Arsenic Binding to Proteins

Shen, Shengwen; Li, Xing‐Fang; Cullen, William R.; Weinfeld, Michael; Le, X. Chris

doi:10.1021/cr300015c

Cited by 706 publications

(609 citation statements)

References 404 publications

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“…10,11 Developing cost-effective biosensors might provide a complementary solution. 12 For example, genetically engineered bacterial cells, 13 and enzyme inhibition assays were developed to detect arsenic. 14,15 On the chemical sensor side, a few optical and electrochemistry assays were reported.…”

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confidence: 99%

DNA adsorption by magnetic iron oxide nanoparticles and its application for arsenate detection

2014

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DNA adsorption by magnetic iron oxide nanoparticles and its application for arsenate detection

2014

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“…The results of this work and recent epidemiologic knowledge (16,17,62)allow concluding that the arsenic accumulation and their speciation in cardiovascular tissues, particularly in human beings subjected to chronic arsenic exposure, they are factors of risk fortheir heart health. In particular, the auricle (AU)behaves as an ‗‗As +3 '' target tissue, which is one of the most toxic arsenic species.…”

Section: Discussionmentioning

confidence: 70%

Environmental Contamination of Arsenic and its Toxicological Impact in Cardiovascular Tissues of Patients with Coronary Heart Disease

2017

IJSR

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Background: The concentration levels of As in the Chilean II Region of Antofagasta produces non cancer health outcomes such as cardiovascular diseases and in last term heart attack. On this study, the determination of total As content and main inorganic and organoarsenic species found in three heart tissues (auricle, mammary artery and fat) and the saphenous vein of people living in the Chilean II Region, suffering coronary thrombosis has been carried out. Comparison with similar tissues of patients from other noncontaminated areas has been undertaken. Methods: Total As determination has been performed by HG-AAS and ICP-MS. For As species have been used the tandem HPLC-ICP-MS using the Hamilton PRP X100 anion column and methanol:water (1:1) extraction. Results: The auricle and in less extend the saphenous vein support the higher As concentration (mean values of 7.7 and 2.5 µg g -1 , respectively), being As(III) the predominant species. Methylation towards MMA and DMA is not a favoured mechanism. Conclusions: The presence of high total As and high As(III) species content in the auricle and saphenous vein of more contaminated people, the damage found in the saphenous vein tissue and the global characteristics of the people under study in which the As stigmas are present in all of them, suggests that As could be involved in the cardiovascular diseases.

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“…K m is important in enzyme kinetic because its value includes not only the affinity of substrate for the enzyme but also the rate at which the enzyme-bound substrate is converted to the product in the catalytic reaction. Thus, K m value can be interpreted as a crude measurement of the affinity of the substrate for the enzyme [55].…”

Section: Kinetics Analysismentioning

confidence: 99%

Characterization of β-galactosidase in the Crude Plant Extract of <i>Artemisia judaica</i> L. in Presence and Absence of Some Heavy Metals

Atrooz¹,

Abukhalil²,

Al-Rawashdeh³

2016

AJLS

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β-galactosidase (EC 3.2.1.23) is important in the formation of a medicinal plant Artemisia judaica (al-ba'atharan) aroma. The crude plant extracts of Artemisia judaica were used to characterize the enzyme in the term of pH, temperature, enzyme kinetic and effects of some heavy metals on its activity. The enzyme activity was measured by its ability to hydrolyze the substrate 2-nitrophenyl β-D-galactopyranoside (ONPG). The enzyme activity was reached maximum at 50°C and at pH 6.0. The K m and V max values of the enzyme were 3.6 mM and 1.67 µmol/min, respectively. Uncompetitive inhibition was observed in presence of Hg +2 , Fe +3 and Zn +2 for the enzyme β-galactosidase in the crude extract through the decrease in the K m and V max values. Pb +2 and Cu +2 were found to act as a noncompetitive inhibitors on the enzyme β-galactosidase in the crude extract due to increase in the K m values and decrease in V max values. The study showed that Hg +2 was the most potent inhibitor while Cu +2 exhibited the least inhibition degree on β-galactosidase activity in the Artemisia judaica. These finding indicated that the enzyme β-galactosidase in the crude leaves extract of Artemisia judaica can be used in industrial and medical applications.

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Arsenic Binding to Proteins

Cited by 706 publications

References 404 publications

DNA adsorption by magnetic iron oxide nanoparticles and its application for arsenate detection

DNA adsorption by magnetic iron oxide nanoparticles and its application for arsenate detection

Environmental Contamination of Arsenic and its Toxicological Impact in Cardiovascular Tissues of Patients with Coronary Heart Disease

Characterization of β-galactosidase in the Crude Plant Extract of <i>Artemisia judaica</i> L. in Presence and Absence of Some Heavy Metals

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Arsenic Binding to Proteins

Cited by 706 publications

References 404 publications

DNA adsorption by magnetic iron oxide nanoparticles and its application for arsenate detection

DNA adsorption by magnetic iron oxide nanoparticles and its application for arsenate detection

Environmental Contamination of Arsenic and its Toxicological Impact in Cardiovascular Tissues of Patients with Coronary Heart Disease

Characterization of β-galactosidase in the Crude Plant Extract of &lt;i&gt;Artemisia judaica&lt;/i&gt; L. in Presence and Absence of Some Heavy Metals

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Characterization of β-galactosidase in the Crude Plant Extract of <i>Artemisia judaica</i> L. in Presence and Absence of Some Heavy Metals