Arrest of the Cell Cycle Induced by Dibutyryl-Camp and Sodium Butyrate in Root Meristems of Lettuce

Abstract

SUMMARY -Dibutyryl-cAMP was supplied to seedlings of Lactuca sativa L. cv.Great Lakes 118, and its effect on the cell cycle in root meristems was compared to that of dibutyryl-cGMP and sodium butyrate. Dibutyryl-cAMP and sodium butyrate induced a sharp decrease in the labelling index, attributable to an arrest of the cell cycle in G1 and possibly G2, whereas dibutyryl-cGMP was ineffective. The use of dibutyryl-cAMP and sodium butyrate in studies on the control of cell cycle and cell differentiation in growing roots is suggested. Various hypotheses on the mode of action of dibutyryl-cAMP are also discussed.

The effect of butyrate on cell cycle progression in Allium cepa root meristems

“…The results may be summarized as follows: (i) butyrate blocks the meristem cells at specific stages of the cycle; (ii) the blockage is reversible only after limited periods of drug administration; and (iii) this action is apparently not explained by side effects of the drug on protein or nucleic acid synthesis activities. Earlier studies on the action of butyrate on root meristems of Pistim sativum and Lactuca sativa indicated that this drug block cells at G, and G, (Sgorbati et al, 1984, Chiatante et al 1986). Cytophotometrie analysis in asynchronously growing meristem cells suggested that this is also the case in Allium cepa root meristems ( Fig.…”

“…Almost all current knowledge on the action of butyrate has been obtained with animal cell systems. A few aspects, such as the effect of the drug on cell proliferation (Sgorbati et al 1984, Chiatante et al 1986), on hormone-inducible gene expression (Kumar et al 1985) atid on membrane polarity and intracellular pH (Sanders et al 1981, Reid et al, 1985 have been studied in plant cells. Nevertheless, the action of butyrate in plant cell systems is largely unknown.…”

The Supporting Roots of Trees and Woody Plants: Form, Function and Physiology

“…In order to exclude artefacts, we treated pea seedlings with sodium butyrate, which is a compound known to be a strong inhibitor of meristematic cell activity (Chiatante et al, 1986). In this case, the treatments consisted of leaving the pea seedlings in Petri dishes on paper soaked with a medium solution containing sodium butyrate.…”

Short‐chain fatty‐acid‐induced effects on the cell cycle in root meristems of Pisum sativum

“…Butyrate has also been shown to inhibit Are the butyrate-indueed effects specific or are they DNA synthesis in Lactuca sativa meristems using the merely a consequence of acid addition to the medium? pH]-TdR incorporation assay (Chiatante et al 1986). To test the specificity of these effects, several other Studies on the root meristem of Pisum sativum demon-short-chain fatty acids were employed.…”