Arginine kinase shows nucleoside diphosphate kinase-like activity toward deoxythymidine diphosphate

Abstract

Arginine kinase (AK) (ATP: L-arginine phosphotransferase, E.C. 2.7.3.3) catalyzes the reversible transfer of ATP γ-phosphate group to L-arginine to synthetize phospho-arginine as a high-energy storage. Previous studies suggest additional roles for AK in cellular processes. Since AK is found only in invertebrates and it is homologous to creatine kinase from vertebrates, the objective of this work was to demonstrate nucleoside diphosphate kinase-like activity for shrimp AK. For this, AK from marine shrimp Litopenaeus vannamei (LvAK) was purified and its activity was assayed for phosphorylation of TDP using ATP as phosphate donor. Moreover, by using high-pressure liquid chromatography (HPLC) the phosphate transfer reaction was followed. Also, LvAK tryptophan fluorescence emission changes were detected by dTDP titration, suggesting that the hydrophobic environment of Trp 221, which is located in the top of the active site, is perturbed upon dTDP binding. The kinetic constants for both substrates Arg and dTDP were calculated by isothermal titration calorimetry (ITC). Besides, docking calculations suggested that dTDP could bind LvAK in the same cavity where ATP bind, and LvAK basic residues (Arg124, 126 and 309) stabilize the dTDP phosphate groups and the pyrimidine base interact with His284 and Ser122. These results suggest that LvAK bind and phosphorylate dTDP being ATP the phosphate donor, thus describing a novel alternate nucleoside diphosphate kinase-like activity for this enzyme.

Extended IgE profile of shrimp-sensitized patients based on Multiplex Examination ALEX2 – Allergy Explorer

“…This protein is found only in invertebrates, in the vertebrates, its counterpart is creatinine kinase. Arginine kinase is involved in ATP transformations and phosphoarginine synthesis [18]. Studies conducted in Italy have shown that it is a minor allergen that sensitizes 10-15% of the population of patients allergic to shrimp allergens, which is consistent with our findings [12].…”

Biochemical and structural characterization of a novel arginine kinase from the spiderPolybetes pythagoricus

“…The kinetic parameters determined for the substrate L-arginine were K m 1.7 mM and V max 27.8 μmol min −1 mg protein −1 ; within the range described for other arthropods. The horseshoe crab Limulus polyphemus ( Blethen, 1972 ), the scorpion Palamneus phipsoni ( Arjunwadkar & Raghupathi Rami Reddy, 1985 ) and tarantula Dugesiella hentzi ( Blethen & Kaplan, 1968 ) had a K m between 0.08 and 0.67 mM; in insects Cissites cephalotes ( Tanaka et al, 2007 ), Locusta migratoria ( Ilg & Werr, 2012 ; Wu et al, 2007 ), Periplaneta americana ( Brown & Grossman, 2004 ), K m was between 0.49–1.0 mM; and in the crustacean Litopenaeus vannamei ( Lopez-Zavala et al, 2016 ), Euphausia superba ( Si et al, 2014 ), the K m was 0.3 ( Table 1 ).…”

Association analysis of transcriptome and quasi-targeted metabolomics reveals the regulation mechanism underlying broiler muscle tissue development at different levels of dietary guanidinoacetic acid

“…S-methyl-L-cysteine, a sulfur-containing amino acid present in garlic, has been reported to exhibit antilipidemic activity ( 50 – 52 ). dGDP, also known as deoxythymidine diphosphate, serves as an intermediate molecule during the process of DNA synthesis ( 53 ). Adenosine, a purine nucleoside involved in both DNA and RNA synthesis, plays a crucial role in cellular growth and development.…”