources available for monitoring and managing transmission and spread of CWD that is potentially more rapid and sensitive, and amenable to high throughput scaling.

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Are Brazilian cervids at risk of prion diseases?

Cited by 5 publications

References 25 publications

A retrospective pathology study of two Neotropical deer species (1995-2015), Brazil: Marsh deer (Blastocerus dichotomus) and brown brocket deer (Mazama gouazoubira)

A retrospective pathology study of two Neotropical deer species (1995-2015), Brazil: Marsh deer (Blastocerus dichotomus) and brown brocket deer (Mazama gouazoubira)

Novel Single Nucleotide Polymorphisms (SNPs) and Genetic Features of the Prion Protein Gene (PRNP) in Quail (Coturnix japonica)

A Non-invasive, Biomarker Assay for Detecting Chronic Wasting Disease Pathology in White-tailed Deer

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