Arsenic induced hematological and biochemical responses in nutritionally important catfish Clarias batrachus (L.)

Abstract

The impact of sublethal toxicity of sodium arsenite on hematological and certain biochemical parameters of the fresh water catfish Clarias batrachus has been analyzed following exposure of sublethal concentration (1 mg/L; 5% of LC50 value) of sodium arsenite for 10, 30, 45, and 60 days. Arsenic bioaccumulation in the blood tissue of the fish increased progressively with increased period of exposure. The values of total erythrocyte count (TECs), total leucocytes count (TLCs), hemoglobin concentration, and packed cell volume (PCV) 1.40 ± 0.03 × 106/mm3, 174.83 ± 2.74 × 103/mm3, 5.01 ± 0.26 g/100 ml, 25.00 ± 1.06 were observed respectively at the end of 60 days of exposure. The results of hematological indices were found to be 179.23 ± 8.81fl/cell for mean corpuscular volume (MCV), 35.92 ± 1.89 pg/cell for mean corpuscular hemoglobin (MCH) and 20.17 ± 1.12 g/dl for mean corpuscular hemoglobin concentration (MCHC). The present findings are clearly indicating severe fish anemia due to the arsenic salt exposure. The continued arsenic toxicity results in decreased serum protein concentration that might be a cause for the loss of weight as well as weakness in the fish.

Significance Assessment of Amphora coffeaeformis in Arsenic-Induced Hemato- Biochemical Alterations of African Catfish (Clarias gariepinus)

“…Arsenic-induced changes in MCV, MCH, and WBCs of current species were evident. Some other studies reported similar results with fluctuations in some fish such as Clarias batrachus, and Catla catla (Lavanya et al, 2011;Kumar and Banerjee, 2016).…”

“…The liver is the major organ involved in the regulation of metabolic functions and most of the biotransformation of inorganic arsenic takes place in the liver (Kumar and Banerjee, 2016;Kumari et al, 2017). So, analysis of serum AST and ALT were widely used to demonstrate arsenic induced-hepatotoxicity (Dorcas and Solomon, 2014).…”

Biochemical alterations due to carbaryl exposure in glucose content of liver and alimentary canal of Clarias batrachus

“…These changes possibly deteriorate the secretion of enzyme in the gut and absorption is also decreased due to damaged vili of intestine. This is also supported by the fi nding of Adewoyeso (2010); Kumar and Banerjee (2016) as they have observed depletion in glucose content in the fi sh Clarias garipinus.…”

Change of growth performance, hematological parameters, and plasma component by hexavalent chromium exposure in starry flounder, Platichthys stellatus

“…Values are mean ± SE. As determined by Duncan's multiple range test, the other values of superscript were significantly different at 2 and 4 weeks (P < 0.05) by decreasing the oxygen supply due to red blood cell concentration and decreased hemoglobin (Kumar and Banerjee 2016). Hepatosomatic index is used as an important indicator of health status in aquatic animals manifesting the toxic effects of metal exposure (Datta et al 2007;Bolger and Connolly 1989).…”

“…Plasma glucose is a reliable indicator of multiple stress factors in fish, including heavy metals, and is commonly increased by carbohydrate metabolism (CiCiK and ENGiN 2005). The increase in glucose level is attributed to the breakdown of proteins and high-density lipids, resulting in the release of carbohydrates, and decreased lipid and protein levels, following metal toxicity (Kumar and Banerjee 2016). Plasma cortisol levels are widely used in the primary response to stressors such as metals and insecticides; cortisol and other corticosteroid hormones maintain homeostasis under toxicity (Fırat et al 2011).…”