Are the optimum levels of the catalase and vitamin E in rooster semen extender after freezing-thawing influenced by sperm concentration?

Moghbeli, Morteza; Kohram, Hamid; Zare-Shahaneh, Ahmad; Zhandi, Mahdi; Sharafi, Mohsen; Nabi, Mohammad; Zahedi, Vahid; Sharideh, Hossein

doi:10.1016/j.cryobiol.2016.03.008

Cited by 51 publications

(23 citation statements)

References 33 publications

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“…The obtained results in the current study were in accordance with the preceding findings in diverse mammalian species i.e. domestic pigs, bulls and boars [4,19,22,28]. Thus taken together these elucidation in conjunction with obtained result in the current study, the most probable rational of advanced acrosome integrity rate might be due to the antioxidant characteristics of α-tocopherol that has been associated with maintenance of spermatozoa acrosomal integrity via diminution of the too much ROS formed throughout the cryopreservation procedure [20,21].…”

Section: Discussionsupporting

confidence: 92%

Enhancement of Extender Excellence of Frozen Bull Semen Using α-Tocopherol as an Antioxidant

Ullah

Khan

Hussain

et al. 2019

Acta Scientiae. Vet.

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Background: Addition of the antioxidant to extender media is the most appropriate attempt to reduce structural losses encounter during the process of cryopreservation. Hence semen excellence could be maintained for longer duration without adverse impact. Additionally antioxidants are not only capturing the reactive oxygen species but also improve the sperm quality indicators and fertility. Accordingly, current elucidation has been executed to explore the dose depended appraisal of varied concentration of α-tocopherol in Tris-based extender on frozen-thawed bull semen quality parameters for enhancement of bull semen cryopreservation in the subtropical ecosystem of Peshawar. Materials, Methods & Results:Experiments were carried out on semen that has been collected from both Achai-an indigenous breed and Holstein Friesian (HF) -the exotic breed in artificial vagina maintained at 42°C from the experimental bulls of either breed and processed independently breed wise. Semen specimens with above 70% motility were evaluated separately breed-wise under same environmental condition. Standard procedure was adopted to extend the collected semen in the experimental extenders and frozen subsequently. After thawing, further Analysis of the frozen straws of semen was carried out for sperm excellence indicators that include motility, viability, acrosomal integrity and functional integrity of spermatozoa under the subtropical condition. Sperm viability and acrosomal integrity were determined by dual staining procedure i.e. Trypan-blue and Giemsa stains. The hypo-osmotic swelling (HOS) test was used to assess plasma membrane integrity. The current elucidation demonstrated that α-tocopherol 1.5 mg/mL supplemented in extender had significantly (P < 0.05) increased sperm excellence gauge that includes motility, viability, acrosomal integrity and functional membrane integrity in both the breeds. On the other hand, the result further elucidated those concentrations higher or lower than 1.5 -1 mg/mL α-tocopherol supplemented in the present study resulted in a lower semen functional attributes subsequent to freezing. Discussion: Biological samples were preserved by various methods such cryopreservation at low temperature to maintain them intact and facilitate downstream experiments. Antioxidants were associated with the chemical breakage of the substrate consequential from oxidation and counterbalance the free radicals thus diminish the damaging impacts to spermatozoa in cryopreservation. There is lack of data with respect to lipid peroxidation and cell reinforcement limit in cryopreserved semen, and cryopreservation is related with the generation of receptive oxygen species (ROS) which lead to lipid peroxidation (LPO) of sperm membranes, bringing about lost motility, viability and fertility of sperm. Recently, in the reproductive management of the dairy animals, an important focus of research is to overcome the deleterious impact associated with semen cryopreservation. Elucidation of differential dose depended expression of α-tocopherol...

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Section: Discussionsupporting

confidence: 92%

Enhancement of Extender Excellence of Frozen Bull Semen Using α-Tocopherol as an Antioxidant

Ullah

Khan

Hussain

et al. 2019

Acta Scientiae. Vet.

