Are nitric oxide-mediated protein modifications of functional significance in diabetic heart? ye'S, -NO’, wh‘Y-NO’t?

Jayakumari, N.; Reghuvaran, Anand; Rajendran, Raji Sasikala; Pillai, Vivek; Karunakaran, Jayakumar; Sreelatha, Harikrishnan Vijayakumar; Gopala, Srinivas

doi:10.1016/j.niox.2014.08.002

Cited by 12 publications

(8 citation statements)

References 118 publications

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“…Increased eNOS might be a compensatory mechanism to endothelial damage in type 2 diabetes by an excess production of ROS related to ineffective NO action. 21 The enhanced NO production may contribute to hyperfiltration and microalbuminuria that characterizes early diabetic nephropathy. 22 The results of quantitative and qualitative expression patterns of the eNOS demonstrated that eNOS was virtually absent in control glomeruli but positive in some mesangial areas, glomeruli of diabetic nephropathy had an increased typical and prominent endothelial staining pattern.…”

Section: Discussionmentioning

confidence: 99%

The Puerarin improves renal function in STZ-induced diabetic rats by attenuating eNOS expression

Zhang

Wang

et al. 2015

Renal Failure

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Diabetic nephropathy (DN) is a serious complication and it leads to kidney failure. The endothelial nitric oxide synthases (eNOS) seems to be involved in the development and progression of DN. The Puerarin is a well-known Chinese traditional formula, which is widely used in clinical practice for the treatment of kidney disease. The present study was designed to investigate the renal protective effects of Puerarin on streptozotocin (STZ)-induced diabetic rats. Thirty Sprague-Dawley (SD) male rats were divided into three groups at random. The diabetic group and the Puerarin-treated group were intraperitoneally injected with STZ 65 mg/kg and the Puerarin-treated rats were intraperitoneally injected Puerarin 100 mg/kg/day for 4 weeks. The results showed the Puerarin could improve body weight, blood sugar, BUN and SCr levels, and reduce ultrastructural changes of kidney in diabetic rats. It also attenuated eNOS expression in glomerular endothelial cells and tubular cells of diabetic rats with Puerarin treatment (p50.05). The Puerarin had significant renal-protective effects for the diabetic nephropathy, possibly through regulating eNOS expression, and it may be used as a potential therapeutic reagent.

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Section: Discussionmentioning

confidence: 99%

The Puerarin improves renal function in STZ-induced diabetic rats by attenuating eNOS expression

Zhang

Wang

et al. 2015

Renal Failure

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“…3-NO-Tyr has been described as a stable marker of oxidative/nitrative stress in inflammatory diseases [234, 235], but its utility as clinical biomarker is still questioned. Some studies showed that 3-NO-Tyr plasma levels are increased in several conditions, such as asthma, diabetes, and cardiovascular diseases, and reduced following therapeutic treatments [236, 237]. Moreover, an involvement of 3-NO-Tyr in age-related neurodegenerative diseases has been suggested [238, 239].…”

Section: Markers Based On Ros-induced Modificationsmentioning

confidence: 99%

Measurement and Clinical Significance of Biomarkers of Oxidative Stress in Humans

Marrocco

Altieri

Peluso

2017

Oxidative Medicine and Cellular Longevity

647

500

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Oxidative stress is the result of the imbalance between reactive oxygen species (ROS) formation and enzymatic and nonenzymatic antioxidants. Biomarkers of oxidative stress are relevant in the evaluation of the disease status and of the health-enhancing effects of antioxidants. We aim to discuss the major methodological bias of methods used for the evaluation of oxidative stress in humans. There is a lack of consensus concerning the validation, standardization, and reproducibility of methods for the measurement of the following: (1) ROS in leukocytes and platelets by flow cytometry, (2) markers based on ROS-induced modifications of lipids, DNA, and proteins, (3) enzymatic players of redox status, and (4) total antioxidant capacity of human body fluids. It has been suggested that the bias of each method could be overcome by using indexes of oxidative stress that include more than one marker. However, the choice of the markers considered in the global index should be dictated by the aim of the study and its design, as well as by the clinical relevance in the selected subjects. In conclusion, the clinical significance of biomarkers of oxidative stress in humans must come from a critical analysis of the markers that should give an overall index of redox status in particular conditions.

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“…RNS react with tyrosine residues at the ortho-position relative to the phenolic hydroxyl group, causing nonenzymatic protein tyrosine nitration. Protein tyrosine nitration is a prominent protein posttranslational modification thought to be important in the pathogenesis of severe asthma, , neurodegenerative disorders, , atherosclerosis, , diabetes, cancer, and normal biological aging. , Tyrosine nitration can alter the protein structure and function, leading to the loss of enzyme or receptor activities. − Moreover, tyrosine nitration may interfere directly or indirectly with phosphorylation of tyrosine residues important for signaling pathways …”

Section: Introductionmentioning

confidence: 99%

Selective Affinity Enrichment of Nitrotyrosine-Containing Peptides for Quantitative Analysis in Complex Samples

et al. 2017

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Protein tyrosine nitration by oxidative and nitrate stress is important in the pathogenesis of many inflammatory or aging-related diseases. Mass spectrometry analysis of protein nitrotyrosine is very challenging because the non-nitrated peptides suppress the signals of the low-abundance nitrotyrosine (NT) peptides. No validated methods for enrichment of NT-peptides are currently available. Here we report an immunoaffinity enrichment of NT-peptides for proteomics analysis. The effectiveness of this approach was evaluated using nitrated protein standards and whole-cell lysates in vitro. A total of 1,881 NT sites were identified from a nitrated whole-cell extract, indicating that this immunoaffinity-MS method is a valid approach for the enrichment of NT-peptides, and provides a significant advance for characterizing the nitrotyrosine proteome. We noted that this method had higher affinity to peptides with N-terminal nitrotyrosine relative to peptides with other nitrotyrosine locations, which raises the need for future study to develop a pan-specific nitrotyrosine antibody for unbiased, proteome-wide analysis of tyrosine nitration. We applied this method to quantify the changes in protein tyrosine nitration in mouse lungs after intranasal poly(I:C) treatment and quantified 237 NT sites. This result indicates that the immunoaffinity-MS method can be used for quantitative analysis of protein nitrotyrosines in complex samples.

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Are nitric oxide-mediated protein modifications of functional significance in diabetic heart? ye'S, -NO’, wh‘Y-NO’t?

Cited by 12 publications

References 118 publications

The Puerarin improves renal function in STZ-induced diabetic rats by attenuating eNOS expression

The Puerarin improves renal function in STZ-induced diabetic rats by attenuating eNOS expression

Measurement and Clinical Significance of Biomarkers of Oxidative Stress in Humans

Selective Affinity Enrichment of Nitrotyrosine-Containing Peptides for Quantitative Analysis in Complex Samples

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