ARPC2 promotes breast cancer proliferation and metastasis

Cheng, Zhongle; Wei, Wei; Wu, Zhengshen; Wang, Jing; Ding, Xiaofeng; Sheng, Yucheng; Han, Yinli; Wu, Qiang

doi:10.3892/or.2019.7113

Cited by 27 publications

(31 citation statements)

References 40 publications

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“…AS014; 1:3,000; ABclonal Biotechnology Co., Ltd.) for 1 h at room temperature. The protein bands were visualized using an ECL reagent (EMD Millipore) and analyzed using the Tanon 5200 image acquisition system (Tanon Science and Technology Co., Ltd.), as previously described (30). The band intensity of western blotting was measured by densitometry using Image-Pro Plus J software (version 6.0; National Institutes of Health).…”

Section: Methodsmentioning

confidence: 99%

Tanshinone IIA reverses EGF‑ and TGF‑β1‑mediated epithelial‑mesenchymal transition in HepG2 cells via the PI3K/Akt/ERK signaling pathway

Zhang¹,

Lin²,

Chen³

et al. 2019

Oncol Lett

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Epithelial-to-mesenchymal transition (EMT) is an essential phenotypic conversion involved in cancer progression. Epidermal growth factor (EGF) and transforming growth factor (TGF)-β1 are potent inducers of the EMT. Tanshinone IIA (Tan IIA) is a phenanthrenequinone extracted from the root of Salvia miltiorrhiza Bunge, and its anticancer activity has been demonstrated in numerous studies. However, the mechanisms of action underlying Tan IIA in EGF-and TGF-β1-induced EMT in HepG2 cells remain unknown. Multiple assays were utilized in the present study, including colony formation, wound healing, Transwell invasion, immunofluorescence staining and western blotting, in order to assess the influence of Tan IIA on HepG2 cells induced by 20 ng/ml EGF and 10 ng/ml TGF-β1. The present study reported that Tan IIA treatment decreased EGF-and TGF-β1-enhanced cell colony numbers, migration and invasion, and inhibited EGF-and TGF-β1-induced decreases in the expression levels of E-cadherin, and increases in the expression levels of matrix metalloproteinase-2, N-cadherin, vimentin and Snail. In addition, it was observed that Tan IIA decreased the expression levels of phosphorylated (p)-Akt and p-ERK1/2 induced by EGF and TGF-β1. Furthermore, western blot analysis confirmed that blocking the function of PI3K/Akt and ERK with LY294002 and U0126 resulted in upregulation of E-cadherin expression, and downregulation of vimentin and Snail expression in EGF-and TGF-β1-treated HepG2 cells. In conclusion, to the best of our knowledge, the results of the present study are the first to indicate that Tan IIA may suppress EGF-and TGF-β1-induced EMT in HepG2 cells by deactivating the PI3K/Akt/ERK pathway.

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Section: Methodsmentioning

confidence: 99%

Tanshinone IIA reverses EGF‑ and TGF‑β1‑mediated epithelial‑mesenchymal transition in HepG2 cells via the PI3K/Akt/ERK signaling pathway

Zhang¹,

Lin²,

Chen³

et al. 2019

Oncol Lett

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“…Kaplan-Meier survival analysis for the remaining CCCassociated biomarkers showed a significant correlation between positive expression of ARPC2, GNB1, KCTD10, RPL13A, and TRIO and unfavorable prognosis (OS or OS/DSS), which is in agreement with the RNA-seq results. ARPC2 plays a crucial role in actin polymerization and elevated expression thereof was correlated with unfavorable outcome for breast cancer patients (37). Moreover, a potential drug, benproperine, has been suggested to target ARPC2, and thus inhibit cell migration and metastasis in cell and mouse studies (38).…”

Section: Discussionmentioning

confidence: 99%

Validation of Novel Prognostic Biomarkers for Early-Stage Clear-Cell, Endometrioid and Mucinous Ovarian Carcinomas Using Immunohistochemistry

