Arsenic detoxification potential of aox genes in arsenite-oxidizing bacteria isolated from natural and constructed wetlands in the Republic of Korea

Chang, Jin-Soo; Yoon, In-Ho; Lee, Jihoon; Kim, Ki-Rak; An, Jeong-Yi; Kim, Kyoung‐Woong

doi:10.1007/s10653-009-9268-z

Cited by 65 publications

(31 citation statements)

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“…The aerobic arsenite oxidases involved in such processes are heterodimers consisting of a large subunit with a molybdenum center and a [3Fe-4S] cluster (AroA, AsoA, and AoxB) and a small subunit containing a Rieske-type [2Fe-2S] cluster (AroB, AsoB, and AoxA) (1, 13). The large subunit in these enzymes is similar to that found in other members of the dimethyl sulfoxide (DMSO) reductase family of molybdenum enzymes but is clearly phylogenetically divergent from the respiratory arsenate reductases (ArrA) or other proteins of the DMSO reductase family of molybdenum oxidoreductases, such as the new arsenite reductase described recently for Alkalilimnicola ehrlichii (25,31,40).aox genes have been identified in 25 bacterial and archaeal genera isolated from various arsenic-rich environments, most of which belong to the Alpha-, Beta-, or Gammaproteobacteria phylum (7,10,12,14,23,25,29,32,37). Recent studies based on environmental DNA extracted from soils, sediments, and geothermal mats with different chemical characteristics and various levels of arsenic contamination have suggested that the distribution and the diversity of arsenite-oxidizing microorganisms may be greater than previously suggested (6, 10, 14-16, 18, 28, 29).…”

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“…aox genes have been identified in 25 bacterial and archaeal genera isolated from various arsenic-rich environments, most of which belong to the Alpha-, Beta-, or Gammaproteobacteria phylum (7,10,12,14,23,25,29,32,37). Recent studies based on environmental DNA extracted from soils, sediments, and geothermal mats with different chemical characteristics and various levels of arsenic contamination have suggested that the distribution and the diversity of arsenite-oxidizing microorganisms may be greater than previously suggested (6, 10, 14-16, 18, 28, 29).…”

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Unsuspected Diversity of Arsenite-Oxidizing Bacteria as Revealed by Widespread Distribution of theaoxBGene in Prokaryotes

Heinrich-Salmeron

Cordi

Brochier-Armanet

et al. 2011

Appl Environ Microbiol

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In this study, new strains were isolated from an environment with elevated arsenic levels, Sainte-Marieaux-Mines (France), and the diversity of aoxB genes encoding the arsenite oxidase large subunit was investigated. The distribution of bacterial aoxB genes is wider than what was previously thought. AoxB subfamilies characterized by specific signatures were identified. An exhaustive analysis of AoxB sequences from this study and from public databases shows that horizontal gene transfer has likely played a role in the spreading of aoxB in prokaryotic communities.Arsenic, which is one of the most toxic metalloids, is distributed ubiquitously but not uniformly around the world. Levels of arsenic differ considerably from one geographical region to another, depending on the geochemical characteristics of the soil (natural contamination) and the industrial activities carried out in the vicinity (anthropogenic contamination) (22). In aquatic environments, arsenic occurs mainly in the form of the inorganic species arsenate [As(V)] and arsenite [As(III)]; the latter species, which is more bioavailable, is usually thought to have more-toxic effects on prokaryotes than As(V) (34). As(III) oxidation leads to the formation of the less available form As(V), which can either precipitate with iron [Fe(III)] or be adsorbed by ferrihydrite. The oxidation process may be mediated by microbial activities, which contribute to the natural remediation processes observed in contaminated environments (21,26,27,34). Consequently, bioprocesses for the treatment of arsenic-contaminated waters have been developed based on the precipitation or adsorption of the As(V) produced by bacteria (4, 9, 21). Some well-known prokaryotes oxidize As(III) into As(V) under aerobic (e.g., Herminiimonas arsenicoxydans, Thiomonas spp., or Rhizobium sp. strain NT26) or anaerobic (e.g., Alkalilimnicola ehrlichii) conditions as part of a detoxification process (12,17,31,32,39). Some chemolithotrophs also use arsenite as an electron donor (e.g., Rhizobium sp. strain NT26 or Thiomonas arsenivorans) (5, 32). The aerobic arsenite oxidases involved in such processes are heterodimers consisting of a large subunit with a molybdenum center and a [3Fe-4S] cluster (AroA, AsoA, and AoxB) and a small subunit containing a Rieske-type [2Fe-2S] cluster (AroB, AsoB, and AoxA) (1, 13). The large subunit in these enzymes is similar to that found in other members of the dimethyl sulfoxide (DMSO) reductase family of molybdenum enzymes but is clearly phylogenetically divergent from the respiratory arsenate reductases (ArrA) or other proteins of the DMSO reductase family of molybdenum oxidoreductases, such as the new arsenite reductase described recently for Alkalilimnicola ehrlichii (25,31,40).aox genes have been identified in 25 bacterial and archaeal genera isolated from various arsenic-rich environments, most of which belong to the Alpha-, Beta-, or Gammaproteobacteria phylum (7,10,12,14,23,25,29,32,37). Recent studies based on environmental DNA extracted from soils, sediments, a...

