Arrhythmogenic right ventricular cardiomyopathy coincided with the cardiac fibrosis in the inner muscle layer of the left ventricular wall in a boxer dog

Yamada, Naoaki; Kitamori, Takashi; Kitamori, Fumiyo; Ishigami, Kanako; Iwanaga, Koji; Itou, T; Kobayashi, Ryosuke; Kumabe, Shino; Doi, Takuya; Sato, Junko; Wako, Yumi; Tsuchitani, Minoru

doi:10.1292/jvms.14-0513

Cited by 3 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A análise histopatológica de uma Boxer com CADV demonstrou espessamento da túnica íntima da camada muscular interna de pequenas artérias que pode ter induzido a isquemia regional com lesão a células cardíacas (Yamada et al, 2015).…”

Section: Patologiaunclassified

Reação Em Cadeia Da Polimerase (Pcr) Para O Diagnóstico De Coinfecção Por Ehrlichia Canis E Babesia Sp. Em Cães Com Hepatozoonose

Cavalcanti,

Abreu,

Guedes

et al. 2022

Tópicos Em Sanidade De Cães E Gatos, Volume 2

View full text Add to dashboard Cite

Section: Patologiaunclassified

Reação Em Cadeia Da Polimerase (Pcr) Para O Diagnóstico De Coinfecção Por Ehrlichia Canis E Babesia Sp. Em Cães Com Hepatozoonose

Cavalcanti,

Abreu,

Guedes

et al. 2022

Tópicos Em Sanidade De Cães E Gatos, Volume 2

View full text Add to dashboard Cite

“…Two distinct histopathological variations of canine DCM have been described; attenuated wavy fiber type and fibro‐fatty infiltration type . Fibro‐fatty infiltration is considered analogous to arrhythmogenic right ventricular cardiomyopathy (ARVC) in humans because of the comparable pathological changes and clinical presentation between the two species . Although the pathophysiologic mechanism underlying ARVC remains unclear, it is thought to include molecular pathways involved in the formation of mechanical and electrical coupling, apoptosis, and migration and differentiation of epicardial‐derived cells .…”

mentioning

confidence: 99%

Cryopreservation of canine cardiosphere‐derived cells: Implications for clinical application

et al. 2017

View full text Add to dashboard Cite

The clinical application of cardiosphere-derived cells (CDCs) to treat cardiac disease has gained increasing interest over the past decade. Recent clinical trials confirm their regenerative capabilities, although much remains to be elucidated about their basic biology. To develop this new treatment modality, in a cost effective and standardized workflow, necessitates the creation of cryopreserved cell lines to facilitate access for cardiac patients requiring urgent therapy. Cryopreservation may however lead to alterations in cell behavior and potency. The aim of this study was to investigate the effect of cryopreservation on canine CDCs. CDCs and mesenchymal stem cells (MSCs) isolated from five dogs were characterized. CDCs demonstrated a population doubling time that was unchanged by cryopreservation (fresh vs. cryopreserved; 57.13 6 5.27 h vs. 48.94 6 9.55 h, P 5 0.71). This was slower than for MSCs (30.46 h, P < 0.05). The ability to form clones, self-renew, and commit to multiple lineages was unaffected by cryopreservation. Cryopreserved CDCs formed larger cardiospheres compared to fresh cells (P < 0.0001). Fresh CDCs showed a high proportion of CD105 1 (89.0% 6 4.98) and CD44 1 (99.68% 6 0.13) cells with varying proportions of CD90 1 (23.36% 6 9.78), CD34 1 (7.18% 6 4.03) and c-Kit 1 (13.17% 6 8.67) cells. CD45 1 (0.015% 6 0.005) and CD29 1 (2.92% 6 2.46) populations were negligible. Increasing passage number of fresh CDCs correlated with an increase in the proportion of CD34 1 and a decrease in CD90 1 cells (P 5 0.003 and 0.03, respectively). Cryopreserved CDCs displayed increased CD34 1 (P < 0.001) and decreased CD90 1 cells (P 5 0.042) when compared to fresh cells. Overall, our study shows that cryopreservation of canine CDCs is feasible without altering their stem characteristics, thereby facilitating their utilization for clinical trials. V C 2017 International Society for Advancement of Cytometry

show abstract

Animal models and animal-free innovations for cardiovascular research: current status and routes to be explored. Consensus document of the ESC Working Group on Myocardial Function and the ESC Working Group on Cellular Biology of the Heart

Velden

Asselbergs

Bakkers

et al. 2022

Cardiovascular Research

View full text Add to dashboard Cite

Cardiovascular diseases represent a major cause of morbidity and mortality, necessitating research to improve diagnostics, and to discover and test novel preventive and curative therapies. All of which warrant experimental models that recapitulate human disease. The translation of basic science results to clinical practice is a challenging task. In particular for complex conditions such as cardiovascular diseases, which often result from multiple risk factors and co-morbidities. This difficulty might lead some individuals to question the value of animal research, citing the translational ‘valley of death’, which largely reflects the fact that studies in rodents are difficult to translate to humans. This is also influenced by the fact that new, human-derived in vitro models can recapitulate aspects of disease processes. However, it would be a mistake to think that animal models cannot provide a vital step in the translational pathway as they do provide important pathophysiological insights into disease mechanisms particularly on a organ and systemic level. While stem cell-derived human models have the potential to become key in testing toxicity and effectiveness of new drugs, we need to be realistic, and carefully validate all new human-like disease models. In this position paper, we highlight recent advances in trying to reduce the number of animals for cardiovascular research ranging from stem cell-derived models to in situ modelling of heart properties, bioinformatic models based on large datasets, and improved current animal models, which show clinically relevant characteristics observed in patients with a cardiovascular disease. We aim to provide a guide to help researchers in their experimental design to translate bench findings to clinical routine taking the replacement, reduction and refinement (3R) as a guiding concept.

show abstract

Arrhythmogenic right ventricular cardiomyopathy coincided with the cardiac fibrosis in the inner muscle layer of the left ventricular wall in a boxer dog

Cited by 3 publications

References 13 publications

Reação Em Cadeia Da Polimerase (Pcr) Para O Diagnóstico De Coinfecção Por Ehrlichia Canis E Babesia Sp. Em Cães Com Hepatozoonose

Reação Em Cadeia Da Polimerase (Pcr) Para O Diagnóstico De Coinfecção Por Ehrlichia Canis E Babesia Sp. Em Cães Com Hepatozoonose

Cryopreservation of canine cardiosphere‐derived cells: Implications for clinical application

Animal models and animal-free innovations for cardiovascular research: current status and routes to be explored. Consensus document of the ESC Working Group on Myocardial Function and the ESC Working Group on Cellular Biology of the Heart

Contact Info

Product

Resources

About