OBJECTIVE: To compare Canadians of Aboriginal (First Nation, FN) and European ancestry (EA), with respect to obesity, subcutaneous fatness and relative subcutaneous fat distribution. DESIGN: Cross-sectional comparison. SUBJECTS: 118 First Nation and 472 European ancestry Canadians from Northern Ontario, youth (5 ± 19 y) and adults (20 ± 75 y). MEASUREMENTS: Stature, mass and skinfold thicknesses at the triceps, biceps, medial calf, subscapular, suprailiac and abdominal sites. ANALYSIS: Prevalence of obesity was determined using the 85 th percentile of body mass index (BMI) from NHANES II as the cut-off. Principal components (PC) analyses were performed on the six skinfolds. The ®rst component (PC1) represented a trunk-extremity skinfold contrast. Differences in mean component scores between FN and EA were assessed using independent samples t-tests. RESULTS: Prevalence of obesity among FN was high, ranging from 29% in youth to 60% in adult females. FN demonstrated greater subcutaneous adiposity and greater values for PC1, with the exception of adult males, where the difference is in the expected direction, but is not signi®cant. The direction of the differences indicates that the FN have a greater centralization of subcutaneous fat. CONCLUSIONS: FN Canadians generally have a greater prevalence of obesity, greater subcutaneous fatness and a more centralized distribution of body fat than those of European ancestry.