Aromatase in testis: Expression and role in male reproduction

Lambard, Sophie; Silandre, Dorothèe; Delalande, Christelle; Denis-Galeraud, Isabelle; Bourguiba, Sonia; Carreau, Serge

doi:10.1016/j.jsbmb.2005.04.020

Cited by 62 publications

(43 citation statements)

References 58 publications

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“…In the rat, the expression of aromatase is threefold higher in pachytene spermatocytes compared with gonocytes (15). In the prepubertal human male, we report active expression of both aromatase and ER␤ in GCs, indicating that estrogens can be synthesized in GCs from androgens provided by LCs and precursor ICs, to have an effect in the same GCs and/or in neighboring SCs.…”

Section: Sex Hormone Receptors In Immature Testismentioning

confidence: 67%

“…Buenos Aires, Argentina) following the manufacturer's instructions. Briefly, 5 g of total RNA and 500 ng of oligo (dT) 15 primer (Biodynamics SRL, Buenos Aires, Argentina) were denatured by heating to 70% for 10 min, quickly chilled on ice, and subsequently incubated with 200 U of MuMLV-RT, 2.5 L of fivefold concentrated RT reaction buffer, 1 mmol/L of each deoxyribonucleoside triphosphate (dNTP) (Promega, Buenos Aires, Argentina) and 25 U of porcine RNAguard Ribonuclease Inhibitor (Amersham Biosciences) in a 25 L of reaction volume, at 37°C for 60 min.…”

Section: Methodsmentioning

confidence: 99%

“…In the adult human, testis aromatase is localized to LCs and GCs (15). We have reported that aromatase mRNA is expressed in the prepubertal human testis, including the period of early postnatal activation (16), when local testosterone substrate production is high.…”

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confidence: 99%

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Expression of Aromatase, Estrogen Receptor α and β, Androgen Receptor, and Cytochrome P-450scc in the Human Early Prepubertal Testis

et al. 2006

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ABSTRACT:The expression of aromatase, estrogen receptor ␣ (ER␣) and ␤ (ER␤), androgen receptor (AR), and cytochrome P-450 side chain cleavage enzyme (cP450 scc ) was studied in prepubertal testis. Samples were divided in three age groups (GRs): GR1, newborns (1-to 21-d-old neonates, n ϭ 5); GR2, postnatal activation stage (1-to 7-mo-old infants, n ϭ 6); GR3, childhood (12-to 60-mo-old boys, n ϭ 4). Absent or very poor detection of ER␣ by immunohistochemistry in all cells and by mRNA expression was observed. Leydig cells (LCs) of GR1 and GR2 showed strong immunostaining of aromatase and cP450 scc but weak staining of ER␤ and AR. Interstitial cells (ICs) and Sertoli cells (SCs) expressed ER␤, particularly in GR1 and GR2. Strong expression of AR was found in peritubular cells (PCs). For all markers, expression in GR3 was the weakest. In germ cells (GCs), i.e. gonocytes and spermatogonia, aromatase and ER␤ were immunoexpressed strongly whereas no expression of ER␣, AR, or cP450 scc was detected. It is proposed that in newborn and infantile testis, testosterone acting on PCs might modulate infant LC differentiation, whereas the absence of AR in SCs prevents development of spermatogenesis. The role of estrogen is less clear, but it could modulate the preservation of an adequate pool of precursor LCs and GCs. (Pediatr Res 60: 740-744, 2006)

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Section: Sex Hormone Receptors In Immature Testismentioning

confidence: 67%

Section: Methodsmentioning

confidence: 99%

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Expression of Aromatase, Estrogen Receptor α and β, Androgen Receptor, and Cytochrome P-450scc in the Human Early Prepubertal Testis

et al. 2006

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“…Such a receptor blockade prevents estrogen receptor activation, mimicking the aromatase gene knockout (ArKO) phenotype characterized by abnormally low estrogen biosynthesis. ArKO animals have abnormal seminiferous tubule morphology, defects in spermatogenesis, an increased apoptosis rate, and reduced fertility, and ArKO animals exhibit decreased mating behavior (Akingbemi 2005;Lambard et al 2005). The increased space observed between the seminiferous tubules in bats treated with COU could be due to phytoestrogen antagonism of estrogen receptors interfering with endogenous estrogen signaling, and thereby affecting fluid absorption in the afferent conducts and seminiferous tubules.…”

