Are changes in HSPA1A, HSPB1 and LDHb genes expression during physical performance ”till exhaustion” independent of their exercise possibility?

Abstract

Background: The aim of the study was to designate changes in the expression of HSPA1A, HSPB1 and LDHb in elite rowers after completing a test “till exhaustion” on a rowing ergometer. Finally, we searched for the answer whether there are significant correlations between the expression of the genes and anaerobic threshold (AnT) or the maximal oxygen uptake (VO2max).Material/Methods: The research was conducted on the sample of 9 Polish lightweight male rowers (23.7 ±3.77 yrs, 72.7 ±1.76 kg, 183.6 ±4.58 cm). To determine AnT and VO2max, the subjects performed the test “till exhaustion” with an increasing load on a rowing ergometer. Directly before and after the test, blood samples were collected from the ulnar vein in order to isolate genetic material. RNA was extracted from white cells of venous blood by the chemical method. 2 μg RNA for the reverse transcription was used and the expression of HSPA1A, HSPB1 and LDHb was determined by Real time PCR reaction. To assess the intensity of expression, the ΔΔCt method was used.Results: The study showed an increased expression of HSPA1A and HSPB1 and a decreased one of LDHb. Moreover, post-training changes of the genes activity in white blood cells occurred immediately and could be determined directly after the termination of exertion.Conclusions: No significant correlations between the expression of the genes and anaerobic threshold (AnT), maximal oxygen uptake (VO2max) were stated

Cellular Stress Response Gene Expression During Upper and Lower Body High Intensity Exercises

“…It is now clear that measuring changes in the expression of genes associated with the cellular stress response, such as HSPA1A and HSPB1 , synthesized by the cells of an organism in response to a variety of stimuli, including heat, oxidative, metabolic and chemical stress [ 9 , 10 ]may be useful in determining physical workload [ 11 ] and intensity [ 12 – 14 ]or to monitor training [ 15 ]. The literature also suggests that type of physical work [ 16 ] and type of training [ 17 ] may affect the expression of genes encoding heat shock protein (HSP) and interleukins. In addition, athletes showed a decreased expression of HSPA1A mRNA as an adaptation to exercise [ 18 ].…”

Assessment of fatigue and the overtraining syndrome in female spinning instructors based on a survey and on the expression of selected genes

“…Changes in the gene expression in peripheral blood leukocytes show a systemic response to effort or training and prove the stress load to the body [20,21,22]. The expression of numerous genes may increase locally in skeletal muscles, but there are differences between local and systemic changes in the mRNA levels.…”