Are quinones producers or scavengers of superoxide ion in cells?

Afanas’ev, Igor B.; Korkina, Liudmila; Suslova, Tatjana B.; Soodaeva, Svetlana

doi:10.1016/0003-9861(90)90439-6

Cited by 27 publications

(11 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As shown in Figure 1A, XO/HPX produced a dose-dependent cell death that was reduced in the hSOD1Tg astrocytes. We then examined whether elevated SOD1 activity also protected astrocytes from oxidative damage induced by menadione, an intracellular superoxide generator (Afanas'ev et al, 1990). The hSOD1Tg astrocytes showed a similar increased resistance to menadione (Fig.…”

Section: Resultsmentioning

confidence: 99%

Astrocytes overexpressing Cu,Zn superoxide dismutase have increased resistance to oxidative injury

Chen

Chan

Swanson

2001

Glia

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Astrocytes overexpressing Cu,Zn superoxide dismutase have increased resistance to oxidative injury

Chen

Chan

Swanson

2001

Glia

View full text Add to dashboard Cite

“…It is interesting to note that noxA mutants were only sensitive to menadione, and not to H 2 O 2 or diamide. The redox-cycling compound menadione exerts its toxic effects by stimulating the intracellular production of superoxide radicals (Afanas'ev et al 1990). The thiol-specific oxidant diamide, on the other hand, specifically oxidizes sulfhydryl groups, such as those in cysteine residues.…”

Section: Discussionmentioning

confidence: 99%

Involvement of the NADH oxidase-encoding noxA gene in oxidative stress responses in Corynebacterium glutamicum

Park

Kim

Lee

2014

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Corynebacterium glutamicum ORF NCgl0328, designated noxA, encodes an NADH oxidase enzyme. The noxA gene, which was preferentially expressed in the log growth phase, was found to be under the control of the whcA, whcB, and whcE genes, which play regulatory roles in cells under oxidative stress. While noxA transcription was minimal in whcE-deleted mutant cells (ΔwhcE) during growth, its transcription was maximal even in the stationary phase in ΔwhcA cells. The transcription levels of noxA in ΔwhcB and whcB-overexpressing cells were comparable to the levels only in the log growth phase in ΔwhcA and whcA-overexpressing cells, respectively. Direct binding of purified WhcA to the promoter region of noxA was observed in vitro. The DNA-protein interaction was only possible in the presence of the reducing agent dithiothreitol. A noxA-deleted mutant strain and a strain overexpressing the noxA gene (P180-noxA) were established, and these strains were found to exhibit defective cell growth. The ΔnoxA and P180-noxA strains were sensitive to the redox-cycling oxidant menadione, suggesting a role of noxA in redox balancing. Accordingly, the purified NoxA enzyme exhibited NADH-oxidizing activity. Taken together, these data show that noxA plays a role in oxidative stress responses and also that the gene is under direct control of the WhcA protein, which was shown to be a regulatory DNA-binding protein. Furthermore, the involvement and roles of the whcA, whcB, and whcE genes in regulating the expression of noxA were demonstrated.

show abstract

“…It is also widely accepted that the action of many quinone compounds in cells is partially or completely due to their capacity to stimulate superoxide production (Afanas'ev et al 1990). …”

Section: Introductionmentioning

confidence: 99%

Respiratory stimulation and generation of superoxide radicals in Pseudomonas aeruginosa by fungal naphthoquinones

Haraguchi

Yokoyama

Oike

et al. 1997

Archives of Microbiology

View full text Add to dashboard Cite

The mechanism of action of antimicrobial naphthoquinones from the fungus Fusarium was studied by using Pseudomonas aeruginosa. Bostricoidin, methyl ether fusarubin, and fusarubin stimulated the oxygen consumption of bacterial cells and induced cyanide-insensitive oxygen consumption. These activities of the tested compounds were also observed in bacterial membrane preparations in a dose-dependent manner. Naphthoquinones stimulated the generation of superoxide anion and hydrogen peroxide. The naphthoquinone effectively acted as the electron acceptors for bacterial diaphorase, which could explain the antibacterial activity of Fusarium naphthoquinones since electron acceptors lead to the stimulation of respiratory activity and the generation of oxygen radical species.

show abstract

Are quinones producers or scavengers of superoxide ion in cells?

Cited by 27 publications

References 16 publications

Astrocytes overexpressing Cu,Zn superoxide dismutase have increased resistance to oxidative injury

Astrocytes overexpressing Cu,Zn superoxide dismutase have increased resistance to oxidative injury

Involvement of the NADH oxidase-encoding noxA gene in oxidative stress responses in Corynebacterium glutamicum

Respiratory stimulation and generation of superoxide radicals in Pseudomonas aeruginosa by fungal naphthoquinones

Contact Info

Product

Resources

About