et al. 2020

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Early-stage (I and II) ovarian carcinoma patients generally have good prognosis. Yet, some patients die earlier than expected. Thus, it is important to stratify early-stage patients into risk groups to identify those in need of more aggressive treatment regimens. The prognostic value of 29 histotype-specific biomarkers identified using RNA sequencing was evaluated for early-stage clear-cell (CCC), endometrioid (EC) and mucinous (MC) ovarian carcinomas (n = 112) using immunohistochemistry on tissue microarrays. Biomarkers with prognostic significance were further evaluated in an external ovarian carcinoma data set using the web-based Kaplan-Meier plotter tool. Here, we provide evidence of aberrant protein expression patterns and prognostic significance of 17 novel histotype-specific prognostic biomarkers [10 for CCC (ARPC2, CCT5, GNB1, KCTD10, NUP155, RPL13A, RPL37, SETD3, SMYD2, TRIO), three for EC (CECR1, KIF26B, PIK3CA), and four for MC (CHEK1, FOXM1, KIF23, PARPBP)], suggesting biological heterogeneity within the histotypes. Combined predictive models comprising the protein expression status of the validated CCC, EC and MC biomarkers together with established clinical markers (age, stage, CA125, ploidy) improved the predictive power in comparison with models containing established clinical markers alone, further strengthening the importance of the biomarkers in ovarian carcinoma. Further, even improved predictive powers were demonstrated when combining these models with our previously identified prognostic biomarkers PITHD1 (CCC) and GPR158 (MC). Moreover, the proteins demonstrated improved risk prediction of CCC-, EC-, and MC-associated ovarian carcinoma survival. The novel histotype-specific prognostic biomarkers may not only improve prognostication and patient stratification of early-stage ovarian carcinomas, but may also guide future clinical therapy decisions.

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“…In addition, TRIM62 is a putative tumor suppressor and the TRIM62 levels represent an important prognostic marker in lung tumor (32) acute myeloid leukemia (AML) (33) and cervical cancer (34). ARPC2 promotes proliferation and metastasis (35,36). REV is an oncogenic retrovirus.…”

Section: Discussionmentioning

confidence: 99%

TRIM62 From Chicken as a Negative Regulator of Reticuloendotheliosis Virus Replication

Niu

Yang

et al. 2020

Front. Vet. Sci.

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Emerging evidence suggests that the tripartite motif containing 62 (TRIM62), a member of the TRIM family, plays an important role in antiviral processes. The objective of the study was to explore the role of TRIM62 in reticuloendotheliosis virus (REV) infection and its potential molecular mechanism. We first demonstrated that the REV infection affected the TRIM62 expression first upregulated and then downregulated in CEF cells. Next, we evaluated the effect of TRIM62 on viral replication. Overexpression of TRIM62 decreased REV replication. On the contrary, silencing of endogenously expressed TRIM62 increased viral replication. Then, to explore the necessity of domains in TRIM62's negative regulation on viral replication, we transfected CEF cells with TRIM62 domain deletion mutants. Deletion domain partially abolished TRIM62's antiviral activity. The effect of SPRY domain deletion was the highest and that of coiled-coil was the lowest. Further, we identified 18 proteins that coimmunoprecipitated and interacted with TRIM62 by immunocoprecipitation and mass spectrometry analysis. Strikingly, among which, both Ras-related protein Rab-5b (RAB5B) and Arp2/3 complex 34-kDa subunit (ARPC2) were involved in actin cytoskeletal pathway. Altogether, these results strongly suggest that chicken TRIM62 provides host defense against viral infection, and all domains are required for its action. RAB5B and ARPC2 may play important roles in its negative regulation processes.Keywords: TRIM62, negative regulation, reticuloendotheliosis virus, domain deletion, RAB5B and ARPC2 FIGURE 2 | Restriction of REV replication in CEF cells induced by TRIM62. (A,B) CEF cells were transfected with pTRIM62 or empty vector before infection with REV. The expression levels of TRIM62 (A) and REV (B) were assessed by qRT-PCR and Western blotting. (C,D) CEF cells were transfected with shTRIM62 or a negative-control shRNA before infection with REV. The expression levels of TRIM62 (C) and REV (D) were determined by qPCR and Western blotting.

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ARPC2 promotes breast cancer proliferation and metastasis

Cited by 27 publications

References 40 publications

Tanshinone IIA reverses EGF‑ and TGF‑β1‑mediated epithelial‑mesenchymal transition in HepG2 cells via the PI3K/Akt/ERK signaling pathway

Tanshinone IIA reverses EGF‑ and TGF‑β1‑mediated epithelial‑mesenchymal transition in HepG2 cells via the PI3K/Akt/ERK signaling pathway

Validation of Novel Prognostic Biomarkers for Early-Stage Clear-Cell, Endometrioid and Mucinous Ovarian Carcinomas Using Immunohistochemistry

TRIM62 From Chicken as a Negative Regulator of Reticuloendotheliosis Virus Replication

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