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confidence: 99%

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confidence: 99%

Unsuspected Diversity of Arsenite-Oxidizing Bacteria as Revealed by Widespread Distribution of theaoxBGene in Prokaryotes

Heinrich-Salmeron

Cordi

Brochier-Armanet

et al. 2011

Appl Environ Microbiol

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“…Both As(III) oxidizers and As(V) reducers in Pseudomonas have been isolated before. P. stutzeri GIST-BDan2 (EF429003) is an As(III)-oxidizing strain isolated from constructed wetlands (Chang et al, 2010). Three arsenate reducers of Pseudomonas were isolated in As-rich groundwater (Liao et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Arsenic redox transformation by Pseudomonas sp. HN-2 isolated from arsenic-contaminated soil in Hunan, China

Zhang

Yin

Cai

et al. 2016

Journal of Environmental Sciences

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A mesophilic, Gram-negative, arsenite[As(III)]-oxidizing and arsenate[As(V)]-reducing bacterial strain, Pseudomonas sp. HN-2, was isolated from an As-contaminated soil. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the strain was closely related to Pseudomonas stutzeri. Under aerobic conditions, this strain oxidized 92.0% (61.4 μmol/L) of arsenite to arsenate within 3 hr of incubation. Reduction of As(V) to As(III) occurred in anoxic conditions. Pseudomonas sp. HN-2 is among the first soil bacteria shown to be capable of both aerobic As(III) oxidation and anoxic As(V) reduction. The strain, as an efficient As(III) oxidizer and As(V) reducer in Pseudomonas, has the potential to impact arsenic mobility in both anoxic and aerobic environments, and has potential application in As remediation processes.

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“…Many bacteria are also able to reduce arsenate as a means of detoxification using ArsC, an arsenate reductase that is capable of converting intracellular arsenate into arsenite, which is further transported out of the cell by an energy-dependent efflux process (14-16). Arsenite-oxidizing bacteria (AOB) can oxidize As(III) into As(V) under aerobic or anaerobic conditions (17)(18)(19)(20)(21)(22)(23)(24)(25). Under aerobic conditions, AOB are able to convert As(III) into As(V) by using As(III) as an electron donor and oxygen as an electron acceptor (19-23); under anaerobic conditions, some AOB can convert As(III) into As(V) by using As(III) as an electron donor and nitrate, selenate, or chlorate as an electron acceptor (18,(24)(25)(26)(27).…”

