Argyrophilic nucleolar organiser region counts and prognosis in pharyngeal carcinoma

Pich, Achille; Pisani, Paola; Kzengli, M; Cappello, N.; Navone, Roberto

doi:10.1038/bjc.1991.300

Cited by 18 publications

(4 citation statements)

References 46 publications

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“…For AgNOR staining and counting 3 gm thick sections taken to an aqueous medium were stained with the AgNOR method of Ploton et al (1986), as previously described (Pich et al 1991). Random fields, excluding areas of necrosis, were independently examined by two pathologists (AP and LC) using a 100 x oil immersion lens; at least 100 tumour cells were counted in each case.…”

Section: Methodsmentioning

confidence: 99%

Cell proliferation indices, morphometry and DNA flow cytometry provide objective criteria for distinguishing low and high grade bladder carcinomas

Pich

Chiusa

Comino

et al. 1994

Vichows Archiv A Pathol Anat

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Argyrophilic nucleolar organizer region (Ag-NOR) analysis, proliferating cell nuclear antigen (PC-NA/PC10) and MIB-1 immunohistochemistry, nuclear morphometry and DNA flow cytometry have been performed on formalin-fixed, paraffin-embedded biopsies from 50 patients with transitional cell carcinoma of the urinary bladder. The mean AgNOR count was 6.01 for the 17 grade 1 (G1), 7.59 for the 21 G2 and 13.33 for the 12 G3 carcinomas (p < 0.001). The mean PCNA score was 15.03% for G1, 24.04% for G2 and 40.01% for G3 cases (p < 0.001). The mean MIB-1 score was 11.31% for G1, 17.09% for G2 and 34.47% for G3 carcinomas (p < 0.001). The mean nuclear area was 35.53 microns2 for G1, 38.65 microns2 for G2 and 83.62 microns2 for G3 cases (p < 0.001). Aneuploidy rates were significantly higher (91.7%) in G3 than in G2 (42.9%, p < 0.01) or G1 cases (47.1%, p < 0.05) but not different for G1 versus G2 cases (p = 0.94). While many overlaps of values were seen between G1 and G2 tumours, no overlaps were found between G3 and G1/G2 tumours. Significant differences of values were also found between pTa and invasive tumours (p < 0.0001 for AgNOR count and PCNA score; p < 0.001 for MIB-1 score and mean nuclear area; p < 0.01 for DNA ploidy); however many overlaps were seen. Our findings indicate that the quantitative parameters obtained with different methods are associated with histological grade of bladder urotheliomas and may improve the grading reproducibility. In addition, the absence of overlaps between G3 and G2/G1 carcinomas supports the tendency to classify bladder urotheliomas in only two categories of malignancy.

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Section: Methodsmentioning

confidence: 99%

Cell proliferation indices, morphometry and DNA flow cytometry provide objective criteria for distinguishing low and high grade bladder carcinomas

Pich

Chiusa

Comino

et al. 1994

Vichows Archiv A Pathol Anat

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“…AgNOR staining was performed according to Ploton et al [37] as previously described [33]. AgNORs were counted according to Crocker et al [9], and the mean number per nucleus was calculated in each case.…”

Section: Introductionmentioning

confidence: 99%

Bcl-2 expression in male breast carcinoma

Pich¹,

Margaria²,

Chiusa³

1998

Virchows Archiv

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We have analysed the expression of bcl-2 protein retrospectively in 34 primary male breast carcinomas (MBC), using the monoclonal antibody bcl-2 in formalin-fixed, paraffin-embedded tissues. Bcl-2 expression was compared with tumour clinicopathological features, sex steroid hormone receptors, DNA content, p53 immunoreactivity and cell proliferative activity assessed by counts of the argyrophilic nucleolar organizer regions (AgNORs), the monoclonal antibody PC10 against proliferating cell nuclear antigen and the monoclonal antibody MIB-1. Most (28, or 82.3%) of the 34 cases of MBC were bcl-2 positive. No association was found with clinicopathological features of the tumours, although bcl-2 tended to be more frequently expressed in small tumours (P=0.09) and in cases without necrotic areas (P=0.1). Nor was any association found with hormone receptor status, p53 immunoreactivity, DNA content, cell proliferative activity or patient survival. In multivariate analysis, only proliferative activity (expressed by AgNOR counts) and p53 immunoreactivity had independent prognostic significance. Our results indicate that MBC differs from FBC in that in MBC bcl-2 protein is not related to an oestrogen-dependent transcription pathway and bcl-2 alone is not sufficient to induce increased proliferation. These characteristics, together with the high prognostic value of cell proliferation and the lack of prognostic significance for hormone receptor status, support the hypothesis that MBC is biologically different from FBC.

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“…Previous studies concerning the prognostic significance of AgNOR counts in squamous cell carcinomas of the head and neck are in accordance with our findings and show that high values for mean AgNOR counts concur with poor prognosis. [16][17][18][19][20] Two of these studies 17,19 applied multivariate analysis and both showed that mean AgNOR counts was the strongest single parameter in separating prognostically favorable and unfavorable cases. Contrary to Pich et al 17 and Bochmü hl et al 19 studying pharyngeal and laryngeal carcinomas, we found that T1-T2 carcinomas had significantly lower mean AgNOR counts than did T3-T4 tumors.…”

Section: Discussionmentioning

confidence: 99%

“…The N classification did not influence the mean AgNOR counts. 17,19 The mean number of AgNORs per tumor nucleus is the method employed by most investigators. Previous studies on head and neck squamous cell carcinomas revealed an overall mean AgNOR count ranging from 4.3 to 10.7.…”

Section: Discussionmentioning

confidence: 99%

Nucleolar organizer regions and prognosis in glottic squamous cell carcinoma

et al. 1997

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Different parameters of fibrinolytic systems like t‐PA, PAI, D‐dimer, and inhibitors of blood coagulation, i.e., protein C (PC), protein S(PS), and antithrombin III (AT‐III), have been studied in cases of acute malaria due to Plasmodium falciparum and plasmodium vivax infection, and these patients were followed up. It was observed that the plasma PAI‐1 was very high in cases of P. falciparum malaria infection as compared to normal controls and P. vivax infection. The changes in complicated cases of P. falciparum were remarkable as compared to uncomplicated ones. The PC, PS, and AT‐III levels were also low in P. falciparum, particularly so in complicated cases, and were normal in P. vivax infection. The factor VIII R:Ag levels were invariably high in acute malaria. On follow‐up of some of these cases the values came back to normal after the antiparasite treatment. The monocyte procoagulant activity was found to be significantly higher in P. falciparum infection as compared to that of P. vivax infection. All these findings therefore contribute towards the production of a hypercoagulable state in P. falciparum infection and partly explain the complications of P. falciparum infection like cerebral malaria. Am. J. Hematol. 54:23–29, 1997 © 1997 Wiley‐Liss, Inc.

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Argyrophilic nucleolar organiser region counts and prognosis in pharyngeal carcinoma

Abstract: Summary The prognostic significance of argyrophilic nucleolar organiser regions (AgNORs)

Cited by 18 publications

References 46 publications

Cell proliferation indices, morphometry and DNA flow cytometry provide objective criteria for distinguishing low and high grade bladder carcinomas

Cell proliferation indices, morphometry and DNA flow cytometry provide objective criteria for distinguishing low and high grade bladder carcinomas

Bcl-2 expression in male breast carcinoma

Nucleolar organizer regions and prognosis in glottic squamous cell carcinoma

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