Argon detector: alternative detection system for gas-liquid chromatographic analysis of short-chain organic acids

Résumé -Bien qu'intrinsèquement non toxiques, les gaz nobles comme l'argon entraînent à fortes concentrations une déplétion d'oxygène pouvant mener jusqu'à l'asphyxie. Si l'argon se retrouve dans la circulation générale, celui-ci peut même être responsable d'embolies parfois fatales. Cependant, les propriétés d'inerties physicochimiques de ce gaz le rendent très utile dans de nombreuses applications actuelles. Ces applications étant en constante augmentation, il n'est pas rare d'observer proportionnellement des intoxications parfois létales liées à ce gaz. Ainsi, devant le manque d'harmonisation des prélèvements d'échantillons biologiques afin de mettre en évidence de telles intoxications aux gaz nobles et devant l'augmentation du nombre de cas, il devient nécessaire d'établir un état des lieux sur l'utilisation de l'argon, sa toxicité et la stratégie analytique à adopter dans le contexte médico-légal. Mots clés :Argon, gaz noble, échantillonnage de gaz, analyse de gaz Abstract -Even though noble gases such as argon are intrisically non-toxic, they will enhance an oxygen-depletion at high concentrations. This depletion may lead to asphyxia. If argon is found in blood flow, it could be responsible of an embolism. However, its inert physicochemical properties appear to be very useful to a wide range of current applications. The rise of these applications induces a proportionnal increase of lethal intoxications due to this gas. Thus, as no standardization of biological samples exists, and knowing the increase of argon intoxication cases, it seems necessary to establish an incoming inventory of fixture concerning the use of argon. It will confirm its toxicity properties and will highlight other intoxications due to noble gas. This should lead to a new analytical strategy suitable to the forensic context.

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Comparison of traditional gas chromatography (GC), headspace GC, and the microbial identification library GC system for the identification of Clostridium difficile

Cundy¹,

Willard²,

Valeri³

et al. 1991

J Clin Microbiol

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Three gas chromatography (GC) methods were compared for the identification of 52 clinical Clostridium difficile isolates, as well as 17 non-C. difficile Clostridium isolates. Headspace GC and Microbial Identification System (MIS) GC, an automated system which utilizes a software library developed at the Virginia Polytechnic Institute to identify organisms based on the fatty acids extracted from the bacterial cell wall, were compared against the reference method of traditional GC. Headspace GC and MIS were of approximately equivalent accuracy in identifying the 52 C. difficile isolates (52 of 52 versus 51 of 52, respectively). However, 7 of 52 organisms required repeated sample preparation before an identification was achieved by the MIS method. Both systems effectively differentiated C. difficile from non-C. difficile clostridia, although the MIS method correctly identified only 9 of 17. We conclude that the headspace GC system is an accurate method of C. difficile identification, which requires only one-fifth of the sample preparation time of MIS GC and one-half of the sample preparation time of traditional GC.

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Argon detector: alternative detection system for gas-liquid chromatographic analysis of short-chain organic acids

Cited by 3 publications

References 6 publications

Standard and Experimental Methods of Identification and Enumeration

Standard and Experimental Methods of Identification and Enumeration

L’argon : utilisations, toxicité et stratégie analytique en toxicologie médicolégale

Comparison of traditional gas chromatography (GC), headspace GC, and the microbial identification library GC system for the identification of Clostridium difficile

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