AreA controls nitrogen source utilisation during both growth programs of the dimorphic fungus Penicillium marneffei

Bugeja, Hayley E; Hynes, Michael J.; Andrianopoulos, Alex

doi:10.1016/j.funbio.2011.10.009

Cited by 21 publications

(18 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The utilization of nonpreferred nitrogen sources has been shown to enhance filamentation at 37°C; however, this enhancement appears to be independent of the global regulator areA, which encodes the GATA transcription factor required for the utilization of nonpreferred nitrogen sources. Deletion of areA does not decrease filamentation or affect yeast cell production (40,48).…”

Section: In Vitro and In Vivo Yeast Morphogenesismentioning

confidence: 99%

Morphogenetic Circuitry Regulating Growth and Development in the Dimorphic Pathogen Penicillium marneffei

Boyce

Andrianopoulos

2013

Eukaryot Cell

Self Cite

View full text Add to dashboard Cite

Penicillium marneffei is an emerging human-pathogenic fungus endemic to Southeast Asia. Like a number of other fungal pathogens, P. marneffei exhibits temperature-dependent dimorphic growth and grows in two distinct cellular morphologies, hyphae at 25°C and yeast cells at 37°C. Hyphae can differentiate to produce the infectious agents, asexual spores (conidia), which are inhaled into the host lung, where they are phagocytosed by pulmonary alveolar macrophages. Within macrophages, conidia germinate into unicellular yeast cells, which divide by fission. This minireview focuses on the current understanding of the genes required for the morphogenetic control of conidial germination, hyphal growth, asexual development, and yeast morphogenesis in P. marneffei. MORPHOGENESIS DURING THE GROWTH AND DEVELOPMENT OF PENICILLIUM MARNEFFEI

show abstract

Section: In Vitro and In Vivo Yeast Morphogenesismentioning

confidence: 99%

Morphogenetic Circuitry Regulating Growth and Development in the Dimorphic Pathogen Penicillium marneffei

Boyce

Andrianopoulos

2013

Eukaryot Cell

Self Cite

View full text Add to dashboard Cite

show abstract

“…The macrophage infection assay was performed as previously described (46). Briefly, J774 murine macrophages were seeded at 1 × 10 5 cells/mL in a flask containing Dulbecco's modified Eagle medium (DMEM) and grown for 2 days.…”

Section: Macrophage Infection Assaymentioning

confidence: 99%

“…Full details of strains used in this study are listed in Supplementary Table 3. The ΔpepA (G1004), ΔpopH (G994) and ΔpopO (G997) strains were generated by transforming strain G816 (ΔligD, pyrG1) [46] with linearised deletion constructs pHW7886 (pepA), pHW7881 (popH), pHW7882 (popO) and selecting for uracil prototrophy. The ΔpopS (G1002) strain was generated by transforming strain G809 (ΔligD::pyrG, pyrG1) with linearised deletion construct pHW7884 (popS) and selecting for glufosinate resistance.…”

mentioning

confidence: 99%

A unique aspartyl protease gene expansion in Talaromyces marneffei plays a role in growth inside host phagocytes

et al. 2019

Self Cite

View full text Add to dashboard Cite

Aspartyl proteases are a widely represented class of proteolytic enzymes found in eukaryotes and retroviruses. They have been associated with pathogenicity in a range of disease-causing microorganisms. The dimorphic human-pathogenic fungus Talaromyces marneffei has a large expansion of these proteases identified through genomic analyses. Here we characterize the expansion of these genes (popparalogue of pep) and their role in T. marneffei using computational and molecular approaches. Many of the genes in this monophyletic family show copy number variation and positive selection despite the preservation of functional regions and possible redundancy. We show that the expression profile of these genes differs and some are expressed during intracellular growth in the host. Several of these proteins have distinctive localization as well as both additive and epistatic effects on the formation of yeast cells during macrophage infections. The data suggest that this is a recently evolved aspartyl protease gene family which affects intracellular growth and contributes to the pathogenicity of T. marneffei. ARTICLE HISTORY

show abstract

“…Thus, a single crossover event between the start codon and the mutated region of the genomic allele leads to integration of the plasmid and restoration of gene function, that is the ability to grow in the absence of uracil or on nitrate as a sole nitrogen source (Table 2). Additionally, the areA gene, encoding the GATA transcription factor required for growth on non-preferred nitrogen sources, was also developed as a locus for targeted integration (Bugeja et al, 2012b). This locus displays a high-rate of homologous integration and has been modified such that the DNA-binding domain has been deleted (areA ∆DBD ), resulting in loss of gene function, yet the majority of the coding region is still intact.…”

Section: Targeted Integration Of Plasmidsmentioning

confidence: 99%

Strategies for the molecular genetic manipulation and visualization of the human fungal pathogen Penicillium marneffei

Boyce¹,

Bugeja²,

Weerasinghe³

et al. 2012

Fungal Genetics Reports

Self Cite

View full text Add to dashboard Cite

P. marneffei has been established as an experimentally amenable system to study morphogenesis and pathogenicity. This paper describes the development of a number of tools, including numerous selectable markers, to expand the ease with which it can be genetically manipulated. Combined with strains engineered for homologous recombination of exogenous DNA, these tools facilitate efficient molecular genetic studies.

show abstract

AreA controls nitrogen source utilisation during both growth programs of the dimorphic fungus Penicillium marneffei

Cited by 21 publications

References 52 publications

Morphogenetic Circuitry Regulating Growth and Development in the Dimorphic Pathogen Penicillium marneffei

Morphogenetic Circuitry Regulating Growth and Development in the Dimorphic Pathogen Penicillium marneffei

A unique aspartyl protease gene expansion in Talaromyces marneffei plays a role in growth inside host phagocytes

Strategies for the molecular genetic manipulation and visualization of the human fungal pathogen Penicillium marneffei

Contact Info

Product

Resources

About