The comparative attractiveness to female Alfalfa Leafcutting Bees (Vegachile rotundata) of flowers of 209 species of vascular plants, representing 52 families and 154 genera, was examined by measuring visitation rates to bouquets under standardized conditions. The bees were attracted to only 21 species, representing 14 genera and seven families. Two floral features appeared to be related to bee visitation: a flower length less than 13.5 mm; and a corolla tube up to 5 mm in length and up to 3 mm wide. It is speculated that the critical maximum length of 13.5 mm for the flower, and 5 mm length by 3 mm width for the floral tube, are respectively determined by the insect's length, combined head and extended mouthparts, and head width. No relationship was found between attractiveness of the flowers and visible color of the corolla, radial or bilateral symmetry, density of open flowers, and genus and family of plants. Despite its name, the Alfalfa Leafcutting Bee was attracted to Alfalfa (Medicago sativa) half as frequently as it was to White Sweetclover (Melilotus alba) and Purple Loosestrife (Lythrum salicaria).