Arsenic-induced genotoxic responses and their amelioration by diphenylene iodonium, 24-epibrassinolide and proline in Glycine max L.

Chandrakar, Vibhuti; Yadu, Bhumika; Meena, Rakesh Kumar; Dubey, Amit; Keshavkant, S.

doi:10.1016/j.plaphy.2016.12.023

Cited by 69 publications

(31 citation statements)

References 30 publications

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“…Excessive ROS induce overproduction of MDA, a marker of lipid peroxidation reaction in stressed cells . The results recorded in the present study (Table ) are consistent with the previous report under As stress . Additionally, As‐induced enhanced LOX was also shown to be responsible for higher lipolytic activity and oxidation of lipid moieties in membranes.…”

Section: Discussionsupporting

confidence: 92%

“…Therefore, in the present study, the authors have examined the improvement in As stress tolerance capacity of G. max L. after exogenous application of a NO donor (SNP) and DMTU, separately, by evaluating a number of physiological, biochemical and molecular indices related to As adaptation. Under As stress, germination percentage, radicle length and biomass (FM and DM) accrual were significantly hampered as was previously reported . The decrease in these growth parameters might be due to the disruption in cell division and elongation, and energy flow by As .…”

Section: Discussionsupporting

confidence: 65%

“…This study determined the enhanced accumulation of As in the radicles of G. max L. subjected to As solution, which may be linked with the alterations in the cell membrane and cell wall . On the other hand, exogenous application of NO or DMTU significantly declined the As accrual and was more prominent with NO (Figure ).…”

Section: Discussionmentioning

confidence: 92%

“…Its two inorganic forms; arsenate and arsenite exist in nature, later being more toxic than the former. Exposure of plants to As results enhanced production of reactive oxygen species (ROS) such as superoxide (

O_{2}^{-}

), hydrogen peroxide (H 2 O 2 ), hydroxyl radical, etc., that not only diminishes membrane stability but also oxidizes cellular macromolecules like lipid, protein, and DNA . Lipid moieties, particularly polyunsaturated fatty acid fractions, of the cell are also oxidized by lipoxygenase (LOX) enzyme .…”

Section: Introductionmentioning

confidence: 99%

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Nitric oxide and dimethylthiourea up‐regulates pyrroline‐5‐carboxylate synthetase expression to improve arsenic tolerance in Glycine max L.

Chandrakar

Keshavkant

2018

Env Prog and Sustain Energy

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Arsenic (As) is a toxic metalloid, severely affecting the normal functioning of plants, animals, and human beings. Nitric oxide (NO) and dimethylthiourea (DMTU) are two potential compounds play key roles in enhancing tolerance against a variety of abiotic stresses in plants. In the present study, ameliorating abilities of both NO and DMTU against As‐induced disturbances in Glycine max L. have been investigated. Overall results revealed that As accrual significantly suppressed seed germination, radicle length, and biomass accumulation. The oxidative stress markers such as membrane integrity, contents of reactive oxygen species, and malondialdehyde, and lipoxygenase activity were enhanced by As treatment. Pronounced decreases in protein and DNA contents with a concomitant increase in DNA oxidation were observed in the growing radicles after exposure to As. In addition, treatment of As also revealed elevation in the activity and gene expression of pyrroline‐5‐carboxylate synthetase, in parallel to both proline and sugar accumulation. However, the toxic effects of As were partially/ to a large extent reversed by the exogenous application of NO and DMTU, separately, in Glycine max L. Moreover, between these two treatments, NO was more efficient than the DMTU against As‐induced oxidative stress responses in Glycine max L. Our findings provide a foundation to minimize the toxic effects of As in crop plants worldwide. © 2018 American Institute of Chemical Engineers Environ Prog, 38: 402–409, 2019

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 92%

O_{2}^{-}

Section: Introductionmentioning

confidence: 99%

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Nitric oxide and dimethylthiourea up‐regulates pyrroline‐5‐carboxylate synthetase expression to improve arsenic tolerance in Glycine max L.

Chandrakar

Keshavkant

2018

Env Prog and Sustain Energy

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“…This over-produced ROS are largely shown to attack cellular macromolecules such as lipids, proteins, nucleic acids, etc. (Chandrakar et al 2017a).…”

Section: Introductionmentioning

confidence: 99%

Modulation of arsenic-induced oxidative stress and protein metabolism by diphenyleneiodonium, 24-epibrassinolide and proline in Glycine max L.

Chandrakar¹,

Dubey²,

Keshavkant³

2018

Acta Botanica Croatica

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Arsenic (As)-toxicity is a major constraint for crop production. The present study was intended to examine the comparative ameliorative effects of diphenyleneiodonium (DPI), 24-epibrassinolide (EBL) and proline (Pro) on As-stress inGlycine maxL. Seeds ofGlycine maxL. were subjected to As (100 µM) singly, and together with DPI (10 µM), EBL (0.5 µM) or Pro (10 mM), for five days, and were then analyzed. Experimental results showed that As treatment caused a substantial fall in growth traits like germination percentage, radicle length and dry mass, which was accompanied by As accumulation. Additionally, As application also revealed reduced viability, total protein content and activities of antioxidative enzymes (superoxide dismutase, catalase and ascorbate peroxidase), while it increased the levels of total sugar, proline and oxidative stress markers such as electrolyte leakage, reactive oxygen species, lipid oxidized products, protein carbonyls and hydroperoxides, Amadori and Maillard reaction products, malondialdehyde-/4-hydroxy-2-nonenal-protein adducts, protease and proteasome. Isozymes of antioxidative enzymes were also observed to be altered considerably under As-stress. Impressively, DPI, EBL and Pro played their role as protective agents, hence caused enhanced growth and reduced As accumulation. These protective chemicals also improved the viability, accruals of total protein, total sugar and endogenous proline, and activities of antioxidants, while they reduced the levels of oxidative stress markers. Our findings demonstrated the involvement of DPI, EBL and Pro in As-stress tolerance inGlycine maxL. Further, Pro appears to be superior to DPI and EBL, in alleviating As-induced responses inGlycine maxL.

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Molecular Investigation of Metalloid Stress Response in Higher Plants

Landi

Paradisone

Esposito

2020

Metalloids in Plants

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Arsenic-induced genotoxic responses and their amelioration by diphenylene iodonium, 24-epibrassinolide and proline in Glycine max L.

Cited by 69 publications

References 30 publications

Nitric oxide and dimethylthiourea up‐regulates pyrroline‐5‐carboxylate synthetase expression to improve arsenic tolerance in Glycine max L.

Nitric oxide and dimethylthiourea up‐regulates pyrroline‐5‐carboxylate synthetase expression to improve arsenic tolerance in Glycine max L.

Modulation of arsenic-induced oxidative stress and protein metabolism by diphenyleneiodonium, 24-epibrassinolide and proline in Glycine max L.

Molecular Investigation of Metalloid Stress Response in Higher Plants

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