Arginase activity and nitric oxide levels may be considered as tumor markers in breast cancer

Abstract

To investigate the relationship of breast cancer with serum arginase activity and nitric oxide levels. Arginase (L-arginine ureohidrolase, EC. 3.5.3.1) is the last enzyme of urea cycle which converts arginine into urea and ornithine. nitric oxide, a product of L-arginine and nitric oxide synthetase reaction, is a hormone, a reactive oxygen species, neurotransmitter, mediator, cytoprotective molecule, and the only endogenous molecule that acts as a cytotoxic molecule. This study was done at Gaziantep University Research Hospital with 30 breast cancer patients (30-77) and with 34 healthy people (30-75) to diagnose breast cancer. The majority (n=28) was ductal while the rest were medullary and papillary cancers. Serum arginase activity was measured by thiosemicarbazide diasetilmonoksim urea method in U/L that was modified. Also, serum nitric oxide levels were measured by the Griess method in terms of mmol/L. Serum arginase activity was determined as 17.8±2.5 (X±SE) U/L in the patient group and 6.8±0.9 U/L in the control group; the cancer patients showed a significant increase (t=3,649, p <0.01). Serum nitric oxide levels were found 139.4 ± 7.4 mmol / L in the breast cancer group, and 95.9 ± 7.6.1 mmol/L in the healthy group. nitric oxide levels were found to be significant in the analysis (t=4,197, p<0.001). In this study, a significant increase in serum arginase and nitric oxide activity was observed for the breast cancer patients, and it was concluded that both could be important for the diagnosis of breast cancer and for its treatment.

Biochemical role of phytoestrogen (Chrysin) against N-methyl nitrosourea (NMU)- induced proliferative lesions in mammary glands of postmenopausal female rat

“…The present results are in well accordance with the previous findings of polat et al (2003) and Geyikli et al (2012) according to which the arginase activity was elevated in the serum of women with BC in comparison to healthy women. It seems also in the present study that consumption of chrysin as treatment (groups 4 & 5) brought back the arginase activity to normal levels in comparison to the protective (groups 6 & 7).…”

Oxidative stress and some biochemical parameters during starvation and refeeding in Astacus leptodactylus (Esch., 1823)

“…The AR competes with inducible NOS, the highoutput, inducible pathway for increased production of •NO, for L -Arg, their common substrate in multiple cell types including endothelial cells. In cases in which L -Arg is limited, •NO levels can reduction, and this may be the result of increased AR activity (5,43). In the some crustaceans has been determined AR activities in the midgut, gill, muscle and hemolymph besides hepatopancreas (44,45).…”

“…Nitric oxide is produced from arginine by nitric oxide synthase (NOS). Arginine is the sole substrate of NOS (4,5).…”