Argentatin B derivatives induce cell cycle arrest and DNA damage in human colon cancer cells through p73/p53 regulation

Cycloartane- and Lanostane-Type Triterpenoids from the Resin of Parthenium argentatum AZ-2, a Byproduct of Guayule Rubber Production

“…Initially, a test concentration of 35 μM was selected based on recently reported in vitro cytotoxic activity for argentatin A ( 14 ). 13 Cytotoxicity assay data revealed that at this concentration most of the triterpenoids showed weak to moderate inhibition (Table S1 in the Supporting Information ). Among these, argentatin H ( 13 ), argentatin A ( 14 ), and argentatin B ( 18 ) were selected for further evaluation based on their activities and availability.…”

“…It was also found that the cytotoxic activity (IC 50 = 31.9–35.0 μM) of argentatin A ( 14 ) for the cell lines tested for 72 h is slightly better than the activity (IC 50 = 43.2–61.3 μM) reported for 14 for a series of colon cancer cell lines. 13 It is also noteworthy that the cytotoxic activity of argentatin B ( 18 ) is ca. 2-fold higher than that of argentatin A ( 14 ) for some cancer cell lines, suggesting the possible effect of structure on cytotoxic activity of these triterpenoids.…”

“…Previous reports of cytotoxic activity of cycloartane and lanostane types of triterpenoids including argentatins prompted us to evaluate 1 – 22 for their cytotoxic activity in a panel of three sentinel human cancer cell lines MCF-7 (breast adenocarcinoma), NCI-H460 (non-small cell lung), and SF-268 (CNS glioma), two metastatic cancer cell lines MDA-MB-231 (breast adenocarcinoma) and PC-3M (prostate adenocarcinoma), and normal lung epithelial cells (WI-38). Initially, a test concentration of 35 μM was selected based on recently reported in vitro cytotoxic activity for argentatin A ( 14 ) . Cytotoxicity assay data revealed that at this concentration most of the triterpenoids showed weak to moderate inhibition (Table S1 in the Supporting Information).…”

“…The ability of guayule resin to protect wood from termite attack has been reported, 11 and subsequent studies have demonstrated moderate termite antifeedant activity of argentatin B. 12 Several argentatins have been reported to exhibit weak cytotoxic 13 and antimicrobial activities. 14 Importantly, despite its weak in vitro cytotoxic activity, argentatin A has recently been demonstrated to have promising in vivo antitumor activity in a mouse xenograft model of human colon cancer at high doses with no adverse toxicity.…”

Cytotoxic Property of Grias neuberthii Extract on Human Colon Cancer Cells: A Crucial Role of Autophagy

“…Cell Cycle Analysis. Cell cycle distribution was evaluated using propidium iodide (PI, P4170, Sigma Aldrich, USA) staining processed according to the previous protocol, Bailon-Moscoso et al [11]. In summary, cells were seeded in 6-well plates at a density of 1 × 10 6 cells in 2 mL of medium per well and incubated for 24 h. Cells were then treated for 48 h with 20, 30, and 50 μg/mL of GNSbM extract or 0.3 μg/ mL Dxo.…”