Are primary germ tubes of conidia unique to Erysiphe graminis?

Kunoh, Hitoshi; Itoh, Osamu; Kohno, Mitsuru; Ishizaki, Hiroshi

doi:10.3186/jjphytopath.45.675

Cited by 24 publications

(24 citation statements)

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“…This finding is consistent with the observations that fungal attachment and germination are accompanied by the release of proteins, carbohydrates, lipids, glycoproteins, and peptides from the spores (Tucker and Talbot, 2001), and many of these molecules can trigger general host defense responses (Kiba et al, 1999). In addition, primary germ tubes are also capable of breaching host epidermal walls leading to the initiation of cytoplasmic aggregates in underlying host cells (Kunoh, 1982). Not surprisingly, these general responses to the initial phases of fungal infection have been implicated in non-host resistance as well (Heath, 2000;Jones and Takemoto, 2004).…”

Section: Modulation Of Mla-mediated Response Network Is Dependent Onsupporting

confidence: 90%

“…Stages of Bgh infection in barley are well characterized (Ellingboe, 1972;Kunoh, 1982;Jørgensen, 1988;Clark et al, 1993;Hall et al, 1999), and each stage is a potential recognition point with the possible release of pathogen or plant-derived signaling molecules. Induction of localized responses in underlying barley cells has been shown as early as Bgh conidiospore germination, specifically during primary germ tube formation (Kunoh, 1982;Kruger et al, 2003). Although there is an indication of early fungal recognition by the host, initial conidiospore adhesion, germination, and development of appressoria are not fundamentally different in incompatible and compatible barley-Bgh interactions (Boyd et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

“…The well-defined stages of powdery mildew disease development provide multiple possibilities to interrogate the regulation of host genes in response to Mla-specified incompatible and compatible barley-Bgh interactions (Ellingboe, 1972;Kunoh, 1982;Jørgensen, 1988;Clark et al, 1993). Information on transcript abundance can be used to describe a cellular state and predict functional involvement of genes in the interactions among plants and pathogens (Maleck et al, 2000;Schenk et al, 2000;Mysore et al, 2002;Wan et al, 2002;Puthoff et al, 2003;Tao et al, 2003;van Wees et al, 2003;Whitham et al, 2003;Eulgem et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Interaction-Dependent Gene Expression inMla-Specified Response to Barley Powdery Mildew[W]

2004

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Plant recognition of pathogen-derived molecules influences attack and counterattack strategies that affect the outcome of host-microbe interactions. To ascertain the global framework of host gene expression during biotrophic pathogen invasion, we analyzed in parallel the mRNA abundance of 22,792 host genes throughout 36 (genotype 3 pathogen 3 time) interactions between barley (Hordeum vulgare) and Blumeria graminis f. sp hordei (Bgh), the causal agent of powdery mildew disease. A split-split-plot design was used to investigate near-isogenic barley lines with introgressed Mla6, Mla13, and Mla1 coiledcoil, nucleotide binding site, Leu-rich repeat resistance alleles challenged with Bgh isolates 5874 (AvrMla6 and AvrMla1) and K1 (AvrMla13 and AvrMla1). A linear mixed model analysis was employed to identify genes with significant differential expression (P value < 0.0001) in incompatible and compatible barley-Bgh interactions across six time points after pathogen challenge. Twenty-two host genes, of which five were of unknown function, exhibited highly similar patterns of upregulation among all incompatible and compatible interactions up to 16 h after inoculation (hai), coinciding with germination of Bgh conidiospores and formation of appressoria. By contrast, significant divergent expression was observed from 16 to 32 hai, during membrane-to-membrane contact between fungal haustoria and host epidermal cells, with notable suppression of most transcripts identified as differentially expressed in compatible interactions. These findings provide a link between the recognition of general and specific pathogen-associated molecules in gene-for-gene specified resistance and support the hypothesis that host-specific resistance evolved from the recognition and prevention of the pathogen's suppression of plant basal defense.

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Section: Modulation Of Mla-mediated Response Network Is Dependent Onsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Interaction-Dependent Gene Expression inMla-Specified Response to Barley Powdery Mildew[W]

2004

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“…Conidia, when separated from the conidiophore, germinate to form a short, aseptate germ tube within 0.5-2 h. This first germ tube is referred to as the primary germ tube (PGT) and is implicated in attachment of the conidia to the host surface, dissolution of the leaf cuticle, induction of localized host responses, and penetration of the host epidermal cell wall (Kunoh, 1982). On inductive surfaces, a second, appressorial germ tube (AGT) is formed.…”

mentioning

confidence: 99%

Gene Identification in the Obligate Fungal Pathogen Blumeria graminis by Expressed Sequence Tag Analysis

Thomas

Rasmussen

Glaring

et al. 2001

Fungal Genetics and Biology

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“…The latter participate in the formation of chemical barriers, such as antifungal PR proteins (pathogen-related proteins), phytoalexins, and structural barriers, such as lignified walls [23]. The well-defined stages of powdery mildew disease development provide multiple opportunities to investigate the regulation of host genes in response to Pm-specified incompatible and compatible wheat-Pm interactions [24][25][26][27]. Information on transcript abundance can be used to describe a cellular state and predict the functional involvement of genes in the interactions between plants and pathogens [28][29][30][31][32][33][34][35][36].…”

Section: Functional Annotation Of Differentially Expressed Sequencesmentioning

confidence: 99%

Development of a new wheat microarray from a durum wheat totipotent cDNA library used for a powdery mildew resistance study

Cifarelli¹,

D’onofrio²,

Grillo³

et al. 2013

Cellular and Molecular Biology Letters

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Totipotent cDNA libraries representative of all the potentially expressed sequences in a genome would be of great benefit to gene expression studies. Here, we report on an innovative method for creating such a library for durum wheat (Triticum turgidum L. var. durum) and its application for gene discovery. The use of suitable quantities of 5-azacytidine during the germination phase induced the demethylation of total DNA, and the resulting seedlings potentially express all of the genes present in the genome. A new wheat microarray consisting of 4925 unigenes was developed from the totipotent cDNA library and used to screen for genes that may contribute to differences in the disease resistance of two near-isogenic lines, the durum wheat cultivar Latino and the line 5BIL-42, which are respectively susceptible and resistant to powdery mildew. Fluorescently labeled cDNA was prepared from the RNA of seedlings of the two near-isogenic wheat lines after infection with a single powdery mildew isolate under controlled conditions in the greenhouse. Hybridization to the microarray identified six genes that were differently expressed in the two lines. Four of the sequences could be assigned putative functions based on their similarity to known genes in public databases. Physical mapping of the six genes localized them to two regions of the genome: the centromeric region of chromosome 5B, where the Pm36 resistance gene was previously localized, and chromosome 6B.

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Are primary germ tubes of conidia unique to Erysiphe graminis?

Cited by 24 publications

References 1 publication

Interaction-Dependent Gene Expression inMla-Specified Response to Barley Powdery Mildew[W]

Interaction-Dependent Gene Expression inMla-Specified Response to Barley Powdery Mildew[W]

Gene Identification in the Obligate Fungal Pathogen Blumeria graminis by Expressed Sequence Tag Analysis

Development of a new wheat microarray from a durum wheat totipotent cDNA library used for a powdery mildew resistance study

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