Arp2/3-dependent mechanical control of morphogenetic robustness in an inherently challenging environment

Martin, Emmanuel; Theis, Sophie; Gay, G.; Monier, Brice; Rouvière, Christian; Suzanne, Magali

doi:10.1016/j.devcel.2021.01.005

Cited by 25 publications

(16 citation statements)

References 52 publications

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“…So it is possible that mechanosensitive feedback increase actomyosin enrichment along PSBs, contributing to the real-time responsiveness of boundary formation. This might also contribute to the robustness of boundary formation (Martin et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Different temporal requirements for the LRR transmembrane receptors Tartan and Toll-2 in the formation of contractile interfaces at compartmental boundaries

Sharrock

Blanchard

Evans

et al. 2021

Preprint

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Compartmental boundaries physically separate groups of epithelial cells, a property fundamental for the organization of the body plan in both insects and vertebrates. In many examples, this physical separation has been shown to be the consequence of a regulated increase in contractility of the actomyosin cortex at boundary cell-cell interfaces, a property important in developmental morphogenesis beyond compartmental boundary formation. In this study, we took an unbiased screening approach to identify cell surface receptors required for actomyosin enrichment and polarisation at parasegmental boundaries (PSBs) in early Drosophila embryos, leading us to uncover different temporal requirements for two LRR receptors, Tartan and Toll-2. First, we find that Tartan is required during germband extension for actomyosin enrichment at PSBs, confirming an earlier report. Next, by following in real time the dynamics of loss of boundary straightness in tartan mutant embryos compared to wildtype and ftz mutant embryos, we show that Tartan is not required beyond germband extension. At this stage, actomyosin enrichment at PSBs becomes regulated by Wingless signalling. We find that Wingless signalling regulates Toll-2 expression and we show that Toll-2 is required for planar polarization of actomyosin after the completion of germ-band extension. Thus the formation of contractile interfaces at PSBs depends on a dynamic set of LRR receptors cues. Our study also suggests that the number of receptor cues is small and that the receptors are interchangeable.

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Section: Discussionmentioning

confidence: 99%

Different temporal requirements for the LRR transmembrane receptors Tartan and Toll-2 in the formation of contractile interfaces at compartmental boundaries

Sharrock

Blanchard

Evans

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Several recent works demonstrate that tissue-scale patterns and coupling of the actomyosin cytoskeleton provide robustness during tissue folding [ 69 , 70 , 71 ]. In particular, during leg folding, myosin II is planar polarised at adherens junctions, orienting force propagation along the fold axis.…”

Section: The Nucleus From An Organelle Under Force To An Organelle Necessary To Generate Morphogenetic Forcesmentioning

confidence: 99%

Orchestration of Force Generation and Nuclear Collapse in Apoptotic Cells

Monier

2021

IJMS

Self Cite

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Apoptosis, or programmed cell death, is a form of cell suicide that is extremely important for ridding the body of cells that are no longer required, to protect the body against hazardous cells, such as cancerous ones, and to promote tissue morphogenesis during animal development. Upon reception of a death stimulus, the doomed cell activates biochemical pathways that eventually converge on the activation of dedicated enzymes, caspases. Numerous pieces of information on the biochemical control of the process have been gathered, from the successive events of caspase activation to the identification of their targets, such as lamins, which constitute the nuclear skeleton. Yet, evidence from multiple systems now shows that apoptosis is also a mechanical process, which may even ultimately impinge on the morphogenesis of the surrounding tissues. This mechanical role relies on dramatic actomyosin cytoskeleton remodelling, and on its coupling with the nucleus before nucleus fragmentation. Here, we provide an overview of apoptosis before describing how apoptotic forces could combine with selective caspase-dependent proteolysis to orchestrate nucleus destruction.

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“…In the Drosophila leg disc, a precise pattern of four parallel folds forms during development and perturbations of Arp2/3 complex component were found to induce deviated folds [137] . Planar polarization of MyoII was shown to be necessary to render the folds insensitive to mechanical perturbation: without it, folds initiate properly but propagate with low precision due to local mechanical noise (e.g.…”

Section: Developmental Robustnessmentioning

confidence: 99%

“…Planar polarization of MyoII was shown to be necessary to render the folds insensitive to mechanical perturbation: without it, folds initiate properly but propagate with low precision due to local mechanical noise (e.g. they deviate towards regions of high tension) [137] . The polarization of MyoII ensures that force transmission is biased in the direction of future fold formation, “buffering” the effect of additional forces in the tissue.…”

Section: Developmental Robustnessmentioning

confidence: 99%

Forced into shape: Mechanical forces in Drosophila development and homeostasis

Paci

Mao

2021

Seminars in Cell & Developmental Biology

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Mechanical forces play a central role in shaping tissues during development and maintaining epithelial integrity in homeostasis. In this review, we discuss the roles of mechanical forces in Drosophila development and homeostasis, starting from the interplay of mechanics with cell growth and division. We then discuss several examples of morphogenetic processes where complex 3D structures are shaped by mechanical forces, followed by a closer look at patterning processes. We also review the role of forces in homeostatic processes, including cell elimination and wound healing. Finally, we look at the interplay of mechanics and developmental robustness and discuss open questions in the field, as well as novel approaches that will help tackle them in the future.

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Arp2/3-dependent mechanical control of morphogenetic robustness in an inherently challenging environment

Cited by 25 publications

References 52 publications

Different temporal requirements for the LRR transmembrane receptors Tartan and Toll-2 in the formation of contractile interfaces at compartmental boundaries

Different temporal requirements for the LRR transmembrane receptors Tartan and Toll-2 in the formation of contractile interfaces at compartmental boundaries

Orchestration of Force Generation and Nuclear Collapse in Apoptotic Cells

Forced into shape: Mechanical forces in Drosophila development and homeostasis

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