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“…Protective effects of substances with antioxidant properties on rooster sperm motility and viability were demonstrated Lotfi et al, 2017;. Moreover, the positive association between progressive motility and fertility following insemination was shown in avian (Fattah et al, 2017;Lotfi et al, 2017;Moghbeli et al, 2016). Contradictory results were reported about quercetin effects on sperm characteristics.…”

Section: Discussionmentioning

confidence: 99%

“…A previous study showed that endogenous antioxidant capacity of semen is not sufficient to conquer oxidative damage during storage (Aurich, Schönherr, Hoppe, & Aurich, ). Semen enrichment with various compounds and antioxidants ameliorate detrimental effects of ROS and lipid peroxidation during chilled storage or after freeze–thawing process in different species, including boar (Funahashi & Sano, ), bull (Munsi, Bhuiyan, Majumder, & Alam, ), ram (Maxwell & Stojanov, ), stallion (Aurich et al., ), rooster (Eslami, Ghaniei, & Mirzaei Rad, ; Lotfi, Mehri, Sharafi, & Masoudi, ; Moghbeli et al., ) and cat (Baran et al., ) semen.…”

Section: Introductionmentioning

confidence: 99%

“…Semen enrichment with various compounds and antioxidants ameliorate detrimental effects of ROS and lipid peroxidation during chilled storage or after freeze-thawing process in different species, including boar (Funahashi & Sano, 2005), bull (Munsi, Bhuiyan, Majumder, & Alam, 2007), ram (Maxwell & Stojanov, 1996), stallion (Aurich et al, 1997), rooster Lotfi, Mehri, Sharafi, & Masoudi, 2017;Moghbeli et al, 2016) and cat (Baran et al, 2009) semen.…”

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confidence: 99%

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Quercetin attenuates H₂O₂‐induced toxicity of rooster semen during liquid storage at 4°C

Ghaniei

Eslami

Hashem

et al. 2019

Animal Physiology Nutrition

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Current study was carried out to examine the protective effects of quercetin against toxicity induced by hydrogen peroxide in rooster semen in vitro. Semen samples were collected from ten roosters (Ross 308 broiler breeder males, 32 weeks old) twice a week by abdominal massage method. Samples with ≥70% progressive motility were selected, pooled, diluted and used for the study. Experimental groups consisted of negative control, control that received solvent of quercetin, H2O2 (40 μM) and combination groups which incubated with constant dose of H2O2 (40 μM) plus various levels of quercetin (20, 40 and 80 μM). Measurement of total hydroperoxide (HPO), malondialdehyde (MDA), nitric oxide (NO), total antioxidant capacity (TAC) and superoxide dismutase activity as well as routine sperm tests were done at 0, 24 and 48 hr of storage at 4°C. Results revealed that exposure to hydrogen peroxide significantly increased HPO (138.43 ± 7.32 vs. 66.08 ± 3.97 μmol/g protein), MDA (7.21 ± 0.08 vs. 5.71 ± 2.16 μmol/g protein) and NO (0.367 ± 0.013 vs. 0.215 ± 0.011 μmol/g protein) levels and decreased sperm progressive motility (27.28 ± 1.21 vs. 47.49 ± 1.29%), and amounts of TAC (11.49 ± 0.39 vs. 15.70 ± 0.79 mmol/g protein) compared to control at 24 hr (p < 0.05). Changes at mentioned variables were repeated at 48 hr of storage. Also, co‐administration of quercetin (especially at 40 and 80 μM) with hydrogen peroxide restored the toxic effects of hydrogen peroxide on rooster semen parameters such as primary and secondary lipid peroxidative indicators and other evaluated variables. The study concluded that rooster semen enrichment with quercetin would protect lipid peroxidative and nitrosative hydrogen peroxide‐mediated damage during cold liquid storage of rooster semen.

