Array of Metabolites in Italian Hericium erinaceus Mycelium, Primordium, and Sporophore

Abstract

Hericium erinaceus is a medicinal mushroom that contains many molecules promising a plethora of therapeutic properties. In this study, the strain H.e.2 (MicUNIPV, University of Pavia, Italy) was isolated from a sporophore collected in Tuscany (Italy). Mycelium, primordium, and wild type and cultivated sporophores were analyzed by HPLC-UV-ESI/MS. Erinacine A in the mycelium and hericenones C and D in the sporophores were quantified by comparison with their standard molecules. For the first time, H. erinaceus primordium was also investigated for the presence of these molecules. Comparing with the literature data, hericenes, molecules structurally similar to hericenones, were present in all our samples. The highest contents of hericenones C and D were detected in cultivated sporophores, compared to the wild type. The comparison of these data with those of another Italian H. erinaceus strain (H.e.1 MicUNIPV) was discussed. The results led us to select H. erinaceus strains more suitable for mycelium production or sporophore cultivation to obtain extracts with a higher content of bioactive compounds. This work provides a further step towards standardizing the procedures in the development of dietary supplements made from mushrooms.

Neuroprotective Metabolites of Hericium erinaceus Promote Neuro-Healthy Aging

“…Using HPLC-UV-ESI/MS, and by comparison with standard solutions, the presence and amount of different metabolites, i.e., Erinacine A, Hericenone C, Hericenone D was identified and quantified [14,52]. Specifically, He1 extract contained 150 µg/g Erinacine A, 500 µg/g Hericenone C, and about 20 µg/g Hericenone D.…”

“…Based on the lack of formerly published standardized extraction protocol for the ergothioneine (ET), we adopted the method described by Cesaroni et al [97,98] as a starting point, and then rearranged it with the extraction procedure already reported in Corana et al [52] and Ratto et al [14] for erinacines and hericenones, metabolites present in H. erinaceus. In detail, 1 g of lyophilized mycelium or sporophore of He1 was blended with 10 mL of ethanol 70%, and left in the thermostat overnight at 50 C. Before withdrawing, the material was stirred for one hour and was centrifuged at 4000 rpm for three minutes.…”

Optimization of Ultrasonic Extraction to Obtain Erinacine A and Polyphenols with Antioxidant Activity from the Fungal Biomass of Hericium erinaceus

“…The representative HPLC chromatogram of the powder extract of H. erinaceus mycelia is shown in Figure 4 . The retention time at 17.846 represents erinacine A, one of the most characteristic compounds found in general in Hericium species [ 39 ].…”

Notulae to the Italian flora of algae, bryophytes, fungi and lichens: 9

“…This saprotrophic fungus produces white rot on living trees (Boddy et al 2011). In Italy, it is known for Calabria (Siniscalco et al 2018), Toscana (Corana et al 2019), Sardegna, Emilia-Romagna, and Piemonte (Bernicchia and Padovan 1997). Other congener species found in Italy are H. coralloides (Scop.)…”