Arginine exposure alters ectonucleotidase activities and morphology of zebrafish larvae (<i>Danio rerio</i>)

Capiotti, Katiúcia Marques; Fazenda, Lidiane; Nazario, Luiza Reali; Menezes, Fabiano Peres; Kist, Luiza Wilges; Bogo, Maurı́cio Reis; Silva, Rosane Souza da; Wyse, Ângela T. S.; Bonan, Carla Denise

doi:10.1016/j.ijdevneu.2012.09.002

Cited by 19 publications

(13 citation statements)

References 45 publications

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“…For instance, doxorubicin shows cardiotoxicity in zebrafish at concentrations about 25 mg/l [61] and about 25 mg/kg in mice [62], which proves the method of testing chemicals in the embryonic medium as valid and valuable. Notably, exposure to various amino acids, including arginine, has been tested in zebrafish and validated the model [63].…”

Section: Norvaline Toxicity In Zebrafishmentioning

confidence: 99%

Acute hypoxia elevates arginase 2 and induces polyamine stress response in zebrafish via evolutionarily conserved mechanism

Banerjee

Khrystoforova

Polis

et al. 2021

Cell. Mol. Life Sci.

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Living organisms repeatedly encounter stressful events and apply various strategies to survive. Polyamines are omnipresent bioactive molecules with multiple functions. Their transient synthesis, inducible by numerous stressful stimuli, is termed the polyamine stress response. Animals developed evolutionarily conserved strategies to cope with stresses. The urea cycle is an ancient attribute that deals with ammonia excess in terrestrial species. Remarkably, most fish retain the urea cycle genes fully expressed during the early stages of development and silenced in adult animals. Environmental challenges instigate urea synthesis in fish despite substantial energetic costs, which poses the question of the urea cycle's evolutionary significance. Arginase plays a critical role in oxidative stress-dependent reactions being the final urea cycle enzyme. Its unique subcellular localization, high inducibility, and several regulation levels provide a supreme ability to control the polyamine synthesis rate. Notably, oxidative stress instigates the arginase-1 activity in mammals. Arginase is also dysregulated in aging organisms' brain and muscle tissues, indicating its role in the pathogenesis of age-associated diseases. We designed a study to investigate the levels of the urea cycle and polyamine synthesis-related enzymes in a fish model of acute hypoxia. We evidence synchronized elevation of arginase-2 and ornithine decarboxylase following oxidative stress in adult fish and aging animals signifying the specific function of arginase-2 in fish. Moreover, we demonstrate oxidative stress-associated polyamine synthesis' induction and urea cycle' arrest in adult fish. The subcellular arginase localization found in the fish seems to correspond to its possible evolutionary roles.

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Section: Norvaline Toxicity In Zebrafishmentioning

confidence: 99%

Acute hypoxia elevates arginase 2 and induces polyamine stress response in zebrafish via evolutionarily conserved mechanism

Banerjee

Khrystoforova

Polis

et al. 2021

Cell. Mol. Life Sci.

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“…Diazepam and midazolam did not promote a significant difference on NTPDase activities (Table 2). Similarly, diazepam also did not alter the ecto-5 0 -nucleotidase activity, but midazolam was able to modulate the ecto-5 0 -nucleotidase activity, promoting a reduction in AMP Leite et al (2013), c design by authors, d Capiotti et al (2013) Benzodiazepines in zebrafish brain hydrolysis in animals treated with 0.5 and 1 mg/L (32 and 36 %, respectively) when compared to the control group (Fig. 4).…”

