Are neutrophil extracellular traps the link for the cross-talk between periodontitis and rheumatoid arthritis physiopathology?

Abstract

Objectives Neutrophil extracellular traps (NETs) play a role in the pathogenesis of periodontitis and rheumatoid arthritis (RA). However, it remains poorly understood whether NETs participate in the cross-talk between periodontitis and RA. Herein, we investigated the production of NETs in individuals with periodontitis and RA and its association with clinical parameters. The impact of periodontal therapy on RA and NET release was also assessed. Methods The concentration of NETs and cytokines was determined in the saliva and plasma of individuals with early RA (n = 24), established RA (n = 64), and individuals without RA (n = 76). The influence of periodontitis on the production of NETs and cytokines was also evaluated. Results Individuals with early RA had a higher concentration of NETs in saliva and plasma than individuals with established RA or without RA. Periodontitis resulted in an increase in the concentration of NETs of groups of individuals without RA and with early RA. The proportion of individuals with high concentrations of IL-6, IL-10 and GM-CSF was higher among individuals with periodontitis than among individuals without periodontitis. The concentrations of TNF-α, IL-6, IL-17/IL-25, and IL-28A were particularly high in individuals with early RA. Worse periodontal clinical parameters, RA onset and RA activity were significantly associated with circulating NETs. Periodontal therapy was associated with a reduction in the concentration of NETs and inflammatory cytokines and amelioration in periodontitis and RA. Conclusion This study reveals that NETs are a possible link between periodontitis and RA, with periodontal therapy resulting in a dramatic switch in circulating NET levels.

Screening of crosstalk and pyroptosis-related genes linking periodontitis and osteoporosis based on bioinformatics and machine learning

“…In PD, NETs have been shown to accelerate the formation of periodontal pockets by promoting the inflammatory response (91) and may increase local soft and hard tissue destruction (92). Furthermore, a combined clinical study showed that periodontal treatment could lead to significant changes in circulating NETs, affecting clinical manifestations of other diseases (93). The formation of NETs was the most significant in the OP DEGs biological process in our study, suggesting that NETs also play an essential role in OP.…”

Receptor-Mediated NETosis on Neutrophils

“…Importantly, NETs play a role in the pathogenesis of periodontitis and RA ( 87 ). However, the mechanism of NET information in periodontitis is unclear.…”

The Citrullination-Neutrophil Extracellular Trap Axis in Chronic Diseases

“…In addition, there was a significant association in RA patients between the concentration of ACPAs and periodontal severity represented by plaque index, clinical attachment level, and number of pockets [ 85 ]. An important observation was made by Oliveira et al [ 86 ], who found an association between the concentration of circulating NETs, RA activity, and periodontal clinical parameters. Moreover, treatment of periodontal disease was significantly associated not only with the reduction of NET concentration but RA alleviation as well [ 86 ].…”

“…An important observation was made by Oliveira et al [ 86 ], who found an association between the concentration of circulating NETs, RA activity, and periodontal clinical parameters. Moreover, treatment of periodontal disease was significantly associated not only with the reduction of NET concentration but RA alleviation as well [ 86 ]. Besides ACPAs, anti-carbamylated protein antibodies were also significantly elevated in patients with RA and periodontitis compared to controls [ 87 ].…”