Are the enzymatic methods currently being used to measure bronchoalveolar lavage bile salt levels fit for purpose?

Parikh, Shruti; Brownlee, Iain A.; Robertson, Andrew; Manning, Nigel; Johnson, Gail; Brodlie, Malcolm; Corris, Paul A.; Ward, Chris; Pearson, Jeffrey P.

doi:10.1016/j.healun.2012.12.008

Cited by 16 publications

(10 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Samples were collected by techniques commonly used clinically and with small variations in the lavage volumes. Bile acid concentrations were measured by mass spectrometry, because other techniques, such as enzymatic methods, are less sensitive for low concentrations …”

Section: Discussionmentioning

confidence: 99%

Reflux aspiration in lungs of dogs with respiratory disease and in healthy West Highland White Terriers

Määttä

Laurila

Holopainen

et al. 2018

Veterinary Internal Medicne

View full text Add to dashboard Cite

BackgroundGastroesophageal reflux and microaspiration (MA) of gastric juice are associated with various human respiratory diseases but not in dogs.ObjectiveTo detect the presence of bile acids in bronchoalveolar lavage fluid (BALF) of dogs with various respiratory diseases.AnimalsTwenty‐seven West Highland White Terriers (WHWTs) with canine idiopathic pulmonary fibrosis (CIPF), 11 dogs with bacterial pneumonia (BP), 13 with chronic bronchitis (CB), 9 with eosinophilic bronchopneumopathy (EBP), 19 with laryngeal dysfunction (LD), 8 Irish Wolfhounds (IWHs) with previous BPs, 13 healthy WHWTs, all privately owned dogs, and 6 healthy research colony BeaglesMethodsProspective cross‐sectional observational study with convenience sampling of dogs. Bile acids were measured by mass spectrometry in BALF samples. Total bile acid (TBA) concentration was calculated as a sum of 17 different bile acids.ResultsConcentrations of TBA were above the limit of quantification in 78% of CIPF, 45% of BP, 62% of CB, 44% of EBP, 68% of LD, and 13% of IWH dogs. In healthy dogs, bile acids were detected less commonly in Beagles (0/6) than in healthy WHWTs (10/13). Concentrations of TBA were significantly higher in CIPF (median 0.013 μM, range not quantifiable [n.q.]‐0.14 μM, P < .001), healthy WHWTs (0.0052 μM, n.q.‐1.2 μM, P = .003), LD (0.010 μM, n.q.‐2.3 μM, P = .015), and CB (0.0078 μM, n.q.‐0.073 μM, P = .018) groups compared to Beagles (0 μM, n.q.).Conclusion and Clinical ImportanceThese results suggest that MA occurs in various respiratory diseases of dogs and also in healthy WHWTs.

show abstract

Section: Discussionmentioning

confidence: 99%

Reflux aspiration in lungs of dogs with respiratory disease and in healthy West Highland White Terriers

Määttä

Laurila

Holopainen

et al. 2018

Veterinary Internal Medicne

View full text Add to dashboard Cite

show abstract

“…Además, tanto pepsina como ácidos biliares de muestras de lavado broncoalveolar (LBA) de pacientes con FPI se detectaron comúnmente y las concentraciones fueron significativamente más altas que las detectadas en pacientes con otras EPI y sujetos de control 17 . Sin embargo, en estos estudios, la bilis se detectó mediante ensayos enzimáticos que no se han validado, específicamente para la medición en líquido del lavado broncoalveolar 20 , por lo tanto, se debe tener precaución al interpretar los resultados.…”

Section: Actualización 2019unclassified

Terapias adyuvantes a la terapia antifibrótica en la fibrosis pulmonar idiopática. Importancia del manejo multidisciplinario

et al. 2019

Rev. chil. enferm. respir.

View full text Add to dashboard Cite

Adjuvant therapies to antifibrotic therapy in IPF. Importance of multidisciplinary management Idiopathic pulmonary fibrosis (IPF) is characterized by reduced functional capacity, dyspnea and exercise-induced hypoxia, which decreases tolerance to exertion and limits the ability to perform daily activities. Comorbidities are frequent and their presence contribute to worsening quality of life and increased mortality. Therefore, in addition to antifibrotic therapies, patients with IPF benefit of a comprehensive approach to care that may include: screening, diagnosis and treatment of comorbidities, admission to research protocols, symptomatic management, palliative care, supplementary oxygen, pulmonary rehabilitation, education and support by a multidisciplinary team.

show abstract

“…Measurement of gastric contents in bronchoalveolar lavage (BAL) is an imperfect science, and the study of GERD in lung dysfunction has been limited by the lack of a reliable and reproducible indicator of GERD in BAL. [1][2][3] In fact, most of the evidence that implicates GERD in native or allograft lung dysfunction is indirect and comes from clinical series that study GERD and preventive effect of antireflux interventions. [4][5][6] Antireflux procedures have been shown to stabilize decline in native lung function in patients with pulmonary fibrosis.…”

mentioning

confidence: 99%

“…To make progress in the study of GERD and its effect on native or allograft lung dysfunction, we have to address the 2 major limitations discussed: (1) We need a reproducible marker of gastric contents that can be measured in BAL; and (2) we need to report GERD and antireflux procedure outcomes in the context of the organ proximal to the gastroesophageal junction and the one distal to it and not be oblivious to their dysfunction.…”

mentioning

confidence: 99%

Commentary: Gastroesophageal reflux and lung allograft dysfunction: Need to improve detection and clinical reporting

Ahmad

Lane

2020

The Journal of Thoracic and Cardiovascular Surgery

View full text Add to dashboard Cite

show abstract

Are the enzymatic methods currently being used to measure bronchoalveolar lavage bile salt levels fit for purpose?

Cited by 16 publications

References 19 publications

Reflux aspiration in lungs of dogs with respiratory disease and in healthy West Highland White Terriers

Reflux aspiration in lungs of dogs with respiratory disease and in healthy West Highland White Terriers

Terapias adyuvantes a la terapia antifibrótica en la fibrosis pulmonar idiopática. Importancia del manejo multidisciplinario

Commentary: Gastroesophageal reflux and lung allograft dysfunction: Need to improve detection and clinical reporting

Contact Info

Product

Resources

About