Arginase inhibitory and antioxidant activities in Syzygium cumini (L.) Skeels leaves extracts collected from three different locations of java

Abstract

Arginase inhibition and antioxidant activities may improve endothelial dysfunction. Previous studies demonstrated that Syzygium cumini leaves had pharmacological effects in diseases related to endothelial dysfunction, but there was no source linking the effect with arginase inhibitory activity. The aim of this study was to investigate arginase inhibitory and antioxidant activity of S. cumini leaves extract from different sources of simplisia, also determine its total phenolic content and gallic acid concentration. The leaves of S. cumini collected from Tangerang, Bogor and Sukoharjo city in Java, Indonesia. Arginase inhibition assay was done in vitro using colorimetric determination of urea which generated from L-arginine and arginase reaction. Antioxidant activity was evaluated using DPPH and FRAP methods. Total phenolic content of extract determined using Folin-Ciocalteu method. Gallic acid concentration was estimates using HPTLC. At a concentration of 50 µg/mL, all extracts exhibited arginase inhibition above 80%. However, the most active extract was sample collected from Bogor > Tangerang > Sukoharjo. Antioxidant activity of extract also came in that order. Determination of total phenolic content and gallic acid concentration of extracts revealed that S. cumini leaves collected from Bogor had the highest phenolic content (476.18 mgGAE/g) and gallic acid concentration (4.4 mg/g of extract). S. cumini leaves from different sources possessed different activity value on arginase inhibition, but not significant statistically. The leaves extract from Bogor, with the highest phenolic content and gallic acid concentration, showed the highest arginase inhibitory and antioxidant activity. Research Article

Perbedaan Aktivitas Antioksidan Ekstrak Daun Jamblang Berdasarkan Perbedaan Ketinggian Tempat Tumbuh Menggunakan Metode Spektrometri Massa : Tinjauan Literatur

“…Berdasarkan hasil literature review yang di dapatkan yaitu Hidayati et al, (2020) dengan judul "Arginase inhibitory and antioxidant activities in Syzygium cumini (L.) Skeels leaves extracts collected from three different locations of java". Disfungsi endotel dapat diperbaiki dengan aktivitas antioksidan dan penghambatan arginase.…”

"Review: Studi Kandungan Fitokimia, Aktivitas Antioksidan, dan Toksisitas Jamblang (Syzygium cumini L.)"

“…Sampel diambil dari Tangerang, Bogor, dan Sukoharjo dimana secara berurutan menghasilkan nilai IC 50 sebesar 17,67 μg/ml, 14,84 μg/ml, dan 19,98 μg/ml dengan kategori kekuatan antioksidan sangat kuat (IC 50 < 50). Sampel Bogor memiliki kandungan fenolik tertinggi (476,18 mgGAE/g) dan konsentrasi asam galat (4,4 mg/g ekstrak) yang bertanggung jawab sebagai aktivitas antioksidan [18].…”