Are Small RNAs Associated with Crohn’s Disease?

Pechan, Reinhard; Kunert, Hans; Groß, H.

doi:10.1515/znc-1987-7-848

Cited by 5 publications

(2 citation statements)

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“…Intriguingly, abundant ladder‐like small RNAs were found only in CD IEC. The possibility that this is due to a latent viral infection can not be discarded, and an association of CD to small RNA has been previously proposed (54).…”

Section: Discussionmentioning

confidence: 99%

Inflammatory Bowel Disease-Associated Gene Expression in Intestinal Epithelial Cells by Differential cDNA Screening and mRNA Display

Fukushima

Yonezawa

Fiocchi

2003

Inflammatory Bowel Diseases

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Intestinal epithelial cells are actively involved in the pathogenesis of inflammatory bowel disease resulting in an altered functional phenotype. The modulation of epithelial gene expression may occur as a consequence of proliferative, metabolic, immune, inflammatory, or genetic abnormalities. Differential screening of epithelial-cell-derived cDNA libraries (from control, ulcerative colitis, and Crohn's disease epithelial cells) and differential display of mRNA were used for investigation of disease-associated gene expression and modulation. Intestinal epithelial gene expression was successfully analyzed by both approaches. Using differential screening with clones encoding mitochondrial genes, quantitative overexpression was observed in both ulcerative colitis and Crohn's disease, while a unique expression of small RNA was noticed in Crohn's disease cells using Alu-homologous clones. Differential display demonstrated that several genes were differentially displayed among control, ulcerative colitis, and Crohn's disease epithelial cells. This was confirmed by immunohistochemical staining of pleckstrin, desmoglein 2 and voltage-dependent anion channel in control and inflammatory bowel disease mucosal samples. In summary, several inflammatory bowel disease-related associations were found. Since both differential screening and display have advantages and limitations, the combination of both techniques can generate complementary information, facilitate search for novel genes, and potentially identify genes uniquely associated with inflammatory bowel disease.

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Section: Discussionmentioning

confidence: 99%

Inflammatory Bowel Disease-Associated Gene Expression in Intestinal Epithelial Cells by Differential cDNA Screening and mRNA Display

Fukushima

Yonezawa

Fiocchi

2003

Inflammatory Bowel Diseases

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“…The appearance of the illness in clusters of small populations and its slow progress and resistance to antibiotic and antiviral medications have prompted the investigation of nonconventional RNA species as primary pathogenic agents of CD and UC. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) provided evidence for the presence of small circle-like RNAs in tissues from individuals with either CD or UC (Pechan et al 1987). More recently, Lundberg et al (1995) performed similar experiments and did not observe any circular RNA between 75 and 300 nucleotides.…”

Section: Introductionmentioning

confidence: 97%

Characterization of stable RNAs from the resected intestinal tissues of individuals with either Crohn's disease or ulcerative colitis

Roy¹,

Mercure²,

Beuvon³

et al. 1997

Biochim. biol. cell.

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Circular RNAs reminiscent of viroids and the human hepatitis delta virus have been proposed as possible nonconventional pathogens responsible for Crohn's disease and ulcerative colitis, two inflammatory bowel diseases. Consequently, RNA was extracted from various areas of intestinal tissues from individuals with either Crohn's disease or ulcerative colitis as well as several appropriate control diseases, and analyzed by two-dimensional gel electrophoresis. No circular viroid-like RNAs (< 1500 nucleotides) were detected, confirming a previous report that was limited to the investigation of small RNAs (< 300 nucleotides). However, three small, unusually stable, linear RNAs were shown to be associated to both Crohn's disease and ulcerative colitis tissues: a specific 28S ribosomal RNA cleavage product characterized previously; a 5.8S ribosomal RNA conformer; and a fragment homologous to transcripts from DNA CpG islands. The two last RNAs were detected prior to visible morphological tissue alterations, suggesting that they are produced early during the inflammation and that they have value as molecular diagnostic tools for the inflammatory bowel diseases. The potential cellular mechanisms producing these RNAs and their involvement in inflammatory bowel disease are discussed.

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Characterization of RNA with unusual electrophoretic mobility from tissues of patients with Crohn's disease

1995

FEBS Letters

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RNAs from tissues of patients with Crohn's disease that migrate off the diagonal in a two-dimensional gel electrophoresis system were partially characterized. One of the RNA species was a discrete cleavage product ofregion V2-9 of28S rRNA; another is a conformer or variant of S.8S rRNA; and a third is a mixture of unidentified fragments with mobility similar to that of 7S RNA. The yield of these species from resected tissue and their visualization by silver staining was very sensitive to the details of the preparative procedure. No evidence of viroid-like RNA was found within the range of molecular sizes «7S) that we examined.

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Are Small RNAs Associated with Crohn’s Disease?

Cited by 5 publications

References 0 publications

Inflammatory Bowel Disease-Associated Gene Expression in Intestinal Epithelial Cells by Differential cDNA Screening and mRNA Display

Inflammatory Bowel Disease-Associated Gene Expression in Intestinal Epithelial Cells by Differential cDNA Screening and mRNA Display

Characterization of stable RNAs from the resected intestinal tissues of individuals with either Crohn's disease or ulcerative colitis

Characterization of RNA with unusual electrophoretic mobility from tissues of patients with Crohn's disease

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