Arginine-conjugated polypropylenimine dendrimer as a non-toxic and efficient gene delivery carrier

Kim, Tae Il; Baek, Jung-Un; Bai, Cheng Zhe; Park, Jong‐Sang

doi:10.1016/j.biomaterials.2006.12.013

Cited by 146 publications

(86 citation statements)

References 26 publications

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“…Dendrimers have been shown to be nontoxic, and they are highly efficient carriers for the delivery of nucleic acids and short oligodeoxynucleotides. [17][18][19] Ferenc et al proved that phosphorus dendrimers have the potential to become efficient carriers of small interfering RNA in anti-HIV therapy. 20 Such complexes have been called dendriplexes by analogy with polyplexes (polymer/nucleic acid complexes) and lipoplexes (liposome/nucleic acid complexes).…”

Section: Discussionmentioning

confidence: 99%

Efficacy and safety of dendrimer nanoparticles with coexpression of tumor necrosis factor-α and herpes simplex virus thymidine kinase in gene radiotherapy of the human uveal melanoma OCM-1 cell line

Wang¹,

Li²,

Wei³

et al. 2013

IJN

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Section: Discussionmentioning

confidence: 99%

Efficacy and safety of dendrimer nanoparticles with coexpression of tumor necrosis factor-α and herpes simplex virus thymidine kinase in gene radiotherapy of the human uveal melanoma OCM-1 cell line

Wang¹,

Li²,

Wei³

et al. 2013

IJN

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“…We used three methods [25][26][27] to prepare HM-loaded micelles when the amount was fixed to 10 mg Lac-TPCS and 1 mg HM.…”

Section: Screening Of Different Methodsmentioning

confidence: 99%

Application of the central composite design to optimize the preparation of novel micelles of harmine

Bei¹,

Zhou²,

You³

et al. 2013

IJN

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Lactose-palmitoyl-trimethyl-chitosan (Lac-TPCS), a novel amphipathic self-assembled polymer, was synthesized for administration of insoluble drugs to reduce their adverse effects. The central composite design was used to study the preparation technique of harmine (HM)-loaded self-assembled micelles based on Lac-TPCS (Lac-TPCS/HM). Three preparation methods and single factors were screened, including solvent type, HM amount, hydration volume, and temperature. The optimal preparation technique was identified after investigating the influence of two independent factors, namely, HM amount and hydration volume, on four indexes, ie, encapsulation efficiency (EE), drug-loading amount (LD), particle size, and polydispersity index (PDI). Analysis of variance showed a high coefficient of determination of 0.916 to 0.994, thus ensuring a satisfactory adjustment of the predicted prescription. The maximum predicted values of the optimal prescription were 91.62%, 14.20%, 183.3 nm, and 0.214 for EE, LD, size, and PDI, respectively, when HM amount was 1.8 mg and hydration volume was 9.6 mL. HM-loaded micelles were successfully characterized by Fourier-transform infrared spectroscopy, differential scanning calorimetry, X-ray diffraction, and a fluorescencequenching experiment. Sustained release of Lac-TPCS/HM reached 65.3% in 72 hours at pH 7.4, while free HM released about 99.7% under the same conditions.

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“…The major approach in non-viral gene therapy is based on cationic polymers, such as polyethylenimine (PEI) (Yamada et al, 2014), polylysine (PLL) (Thiersch et al, 2013), polypropylenimine (PPI) (Kim et al, 2007) and polyamidoamine (PAMAM) (Zhang et al, 2010). Recent studies have demonstrated that polyamidoamine (PAMAM) can bind negative charged protein due to the electrostatic interaction of polymers and protein (Shcharbin et al, 2007;Froehlich et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

EGF-coated nano-dendriplexes for tumor-targeted nucleic acid deliveryin vivo

Chen

Liu

et al. 2015

Drug Delivery

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The clinical success of therapeutic DNA is still hindered due to the lack of effective delivery carriers. Here, we designed a tumor-targeted gene nano delivery system based on EGFR targeting strategy. Epidermal growth factor (EGF) was introduced to nano-complexes of PAMAM dendrimer and DNA via electrostatic interactions to form self-assembled PAMAM/DNA/ EGF nano-complexes. The properties of self-assembled complexes were characterized by gel retardation assay and particle size and zeta potential analysis. Meanwhile, the toxicity of EGFdendriplexes was evaluated by the MTT assay, which indicated that the complexes exhibited decreased cytotoxicity with the incorporation of EGF. We labeled polyamidoamine (PAMAM) dendrimers with FITC or a near-infrared (NIR) dye Lss670 and tested the cellular uptake in vitro and biodistribution in xenograft mouse tumor models. As compared to dendriplexes, the ternary EGF-dendriplexes showed a significantly higher cellular uptake into HepG2 cells due to the specific binding between EGF and EGF receptor (EGFR) over expressed on HepG2 cells, which resulted in the enhanced gene transfection efficiency. The biodistribution of EGF-dendriplexes in vivo was monitored with in vivo imaging technique, which indicated that EGF-dendriplexes enhanced EGFR-positive tumor-targeted biodistribution. These findings indicate that this novel nano-vector realized efficiently tumor-targeting gene delivery and high efficient gene expression in vivo, and it may possess a potential targeting gene delivery system in cancer therapy.

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Arginine-conjugated polypropylenimine dendrimer as a non-toxic and efficient gene delivery carrier

Cited by 146 publications

References 26 publications

Efficacy and safety of dendrimer nanoparticles with coexpression of tumor necrosis factor-α and herpes simplex virus thymidine kinase in gene radiotherapy of the human uveal melanoma OCM-1 cell line

Efficacy and safety of dendrimer nanoparticles with coexpression of tumor necrosis factor-α and herpes simplex virus thymidine kinase in gene radiotherapy of the human uveal melanoma OCM-1 cell line

Application of the central composite design to optimize the preparation of novel micelles of harmine

EGF-coated nano-dendriplexes for tumor-targeted nucleic acid deliveryin vivo

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Arginine-conjugated polypropylenimine dendrimer as a non-toxic and efficient gene delivery carrier

Cited by 146 publications

References 26 publications

Efficacy and safety of dendrimer nanoparticles with coexpression of tumor necrosis factor-&alpha; and herpes simplex virus thymidine kinase in gene radiotherapy of the human uveal melanoma OCM-1 cell line

Efficacy and safety of dendrimer nanoparticles with coexpression of tumor necrosis factor-&alpha; and herpes simplex virus thymidine kinase in gene radiotherapy of the human uveal melanoma OCM-1 cell line

Application of the central composite design to optimize the preparation of novel micelles of harmine

EGF-coated nano-dendriplexes for tumor-targeted nucleic acid deliveryin vivo

Contact Info

Product

Resources

About

Efficacy and safety of dendrimer nanoparticles with coexpression of tumor necrosis factor-α and herpes simplex virus thymidine kinase in gene radiotherapy of the human uveal melanoma OCM-1 cell line

Efficacy and safety of dendrimer nanoparticles with coexpression of tumor necrosis factor-α and herpes simplex virus thymidine kinase in gene radiotherapy of the human uveal melanoma OCM-1 cell line