-Arginine promotes capacitation and acrosome reaction in cryopreserved bovine spermatozoa

O’Flaherty, Cristián; Rodriguez, P.; Srivastava, Sudha

doi:10.1016/j.bbagen.2004.06.020

Cited by 53 publications

(43 citation statements)

References 33 publications

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“…The induction of sperm capacitation by L-Arginine at a concentration of 10 mM without affecting the viability and motility in cryopreserved bovine spermatozoa is well documented [46]. Therefore, L-Arginine favors sperm capacitation, and thus could facilitate through this process the sperm release from the oviductal epithelial cells.…”

Section: Discussionmentioning

confidence: 94%

“…Ten to 15×10 6 spermatozoa/ml were placed in 0.3% BSA sp-TALP at 38.5°C and 5% CO 2 atmosphere for 45 min [23], [46], [53] with MetAEA (1.4 nM), AEA (1 nM), L-NAME (1 µM) and Hb (30 µg/ml). Capacitation was assessed by two techniques: chlortetracycline (CTC) assay and lysophosphatidylcholine (LPC)-induced acrosome reaction assessed by Pissum sativum agglutinin-FITC staining.…”

Section: Methodsmentioning

confidence: 99%

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Anandamide Induces Sperm Release from Oviductal Epithelia through Nitric Oxide Pathway in Bovines

et al. 2012

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Mammalian spermatozoa are not able to fertilize an egg immediately upon ejaculation. They acquire this ability during their transit through the female genital tract in a process known as capacitation. The mammalian oviduct acts as a functional sperm reservoir providing a suitable environment that allows the maintenance of sperm fertilization competence until ovulation occurs. After ovulation, spermatozoa are gradually released from the oviductal reservoir in the caudal isthmus and ascend to the site of fertilization. Capacitating-related changes in sperm plasma membrane seem to be responsible for sperm release from oviductal epithelium. Anandamide is a lipid mediator that participates in the regulation of several female and male reproductive functions. Previously we have demonstrated that anandamide was capable to release spermatozoa from oviductal epithelia by induction of sperm capacitation in bovines. In the present work we studied whether anandamide might exert its effect by activating the nitric oxide (NO) pathway since this molecule has been described as a capacitating agent in spermatozoa from different species. First, we demonstrated that 1 µM NOC-18, a NO donor, and 10 mM L-Arginine, NO synthase substrate, induced the release of spermatozoa from the oviductal epithelia. Then, we observed that the anandamide effect on sperm oviduct interaction was reversed by the addition of 1 µM L-NAME, a NO synthase inhibitor, or 30 µg/ml Hemoglobin, a NO scavenger. We also demonstrated that the induction of bull sperm capacitation by nanomolar concentrations of R(+)-methanandamide or anandamide was inhibited by adding L-NAME or Hemoglobin. To study whether anandamide is able to produce NO, we measured this compound in both sperm and oviductal cells. We observed that anandamide increased the levels of NO in spermatozoa, but not in oviductal cells. These findings suggest that anandamide regulates the sperm release from oviductal epithelia probably by activating the NO pathway during sperm capacitation.

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Section: Discussionmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Anandamide Induces Sperm Release from Oviductal Epithelia through Nitric Oxide Pathway in Bovines

et al. 2012

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“…The statement is appropriate with the research 6 , which writing L-Arginine can acts as an antioxidant that protects spermatozoa from free radicals and lipid peroxidation so that the spermatozoa plasma membrane remains intact. In addition, because the dose of L-Arginine given in the medium of capacitance is to balance the occurrence of lipid peroxidation or free radicals.…”

Section: Discussionmentioning

confidence: 99%

“…According to 6 , L-Arginine is a substrate that produces nitric oxide with an intermediate enzyme nitrate oxide synthase. The enzym is in the acrosome section and the spermatozoa tail.…”

Section: Introductionmentioning

confidence: 99%

The Addition of L-Arginine in Capacitation Media to Motility, Viability, and Spermatozoa Capacity of Goats

2018

IJCTR

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:The purpose of this study is improving the provision of animal origin food of in vitro fertilization technology. Specific targets to be achieved in this study is to increase the incidence of capacitation spermatozoan and success in in vitro fertilization process . This research utilized four treatment groups, namely controlled group (P0) is the addition of LArginine in spermatozoa (HEPES) capacitance medium , group (PI) is the addition of LArginine in spermatozoa (HEPES) capacitation medium by 0,002 M / ml ,group (PII) is the addition of L-Arginine in spermatozoa (HEPES) capacitation medium by 0,004 M / ml. and group (PIII) is the addition of L-Arginine in spermatozoa (HEPES) capacitation medium by 0,006 M / ml Further evaluations included motility, viability, capacitation reaction of spermatozoa. Data obtained in the form of percentage of motility, viability and capacitation reaction is analyzed by F Test. The conclusion of this study, group (PIII) is the addition of LArginine in spermatozoa (HEPES) capacitation medium by 0,006 M / ml increase of motility, viability and capacitation reaction.

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“…Substantial evidence indicates that NO is a crucial biological messenger involved in a wide variety of physiological and pathological processes in different systems in mammals, including the vascular, nervous, and reproductive system [1,6].…”

Section: Introductionmentioning

confidence: 99%

Nitric Oxide: Key Features in Spermatozoa

Staicu¹,

Parra²

2017

Nitric Oxide Synthase - Simple Enzyme-Complex Roles

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Several in vitro studies have pointed to the importance of nitric oxide (NO) in the female and male reproductive system in mammals. Its functions vary from preventing oocyte aging, improving the integrity of the microtubular spindle apparatus in aged oocytes, modulating the contraction of the oviduct, to regulating sperm physiology by affecting the motility, inducing chemotaxis in spermatozoa, regulating tyrosine phosphorylation, enhancing the sperm-zona pellucida binding ability, and modulating the acrosomal reaction. In spermatozoa, NO exerts its functions in different ways, which involve key elements such as the soluble isoform of guanylate cyclase, cyclic guanosine monophosphate (cGMP), cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), adenylate cyclase, and the extracellular signal-regulated kinase (ERK) pathway. Furthermore, NO leads to the S-nitrosylation of several sperm proteins, among them a substantial group associated with energy generation and cell movement, but also with signal transduction, suggesting a role for S-nitrosylation in sperm motility and in modulating the sperm function, respectively. In this chapter, an overview of how NO modulates the sperm physiology is presented, based on the knowledge acquired to this day.

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-Arginine promotes capacitation and acrosome reaction in cryopreserved bovine spermatozoa

Cited by 53 publications

References 33 publications

Anandamide Induces Sperm Release from Oviductal Epithelia through Nitric Oxide Pathway in Bovines

Anandamide Induces Sperm Release from Oviductal Epithelia through Nitric Oxide Pathway in Bovines

The Addition of L-Arginine in Capacitation Media to Motility, Viability, and Spermatozoa Capacity of Goats

Nitric Oxide: Key Features in Spermatozoa

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