The main purpose of this study is to investigate prospective teachers' critical thinking and metacognition levels. The study is descriptive in nature and based on relational screening model. The participants were selected using stratified sampling method which is one of the random sampling methods, and 293 teachers became the participants of the study. The data collected via "California Critical Thinking Scale" and "Metacognition Scale" were analyzed using independent groups t-test, one-way variance analysis, correlation analysis as well as the descriptive statistics. Results show that although the participants' metacognition perceptions differed according to gender in the evaluation and organization dimensions, no significant differences were found in the other dimensions. The participants' perceived critical thinking levels did not show significant differences according to the departments they attend in the seeking truth, open-mindedness, analyticalness, systematicity, curiousness and total dimensions. Critical thinking total scores were found to display a negative relationship with evaluation, organization, and metacognition total scores while they showed a positive relationship with seeking the truth, open-mindedness, analyticalness, systematicity, self-confidence, and curiousness scores.