Arg-Phe-amide-like peptides in the primitive nervous systems of coelenterates

Grimmelikhuijzen, Cornelis J.P.; Graff, D.

doi:10.1016/0196-9781(85)90417-6

Cited by 52 publications

(26 citation statements)

References 28 publications

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“…Since that report, many bioactive peptides with the signature "-RFamide" in the C-terminus have been described from nearly all groups of invertebrates and vertebrates (reviewed by Greenberg and Price, 1992;Mercier et al ., 2003), including coelenterates (Grimmelikhuijzen and Graff, 1985), worms (Maule et al ., 1996), molluscs (Santama et al ., 1995), insects (White et al ., 1986;Schneider et al ., 1993a;Lange et al ., 1994;Nichols et al ., 1999;Taghert, 1999), and other arthropods (Huybrechts et al ., 2003) and vertebrates (Dockray et al ., 1983;Perry et al ., 1997). In fact, experiments using antiserum developed against the molluscan FMRFamide have shown that nearly all animals have different cells that express FMRFamiderelated peptides (Schneider and Taghert, 1990).…”

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confidence: 99%

Molecular Characterization of a cDNA from the Silk Moth Bombyx mori Encoding Manduca sexta Allatotropin Peptide

et al. 2002

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Section: Introductionmentioning

confidence: 99%

Molecular Characterization of a cDNA from the Silk Moth Bombyx mori Encoding Manduca sexta Allatotropin Peptide

et al. 2002

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“…FMRFamide was first discovered as a cardioexcitatory agent in the Venus clam Macrocallista nimbosa (Price and Greenberg, 1977), and although the distribution of FMRFamide itself appears to be limited to molluscs (Price, 1986), immunoreactivity to FMRFamide has been reported in all the major animal phyla and a number of FMRFamide-like peptides have been isolated from coelenterates (Grimmelikhuijzen and Graff, 1985), arthropods (Boer et al, 1980), and chordates (Dockray et al, 1983;Yang et al, 1985), indicating that FMRFamide is one of a family of related neuropeptides. However, the antibody used in many of these surveys does cross-react with other Arg-Phe-NH, peptides.…”

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confidence: 99%

Cardioactive neuropeptide Phe-Met-Arg-Phe-NH2 (FMRFamide) and novel related peptides are encoded in multiple copies by a single gene in the snail Lymnaea stagnalis

Linacre¹,

Kellett²,

Saunders³

et al. 1990

J. Neurosci.

112

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The neuropeptide Phe-Met-Arg-Phe-NH2 (FMRFamide) is a potent cardioactive neuropeptide in Lymnaea stagnalis. Isolation and sequencing of 2 cDNAs and a genomic clone shows that a single gene encodes a precursor protein which contains 9 copies of the FMRFamide peptide, 2 copies of the related peptide Phe-Leu-Arg-Phe-NH2 (FLRFamide), and single copies of the putative pentapeptides Gln-Phe- Tyr-Arg-lle-NH2 (posttranslationally modified to pQFYRlamide) and Glu- Phe-Leu-Arg-lle-NH2 (EFLRlamide). The gene is transcribed in the CNS and gives rise to a single RNA of 1.7 kb in size. The organization of the Lymnaea gene is significant with respect to the evolution of FMRFamide and related peptides in other organisms.

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“…Using this radioimmunoassay and applying cation-exchange chromatography and HPLC, we (2,3,8,9). This paper describes the development of a radioimmunoassay for the coelenterate RFamide-like peptides and the purification and sequencing of such a peptide from the sea anemone Anthopleura elegantissima.…”

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confidence: 99%

“…Ultrastructural and electrophysiological studies have shown that most synapses in the nervous systems of coelenterates are chemical (for reviews see refs. [1][2][3]. Despite this evidence, however, the nature of neurotransmitter substances in coelenterates has remained unknown.…”

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Isolation of pyroGlu-Gly-Arg-Phe-NH2 (Antho-RFamide), a neuropeptide from sea anemones.

Grimmelikhuijzen¹,

Graff²

1986

Proc. Natl. Acad. Sci. U.S.A.

100

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A radioimmunoassay has been developed for peptides containing the carboxyl-terminal sequence Arg-Phe-NH2 (RFamide). Using this radioimmunoassay and applying cation-exchange chromatography and HPLC, we (2,3,8,9). This paper describes the development of a radioimmunoassay for the coelenterate RFamide-like peptides and the purification and sequencing of such a peptide from the sea anemone Anthopleura elegantissima. METHODSRadioimmunoassay. Four rabbits were immunized with RFamide (Bachem, Bubendorf, Switzerland) that was coupled by carbodiimide to bovine thyroglobulin (Sigma). The antisera were treated overnight at 4°C with bovine thyroglobulin at 1 mg/ml, followed by centrifugation, to remove antibody populations specific for the carrier protein. One antiserum (code 145IV) was selected for the present study.125I-labeled Tyr-Phe-Met-Arg-Phe-NH2 was used as a tracer in the radioimmunoassay. It was prepared by adding 5 1.d (0.5 mCi; 18 MBq) of Na125I (Amersham) to 15 ,ul of 0.2 M sodium phosphate buffer, pH 7.0, containing 0.22 ,ug of Tyr-PheMet-Arg-Phe-NH2 (Peninsula Laboratories, San Carlos, CA), followed by addition of 10 ul of phosphate buffer containing 1.4 ,g of chloramine-T. After mixing, the reaction was stopped after 20 sec with 500 1.l of 0.3% Na2S205. The mixture was immediately loaded on a methanol/watertreated Sep-Pak C18 cartridge (Waters Associates), which was quickly rinsed with 40 ml of twice-distilled water, to remove unreacted 1251. Labeled material was eluted with 2-ml portions of 40%, 60%, 80%, and 90% (vol/vol) methanol and three times with 2-ml portions of 100% methanol. Each fraction was collected in 6 ml ofRIA buffer, consisting of0.05 M sodium phosphate at pH 7.4 and 1% bovine serum albumin (Sigma). The major portion of the tracer was eluted at 90% or 100% methanol. The quality of an aliquot (3000 cpm) of the tracer fractions was tested by overnight incubation, at 40C, in

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Arg-Phe-amide-like peptides in the primitive nervous systems of coelenterates

Cited by 52 publications

References 28 publications

Molecular Characterization of a cDNA from the Silk Moth Bombyx mori Encoding Manduca sexta Allatotropin Peptide

Molecular Characterization of a cDNA from the Silk Moth Bombyx mori Encoding Manduca sexta Allatotropin Peptide

Cardioactive neuropeptide Phe-Met-Arg-Phe-NH2 (FMRFamide) and novel related peptides are encoded in multiple copies by a single gene in the snail Lymnaea stagnalis

Isolation of pyroGlu-Gly-Arg-Phe-NH2 (Antho-RFamide), a neuropeptide from sea anemones.

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