1998
DOI: 10.1046/j.1471-4159.1998.70031189.x
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Arecoline Desensitizes Carbachol‐Stimulated Phosphatidylinositol Breakdown in Rat Brain Cortices

Abstract: To understand the effects of arecoline administration on the muscarinic cholinergic signaling pathway, rats were injected with arecoline, 10 mg/kg i.p., and the carbachol‐stimulated phosphoinositide breakdown in rat brain cortical slices was examined. In vivo administration of arecoline resulted in inhibition of carbachol‐stimulated phosphoinositide turnover in rat brain cortical slices. Arecoline was a partial agonist with peak effects of 30% of the maximum as obtained with carbachol. Coaddition of arecoline … Show more

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Cited by 4 publications
(5 citation statements)
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“…Effect of arecoline on CA secretion evoked by ACh, excess K + , DMPP and McN-A-343 from the perfused rat adrenal glands After perfusion with oxygenated Krebs bicarbonate solution for 1 h, basal CA release from the isolated perfused rat adrenal glands amounted to 23.1±2.2 ng per 2 min (n=6). Because the addition of arecoline in rat brain cortical slices inhibited the carbach ol-stimulated phosphoinositide breakdown [15] , we initially attempted to examine the effects of arecoline itself on CA secretion from the perfused model of the rat adrenal glands. However, in the present study, arecoline (1×10 -4 -1×10 -3 mol/L) by itself did not produce any effect on basal CA output of the perfused rat adrenal glands (data not shown).…”
Section: Resultsmentioning
confidence: 99%
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“…Effect of arecoline on CA secretion evoked by ACh, excess K + , DMPP and McN-A-343 from the perfused rat adrenal glands After perfusion with oxygenated Krebs bicarbonate solution for 1 h, basal CA release from the isolated perfused rat adrenal glands amounted to 23.1±2.2 ng per 2 min (n=6). Because the addition of arecoline in rat brain cortical slices inhibited the carbach ol-stimulated phosphoinositide breakdown [15] , we initially attempted to examine the effects of arecoline itself on CA secretion from the perfused model of the rat adrenal glands. However, in the present study, arecoline (1×10 -4 -1×10 -3 mol/L) by itself did not produce any effect on basal CA output of the perfused rat adrenal glands (data not shown).…”
Section: Resultsmentioning
confidence: 99%
“…However, arecoline appears to be a full agonist in membranes prepared from rat cortical slices regardless of guanosine 5'-O-(3-thiotriphosphate) concentrations [14] . To assess the influence of arecoline treatment on muscarinic receptor-associated phosphoinositide signaling pathways, Lee and colleagues [15] investigated the effects of acute and chronic administration of arecoline on muscarinic cholinergic receptor-stimulated phosphoinositide turnover in rat brain cortical slices. They found that administration of arecoline inhibited carbachol-stimulated phosphoinositide turnover.…”
Section: Discussionmentioning
confidence: 99%
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“…Although both arecoline and carbachol are agonists for muscarinic receptors, in our study, we found that the inhibitory effects of the arecoline and the carbachol on the ACC neurons are slightly different. Previous studies reported that the arecoline is a partial agonist with peak effects of 30% of the maximum as obtained with carbachol [ 42 ]. In the rat cortices, administration of arecoline inhibited carbachol-stimulated phosphoinositide turnover.…”
Section: Discussionmentioning
confidence: 99%
“…This could open voltage-dependent Ca 2+ channels and induce the release of a neurotransmitter that acts at a receptor directly coupled to the phosphoinositide response. Arecoline-stimulated desensitization is associated with sequestration of cell surface receptors without proteolytic degradation of internalized receptors [ 42 ]. Therefore, arecoline appears as a partial agonist in whole cells.…”
Section: Discussionmentioning
confidence: 99%