Section: Resultsmentioning

confidence: 91%

Benzodiazepines alter nucleotide and nucleoside hydrolysis in zebrafish (Danio rerio) brain

et al. 2015

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Anxiety is characterized by unpleasant bodily sensations, such as pounding heart and intense fear. The therapy involves the administration of benzodiazepine drugs. Purinergic signaling participates in the induction of several behavioral patterns and their actions are inactivated by ectonucleotidases and adenosine deaminase (ADA). Since there is evidence about the involvement of purinergic system in the actions mediated by benzodiazepines, we evaluated the effects in vitro and in vivo of administration of diazepam and midazolam on nucleoside triphosphate diphosphohydrolases, ecto-5'-nucleotidase, and ADA activities in zebrafish brain, followed by the analysis of gene expression pattern of these enzymes and adenosine receptors (A1, A2a1, A2a2, A2b). The in vitro studies demonstrated that diazepam decreased ATP (66 % for 500 µM) and ADP hydrolysis (40-54 % for 10-500 µM, respectively). Midazolam decreased ATP (16-71 % for 10-500 µM, respectively) and ADP (48-73.5 % for 250-500 µM, respectively) hydrolysis as well as the ecto-ADA activity (26-27.5 % for 10-500 µM, respectively). AMP hydrolysis was decreased in animals treated with of 0.5 and 1 mg/L midazolam (32 and 36 %, respectively). Diazepam and midazolam decreased the ecto-ADA activity at 1.25 mg/L and 1 mg/L (31 and 33 %, respectively), but only 0.1 mg/L midazolam induced an increase (40 %) in cytosolic ADA. The gene expression analysis demonstrated changes on ecto-5'-nucleotidase, A1, A2a1, A2a2, and A2b mRNA transcript levels after acute treatment with benzodiazepines. These findings demonstrated that benzodiazepine exposure induces a modulation of extracellular nucleotide and nucleoside metabolism, suggesting the purinergic signaling may be, at least in part, related to benzodiazepine effects.

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“…31,33,34 Imbalance in arginine metabolism and/or availability can have repercussions on dysfunction/ disease states and affect early stages of development in mammals 34 or fish. 35 Yeast's CAR2, ARG1, ARG3, and ORT1 are also homologues of human disease-related genes. 19,37 Further studies are thus required to clarify the mechanisms underlying PYR interference with yeast arginine gene transcription and to elucidate the actual role of arginine pathway and metabolites in the ambit of PYR mode of action in yeast, mycelial fungi, and other relevant eukaryotes.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Potential Mechanisms Underlying Response to Effects of the Fungicide Pyrimethanil from Gene Expression Profiling in Saccharomyces cerevisiae

Gil¹,

Becker

Viegas³

2014

J. Agric. Food Chem.

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Pyrimethanil is a fungicide mostly applied in vineyards. When misused, residue levels detected in grape must or in the environment may be of concern. The present work aimed to analyze mechanisms underlying response to deleterious effects of pyrimethanil in the eukaryotic model Saccharomyces cerevisiae. Pyrimethanil concentration-dependent effects at phenotypic (inhibition of growth) and transcriptomic levels were examined. For transcriptional profiling, analysis focused on two sublethal exposure conditions that inhibited yeast growth by 20% or 50% compared with control cells not exposed to the fungicide. Gene expression modifications increased with the magnitude of growth inhibition, in numbers and fold-change of differentially expressed genes and in diversity of over-represented functional categories. These included mostly biosynthesis of arginine and sulfur amino acids metabolism, as well as energy conservation, antioxidant response, and multidrug transport. Several pyrimethanil-responsive genes encoded proteins sharing significant homology with proteins from phytopathogenic fungi and ecologically relevant higher eukaryotes.

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Arginine exposure alters ectonucleotidase activities and morphology of zebrafish larvae (Danio rerio)

Cited by 19 publications

References 45 publications

Acute hypoxia elevates arginase 2 and induces polyamine stress response in zebrafish via evolutionarily conserved mechanism

Acute hypoxia elevates arginase 2 and induces polyamine stress response in zebrafish via evolutionarily conserved mechanism

Benzodiazepines alter nucleotide and nucleoside hydrolysis in zebrafish (Danio rerio) brain

Potential Mechanisms Underlying Response to Effects of the Fungicide Pyrimethanil from Gene Expression Profiling in Saccharomyces cerevisiae

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