Armazenamento de sementes e aclimatização de Brassavola tuberculata Hook

Macedo, Marichel Canazza de; Rosa, Derek Brito Chaim Jardim; Soares, Jackeline Schultz; Tatara, Mariana Bento; Hoffmann, Nilda Tiyoko Kobayashi; Rosa, Yara Brito Chaim Jardim

doi:10.5433/1679-0359.2014v35n6p2883

Cited by 8 publications

(13 citation statements)

References 13 publications

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“…The TTC test have been used in various orchid studies but the results have been variable and inconsistent even within species (Alvarez-Pardo et al 2006 ; Custódio et al 2016 ; de Macedo et al 2014 ; Dowling and Jusaitis 2012 ; Hosomi et al 2011 , 2012 ; Lallana and García 2013 ; Soares et al 2014 ; Vujanovic et al 2000 ; Yamazaki and Miyoshi 2006 ). The instability with the TTC test was seen in this study too.…”

Section: Discussionmentioning

confidence: 99%

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Seed viability testing for research and conservation of epiphytic and terrestrial orchids

et al. 2022

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Background Seed viability testing is essential in plant conservation and research. Seed viability testing determines the success of ex-situ conservation efforts, such as seed banking but commonly testing protocols of orchids lack consistency and accuracy, therefore, there is a need to select an appropriate and reliable viability test, especially when conducting comparative studies. Here, we evaluated the suitability of three seed viability tests, Evans blue test (EB), Fluorescein diacetate test (FDA) and Tetrazolium test (TTC), with and without sterilization, on seeds of 20 orchid species, which included five epiphytes and fifteen terrestrials, using both fresh seeds and seeds stored at − 18 ºC for 6 to 8 years. Results We found that sterilization and lifeform of seeds affected seed viability across all tests but the storage time was not an influential factor. Sterilization negatively affected seed viability under EB and FDA test conditions but increased the detection of viable seeds in the TTC test in both epiphytic and terrestrial species. The EB test, when administered without sterilization provided the highest viability results. Being non-enzymatic unlike TTC and FDA tests, as expected, the EB test was the most reliable with similar results between sterilized and not sterilized seeds for most epiphytic and terrestrial species as well as when compared between groups. Conclusions The lifeform of the species and seed sterilization prior to testing are important influential factors in orchid seed viability testing. Since EB test was found to be reliable we recommend the EB test for seed viability assessment in orchids rather than the less reliable but commonly used TTC test, or the FDA test, which require more expensive and sophisticated instrumentation. Since storage time was not an influential factor in orchid seed viability testing, the recommendations of this study can be used for both fresh as well as long-term stored orchid seeds. This is helpful for research and especially for conservation measures such as seed banking. However, due to the species specificity of the bio-physiology of orchids, we call for comprehensive viability test assessment in the hyper diverse orchid family to be extended to a greater number of species to facilitate efficient conservation and research.

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Section: Discussionmentioning

confidence: 99%

“…This is especially important for species that are slow and hard to germinate. However, it can be difficult to interpret, requiring experience to determine the test results correctly and results can be inconsistent even within the same seed lot, or within a species (Custódio et al 2016 ; de Macedo et al 2014 ; Soares et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Seed viability testing for research and conservation of epiphytic and terrestrial orchids

et al. 2022

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“…Thus, in relation to seed storage and conservation, orchids are similar to species that have orthodox seeds, since the longevity of the seeds is increased by reducing the moisture degree (from about 20% wet basis to 5%) and decreasing storage temperatures from 62 °C to 0 °C (PRITCHARD & SEATON, 1993;MACEDO et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Degree of moisture in seeds for the cryopreservation of orchids native to Brazil

et al. 2020

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The objective of this study was to determine the ideal moisture degree for the cryopreservation of Cattleya labiata Lindl. and Miltonia regnellii Rchb.f. orchid seeds. Orchid seeds were standardized in relation to the desired moisture degrees (4, 6, 8, 10, 12, and 15%) and were subjected to rapid freezing in liquid nitrogen (-196 °C) for 24 h; then, the seeds were defrosted and analyzed. For both orchid species, the seeds that were immersed in liquid nitrogen with 4% moisture content had the highest viability percentage, averaging 95% and 68% of viable seeds for C. labiata and M. regnellii, respectively. The seeds with 12% and 15% moisture content had no viable seeds. For the fresh mass of seedlings, there was no significant difference between moisture content treatments for either species.

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“…Methodologies for the tetrazolium test in Orchidaceae seeds have been proposed in the literature (Hosomi et al 2011, Hosomi et al 2012, Hosomi et al 2017, Macedo et al 2014. However, changes in the seed pre-conditioning and conditioning procedures for the test may increase the seed efficiency, since these changes aim to improve the visualization of viable seeds (Salazar & Gélvez 2015, Custódio et al 2016, Seaton et al 2018, Mercado et al 2020.…”

Section: Special Supplement: Cerrado [Brazilian Savanna]mentioning

confidence: 99%

Seed viability test of orchids native to the Brazilian Savanna1

Soares

Sorgato

Ribeiro

et al. 2021

Pesqui. Agropecu. Trop.

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An essential factor for the formation of a native orchid seed bank is the identification of the viability of stored seeds. This study aimed to determine a methodology for optimizing the tetrazolium test, in the identification of the viability of stored seeds of two Orchidaceae medicinal species native to the Brazilian Savanna. Seeds of Miltonia flavescens Lindl. and Schomburgkia crispa Lindl. were submitted to three pre-conditioning conditions: no soaking (control), sucrose solution or distilled water. The seeds were then submitted to a tetrazolium solution, with three types of conditioning: oven, water bath or ambient temperature. The M. flavescens seeds showed a germination of 86.91 %, with a higher percentage of viable seeds (62.32 %) when submitted to pre-conditioning with sucrose + oven conditioning. For S. crispa, the germination was 97.78 %, with a higher percentage of viable seeds for the control treatment + ambient temperature (89.49 %). These results suggest that specific protocols should be used to conduct the tetrazolium test in Orchidaceae. Moreover, when performed on a sample basis, the tetrazolium test should only be used to indicate the seed viability.

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Armazenamento de sementes e aclimatização de Brassavola tuberculata Hook

Cited by 8 publications

References 13 publications

Seed viability testing for research and conservation of epiphytic and terrestrial orchids

Seed viability testing for research and conservation of epiphytic and terrestrial orchids

Degree of moisture in seeds for the cryopreservation of orchids native to Brazil

Seed viability test of orchids native to the Brazilian Savanna1

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