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“…As a result, treatment with an antioxidant compound can protect against or alleviate the cryopreservation‐induced oxidative stress. A beneficial effect of in vitro antioxidant supplements such as vitamin E, vitamin C and glutathione reductase, superoxide dismutase and catalase on sperm post‐thawed parameters has been demonstrated in several studies (Amini et al., 2015a; Amini et al., 2015b; Moghbeli et al., ). There is a good body of literature on the effect of oral antioxidants on fresh sperm quality (Rengaraj & Hong, ; Ross et al., ; Triques et al., ); however, their effectiveness on frozen‐thawed sperm parameters and fertility is still lacking in literature.…”

Section: Introductionmentioning

confidence: 96%

Orally administered Chrysin improves post‐thawed sperm quality and fertility of rooster

Zhandi

Ansari

Roknabadi

et al. 2017

Reprod Domestic Animals

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Chrysin is a bioflavonoid compound found in passion flower, chamomile, propolis and honey at high levels. Post-thawed sperm quality and fertility of Chrysin-fed roosters were assessed in this study. Twenty 40-week-old male broiler breeders were randomly divided into four groups and fed basal diet supplemented with different levels of Chrysin including 0 (Ch-0), 25 (Ch-25), 50 (Ch-50) or 75 (Ch-75) mg/day for 12 consecutive weeks. Semen samples were weekly collected from 6th to 9th week of experiment to evaluate some sperm quality parameters including total and progressive motility, plasma membrane integrity and functionality (in fresh and post-thawed samples) and mitochondrial activity (only in post-thawed samples). Also, collected semen samples from 10th, 11th and 12th week of experiment were frozen and then artificially inseminated to test fertility rate. According to the results, an improvement in both fresh and post-thawed sperm quality including total [fresh: 88.00 ± 0.58 and 87.25 ± 0.67 (p < .01); post-thawed: 51.07 ± 2.05 and 52.72 ± 1.96 (p < .01)] and progressive motility [fresh: 76.00 ± 0.58 and 78.25 ± 0.65 (p < .01); post-thawed: 40.61 ± 2.01 and 39.88 ± 2.01 (p < .01)], plasma membrane integrity [fresh: 91.60 ± 0.58 and 89.85 ± 0.59 (p < .01); post-thawed: 56.99 ± 1.86 and 54.39 ± 1.86 (p < .01)] and functionality [fresh: 75.40 ± 0.42 and 77.90 ± 0.96 (p < .01); post-thawed: 45.69 ± 1.71 and 46.35 ± 1.71 (p < .01)] was noted for both Ch-50 and Ch-75, respectively, groups compared to control group. Despite no significant change in mitochondrial activity, fertility rate of post-thawed spermatozoa was significantly improved in all Chrysin-fed groups compared to Ch-0 group. In conclusion, oral Chrysin administration to roosters could ameliorate cryopreservation-induced impairment of sperm quality and fertility rate.

show abstract

Are the optimum levels of the catalase and vitamin E in rooster semen extender after freezing-thawing influenced by sperm concentration?

Cited by 51 publications

References 33 publications

Enhancement of Extender Excellence of Frozen Bull Semen Using α-Tocopherol as an Antioxidant

Enhancement of Extender Excellence of Frozen Bull Semen Using α-Tocopherol as an Antioxidant

Quercetin attenuates H₂O₂‐induced toxicity of rooster semen during liquid storage at 4°C

Orally administered Chrysin improves post‐thawed sperm quality and fertility of rooster

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Are the optimum levels of the catalase and vitamin E in rooster semen extender after freezing-thawing influenced by sperm concentration?

Cited by 51 publications

References 33 publications

Enhancement of Extender Excellence of Frozen Bull Semen Using α-Tocopherol as an Antioxidant

Enhancement of Extender Excellence of Frozen Bull Semen Using α-Tocopherol as an Antioxidant

Quercetin attenuates H2O2‐induced toxicity of rooster semen during liquid storage at 4°C

Orally administered Chrysin improves post‐thawed sperm quality and fertility of rooster

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Quercetin attenuates H₂O₂‐induced toxicity of rooster semen during liquid storage at 4°C