Section: Resultsmentioning

confidence: 99%

Histometric analysis of vampire bat (Desmodus rotundus) testicles treated with coumestrol by oral route

Pérez-Rivero

Pérez-Martínez

Aguilar-Setién

2013

Journal of Applied Animal Research

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The vampire bat (Desmodus rotundus) is a hematophagous species, and is considered the main transmitter of rabies to cattle in Latin America. The purpose of the present study was to evaluate the effect of coumestrol (COU; 5 mg per g body weight, administered orally for 30 d) on the histoarchitecture of vampire bat testicles. A total of 10 male vampire bats were divided randomly into two groups: COU-treated and control (N 05 per group). Testis samples were obtained for histometric evaluation. The numbers of epididymal sections containing spermatozoa for each bat were counted by light microscopy and fluorescent signal detection was performed by confocal microscopy. We found significant group differences in germinal epithelium thickness, seminiferous tube diameter, intertubular space, number of spermatogenia, Sertoli cell counts, spermatid cell counts, Leydig cell counts, and the number of epididymal sections with spermatozoa. Based on our results, we conclude that COU, when administered orally for 30 d, can modify the histoarchitecture of vampire bat testicular tissue in a manner that would be expected to impair fertility.

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“…More recent studies have revealed LRH-1 expression in the adrenal gland, adipocytes, pancreas, and gut (Sirianni et al 2002. Especially, high levels of LRH-1 mRNA are present in the ovary and testis (Falender et al 2003, Hinshelwood et al 2003, Lambard et al 2005. LRH-1, like SF-1, has been demonstrated to stimulate expression of genes which encode steroidogenic enzymes, including the StAR, CYP11A1 and the aromatase genes (Sirianni et al 2002.…”

Section: Introductionmentioning

confidence: 99%

Liver receptor homologue-1 regulates gonadotrope function

Zheng¹,

Yang²,

Jiang³

et al. 2007

Journal of Molecular Endocrinology

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Over the past decade, substantial advances have been made in our understanding of the transcription factors which regulate gene expression in gonadotropes. One of the most important of these factors, steroidogenic factor-1 (SF-1; NR5A1) is critical for gonadotropin and GnRH-receptor expression. Interestingly, a closely related nuclear hormone receptor, liver receptor homologue-1 (LRH-1; NR5A2) has recently been detected in the anterior pituitary gland; however, its functional significance in this tissue has not been investigated. For the experiments reported here, we hypothesized that LRH-1 plays a previously unrecognized role in gonadotrope physiology. Towards this end, we first demonstrate LRH-1 mRNA and protein expression in both primary pituitary cells and gonadotrope-derived cell lines. We next show that LRH-1 stimulates promoter activity of the GnRH-receptor and gonadotropin subunit genes. Within the LHb gene, this response appears to be mediated by DNA-binding and transactivation through previously characterized SF-1 ciselements. To our knowledge, this is the first report demonstrating a functional role for LRH-1 in the gonadotrope population of the anterior pituitary gland.

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Aromatase in testis: Expression and role in male reproduction

Cited by 62 publications

References 58 publications

Expression of Aromatase, Estrogen Receptor α and β, Androgen Receptor, and Cytochrome P-450scc in the Human Early Prepubertal Testis

Expression of Aromatase, Estrogen Receptor α and β, Androgen Receptor, and Cytochrome P-450scc in the Human Early Prepubertal Testis

Histometric analysis of vampire bat (Desmodus rotundus) testicles treated with coumestrol by oral route

Liver receptor homologue-1 regulates gonadotrope function

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