2004
DOI: 10.1074/jbc.m402357200
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ARPC1/Arc40 Mediates the Interaction of the Actin-related Protein 2 and 3 Complex with Wiskott-Aldrich Syndrome Protein Family Activators

Abstract: The actin-related protein 2 and 3 (Arp2/3) complex is a seven-subunit protein complex that nucleates actin filaments at the cell cortex. Despite extensive cross-linking, crystallography, genetic and biochemical studies, the contribution of each subunit to the activity of the complex remains largely unclear. In this study we characterized the function of the 40-kDa subunit, ARPC1/ Arc40, of the yeast Arp2/3 complex. We showed that this subunit is indeed a stable component of the Arp2/3 complex, but its highly u… Show more

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Cited by 46 publications
(58 citation statements)
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“…This conclusion is in agreement with other studies indicating that the high-affinity site is on Arp3 (48). However, some experiments point to the Arp2-ARPC1 site as the high-affinity site (26,27,33). For instance, a recent ITC study directly demonstrated that WASP delivers actin monomers to the Arp2 site first, suggesting that, at least in the presence of monomers, the Arp2 site is the higher-affinity site (49).…”
Section: Discussionsupporting
confidence: 79%
See 1 more Smart Citation
“…This conclusion is in agreement with other studies indicating that the high-affinity site is on Arp3 (48). However, some experiments point to the Arp2-ARPC1 site as the high-affinity site (26,27,33). For instance, a recent ITC study directly demonstrated that WASP delivers actin monomers to the Arp2 site first, suggesting that, at least in the presence of monomers, the Arp2 site is the higher-affinity site (49).…”
Section: Discussionsupporting
confidence: 79%
“…Less is known about interactions with A, but a short segment of electron density between subdomains 3 and 4 in Arp3 in a cocrystal structure was interpreted as the conserved tryptophan from the A region of one bound WASP (27). The second A region is thought to bind the ARPC1 subunit, because this subunit in isolation can bind to VCA in a mode dependent on the conserved tryptophan (33). These data, together with distance measurements reported in a recent FRET study (26), have led to an approximate model for CA binding at each site (Fig.…”
mentioning
confidence: 99%
“…These repeats are found in many signaling proteins and mediate numerous types of protein-protein interactions, particularly with highly conserved disordered regions. Previously it was shown that within the yeast Arp2/3 complex, the p40 subunit provides most of the binding energy for the CA peptide, and isolated p40 binds VCA with similar affinity to the intact complex (34). These results are not entirely consistent with previous cross-linking studies nor with our data showing that the C domain provides little binding energy for the Arp2/3 complex.…”
Section: Discussioncontrasting
confidence: 54%
“…The ability to make mutant yeast actins and mutant Arp2/3 complex (20,31,36) subunits in yeast presents a way that one might use to delineate the mechanism by which yeast actin exerts this specific effect on the yeast Arp2/3 complex.…”
Section: Discussionmentioning
confidence: 99%