Are You at Risk?

Jw,

doi:10.1016/s0031-9406(05)66463-2

Cited by 2 publications

(3 citation statements)

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“…Recently, we have con®rmed that Ku is localized in the nucleus as a heterodimer of Ku70 and Ku80 subunits in interphase cells (Koike et al, 1998). On the other hand, accumulating evidence suggests that Ku is not only localized in the nuclei but also in the cytoplasm (Fewell and Ku , 1996;Grawunder et al, 1996). Thus, we examined the cellular localization of the Ku70 and Ku80 proteins in HeLa-S3 cells using confocal laser scanning microscopy ( Figure 1A).…”

Section: Resultsmentioning

confidence: 99%

“…Indeed, a somatostatin analog induced translocation of Ku80 from the cytosol to the nucleus in colon tumor cells (Tovari et al, 1998). On the other hand, in CV-1 cells the subcellular localization of Ku80 was not a ected by somatostatin-treatment (Fewell and Ku , 1996). The nuclear translocation of Ku80 in the actions of somatostatin might depend on the cell type, although the mechanism of transport of Ku80 between the cytoplasm and nucleus has not yet been clari®ed.…”

Section: Introductionmentioning

confidence: 90%

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Ku80 can translocate to the nucleus independent of the translocation of Ku70 using its own nuclear localization signal

Koike¹,

Ikuta

Miyasaka³

et al. 1999

Oncogene

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Ku antigen is a complex of Ku70 and Ku80 subunits and plays an important role in not only DNA double-strand breaks (DSB) repair and V(D)J recombination, but also in growth regulation. Ku is generally believed to always form and function as heterodimers on the basis of in vitro observations. Here we demonstrate that the localization of Ku80 does not completely coincide with that of Ku70. Ku70 and Ku80 were colocalized in the nucleus in the interphase but not in the late telophase/early G1 phase of the cell cycle. Since the in vivo function of Ku might be partially regulated by the control of its transport, we attempted to investigate the molecular mechanisms underlying the nuclear translocation of Ku. The nuclear translocation of Ku80 started during the late telophase/ early G1 phase after the nuclear envelope was formed and this was preceded by the nuclear translocation of Ku70. Furthermore, we found that the Ku80 protein was transported to the nucleus without heterodimerization with Ku70. To understand in detail the mechanism of transport of Ku80, we attempted to identify the nuclear localization signal (NLS) of Ku80 and de®ned to a region spanning nine amino acid residues (positions 561 ± 569). The Ku80 NLS was demonstrated to be mediated to the nuclear rim by two components of PTAC58 and PTAC97. All these ®ndings support the idea that Ku80 can translocate to the nucleus using its own NLS independent of the translocation of Ku70.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 90%

Ku80 can translocate to the nucleus independent of the translocation of Ku70 using its own nuclear localization signal

Koike¹,

Ikuta

Miyasaka³

et al. 1999

Oncogene

View full text Add to dashboard Cite

show abstract

“…DCs are well suited for this purpose, as they express diverse receptors that mediate the acquisition of antigens in peripheral tissues, process this material efficiently into MHC class I and II pathways, upregulate costimulatory molecules upon maturation, and migrate to secondary lymphoid tissues (Banchereau et al, 2000). These specializations have engendered considerable interest in manipulating DCs to augment antitumor responses (Young and Inaba, 1996).…”

Section: Cytokines In the Tumor Microenvironmentmentioning

confidence: 99%