Section: Importancementioning

confidence: 99%

“…Arsenite-oxidizing bacteria (AOB) can oxidize As(III) into As(V) under aerobic or anaerobic conditions (17)(18)(19)(20)(21)(22)(23)(24)(25). Under aerobic conditions, AOB are able to convert As(III) into As(V) by using As(III) as an electron donor and oxygen as an electron acceptor (19)(20)(21)(22)(23); under anaerobic conditions, some AOB can convert As(III) into As(V) by using As(III) as an electron donor and nitrate, selenate, or chlorate as an electron acceptor (18,(24)(25)(26)(27). AOB are either chemoautotrophic, using carbon dioxide as the sole carbon source, or heterotrophic, using organic carbon as the sole carbon source.…”

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Functions and Unique Diversity of Genes and Microorganisms Involved in Arsenite Oxidation from the Tailings of a Realgar Mine

Zeng

Guoji

Wang

et al. 2016

Appl Environ Microbiol

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The tailings of the Shimen realgar mine have unique geochemical features. Arsenite oxidation is one of the major biogeochemical processes that occurs in the tailings. However, little is known about the functional and molecular aspects of the microbial community involved in arsenite oxidation. Here, we fully explored the functional and molecular features of the microbial communities from the tailings of the Shimen realgar mine. We collected six samples of tailings from sites A, B, C, D, E, and F. Microcosm assays indicated that all of the six sites contain both chemoautotrophic and heterotrophic arsenite-oxidizing microorganisms; their activities differed considerably from each other. The microbial arsenite-oxidizing activities show a positive correlation with soluble arsenic concentrations. The microbial communities of the six sites contain 40 phyla of bacteria and 2 phyla of archaea that show extremely high diversity. Soluble arsenic, sulfate, pH, and total organic carbon (TOC) are the key environmental factors that shape the microbial communities. We further identified 114 unique arsenite oxidase genes from the samples; all of them code for new or new-type arsenite oxidases. We also isolated 10 novel arsenite oxidizers from the samples, of which 4 are chemoautotrophic and 6 are heterotrophic. These data highlight the unique diversities of the arsenite-oxidizing microorganisms and their oxidase genes from the tailings of the Shimen realgar mine. To the best of our knowledge, this is the first report describing the functional and molecular features of microbial communities from the tailings of a realgar mine. IMPORTANCEThis study focused on the functional and molecular characterizations of microbial communities from the tailings of the Shimen realgar mine. We fully explored, for the first time, the arsenite-oxidizing activities and the functional gene diversities of microorganisms from the tailings, as well as the correlation of the microbial activities/diversities with environmental factors. The findings of this study help us to better understand the diversities of the arsenite-oxidizing bacteria and the geochemical cycle of arsenic in the tailings of the Shimen realgar mine and gain insights into the microbial mechanisms by which the secondary minerals of the tailings were formed. This work also offers a set of unique arsenite-oxidizing bacteria for basic research of the molecular regulation of arsenite oxidation in bacterial cells and for the environmentally friendly bioremediation of arsenic-contaminated groundwater.A rsenic is distributed ubiquitously in the earth's crust and is toxic to life in some forms. Levels of arsenic differ considerably from one geographical region to another, depending on the geochemical conditions of the sites and the anthropogenic activities carried out in the vicinity (1, 2). Arsenic occurs naturally in organic and inorganic forms. Arsenic typically exists in one of the following four oxidation states: As(V), As(III), As(ϪIII), and As(0). The dominant forms are As(III) (...

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Arsenic detoxification potential of aox genes in arsenite-oxidizing bacteria isolated from natural and constructed wetlands in the Republic of Korea

Cited by 65 publications

References 43 publications

Unsuspected Diversity of Arsenite-Oxidizing Bacteria as Revealed by Widespread Distribution of theaoxBGene in Prokaryotes

Unsuspected Diversity of Arsenite-Oxidizing Bacteria as Revealed by Widespread Distribution of theaoxBGene in Prokaryotes

Arsenic redox transformation by Pseudomonas sp. HN-2 isolated from arsenic-contaminated soil in Hunan, China

Functions and Unique Diversity of Genes and Microorganisms Involved in Arsenite Oxidation from the Tailings of a Realgar Mine

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