Arrestin and G Protein Interactions with GPCRs: A Structural Perspective

Barreto, Carlos; Baptista, Salete J.; Bueschbell, Beatriz; Magalhães, Pedro R.; Preto, António; Lemos, Agostinho; Rosário‐Ferreira, Nícia; Schiedel, Anke C.; Machuqueiro, Miguel; Melo, Rita; Moreira, Irina S.

doi:10.1002/9781119564782.ch5

Cited by 3 publications

(1 citation statement)

References 190 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Patients with RA commonly exhibit an altered gut microbiota composition, characterized by decreased microbial diversity and a shift in specific bacterial populations. Notably, there is often a decline in anti-inflammatory bacteria such as Faecalibacterium and an increase in pro-inflammatory genera like Prevotella [13]. This dysbiosis is believed to contribute to the pathogenesis of RA by promoting systemic inflammation and autoimmunity.…”

Section: Introductionmentioning

confidence: 99%

Synergistic impact of Gui Zhi Shao Yao Zhi Mu Decoction and leflunomide on gut microbiota in rheumatoid arthritis: insights from 16S rDNA sequencing

Sun

2024

Am J Transl Res

View full text Add to dashboard Cite

Background: Rheumatoid arthritis (RA) is a chronic autoimmune disease with complex pathogenesis, including alterations in the gut microbiota. Gui Zhi Shao Yao Zhi Mu Decoction (GSZD), a traditional Chinese herbal formula, has shown efficacy in RA treatment, but its impact on intestinal microflora remains unclear. This study aimed to investigate the effects of GSZD combined with leflunomide on the gut microbiota of RA patients. Methods:The study enrolled 48 RA patients who were randomly assigned to either a control group receiving leflunomide or a treatment group receiving GSZD combined with leflunomide for 12 weeks. Gut microbiota composition was analyzed pre-and post-intervention using 16S rDNA sequencing. Changes in microbial diversity, abundance, and metabolic functions were assessed. Results: Post-treatment, both groups exhibited significant alterations in gut microbiota composition. GSZD combined with leflunomide led to an increased Bacteroidetes/Firmicutes ratio and a reduction in Actinobacteria compared to leflunomide alone. This was associated with beneficial shifts in microbial genera and metabolic pathways, suggesting improved gut health and systemic immune modulation. Conclusion: GSZD combined with leflunomide significantly modulates the gut microbiota in RA patients. This study provides insights into the mechanisms underlying the therapeutic effects of GSZD and highlights the potential of integrating traditional Chinese medicine with conventional treatments in managing RA.

show abstract

Section: Introductionmentioning

confidence: 99%

Synergistic impact of Gui Zhi Shao Yao Zhi Mu Decoction and leflunomide on gut microbiota in rheumatoid arthritis: insights from 16S rDNA sequencing

Sun

2024

Am J Transl Res

View full text Add to dashboard Cite

show abstract

Leveraging Artificial Intelligence in GPCR Activation Studies: Computational Prediction Methods as Key Drivers of Knowledge

Caniceiro,

Orzeł,

Rosário-Ferreira

et al. 2024

Methods in Molecular Biology

View full text Add to dashboard Cite

MembIT – A Tool to Calculate Solute Membrane Insertions and Deformations in Molecular Dynamics Simulations

Suzano

Pires

Silva

et al. 2023

J. Comput. Biophys. Chem.

View full text Add to dashboard Cite

In molecular dynamics simulations of membrane systems, it is very important to evaluate the interactions between membrane bilayers and other molecules like small compounds, peptides, or proteins. When these interactions lead to local deformations in the membrane, it is essential to fully understand and accurately characterize them, since these deformations can impact the calculation of membrane properties. Here, we introduce MembIT, a simple standalone tool that calculates several membrane properties that are very useful to gauge system equilibration and quality control in molecular dynamics simulations. It has implemented a distinction between local and bulk lipids, which corrects the local deformations, that are often overlooked by other tools. We show examples of MembIT calculations of membrane insertion and deformation values in systems containing POPC and a small drug (sunitinib), a transmembrane peptide (pHLIP), and a transmembrane protein (ADP/ATP carrier). We hope that this tool will be adopted by the scientific community and that the analyses that it provides become good practices in MD simulations of membrane systems.

show abstract

Arrestin and G Protein Interactions with GPCRs: A Structural Perspective

Cited by 3 publications

References 190 publications

Synergistic impact of Gui Zhi Shao Yao Zhi Mu Decoction and leflunomide on gut microbiota in rheumatoid arthritis: insights from 16S rDNA sequencing

Synergistic impact of Gui Zhi Shao Yao Zhi Mu Decoction and leflunomide on gut microbiota in rheumatoid arthritis: insights from 16S rDNA sequencing

Leveraging Artificial Intelligence in GPCR Activation Studies: Computational Prediction Methods as Key Drivers of Knowledge

MembIT – A Tool to Calculate Solute Membrane Insertions and Deformations in Molecular Dynamics Simulations

Contact Info

Product

Resources

About