Arsenic speciation transformation and arsenite influx and efflux across the cell membrane of fungi investigated using HPLC–HG–AFS and in-situ XANES

Zeng, Xibai; Su, Shiming; Feng, Qiufen; Wang, Xiurong; Yang-zhu, Zhang; Zhang, Lili; Jiang, Sheng; Li, Aiguo; Li, Lianfang; Wang, Yanan; Wu, Cuixia; Bai, Lei; Duan, Renyan

doi:10.1016/j.chemosphere.2014.10.034

Cited by 16 publications

(7 citation statements)

References 26 publications

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“…Similarly, the uptake of As(V) in many organisms (e.g., algae, fungi, plants and amphibians) has been reported to be mediated by P due to their structural analogy. 20,[47][48][49][50] Our results also revealed that As(V) treatment reduced the PO 4 3À assimilation rate (Fig. 3 and Tables 1 and S3 †) from the culture, and provided further evidence for the competition between As(V) and PO 4 3À to be taken up by algal cells.…”

Section: Discussionsupporting

confidence: 57%

Arsenate toxicity and metabolism in the halotolerant microalga Dunaliella salina under various phosphate regimes

Wang

Zheng

Liu

et al. 2016

Environ. Sci.: Processes Impacts

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Microalgae play an important role in arsenic (As) biogeochemical cycles as they are capable of accumulating and metabolizing this metalloid efficiently. This study aimed to investigate the toxicity, accumulation and transformation of arsenate (As(v)) in Dunaliella salina, an exceptionally halotolerant microalga, under various phosphate (PO4(3-)) regimes. The results of the 72-h toxicity test showed that D. salina was tolerant to As(v). In addition, the toxicity of As(v) was mitigated by an increased PO4(3-) supply. D. salina resisted the adverse effects of As(v) through the suppression of As uptake, enhancement of As reduction, methylation in the cell and excretion from the cell. Our study revealed that D. salina reduced As(v) toxicity using different strategies, i.e., reduction of As uptake upon acute As stress (24 h) and increase of As efflux following chronic As exposure (9 day). Moreover, PO4(3-) strongly affected the adsorption, uptake and transformation of As(v) in D. salina. As(v) reduction, DMA production and As excretion were enhanced under P-limited conditions (0.112 mg L(-1)) or upon higher As(v) exposure (1120 μg L(-1)). Furthermore, PO4(3-) had a significant influence on the As removal ability of D. salina. A high As removal efficiency (>95.6%) was observed in the 5-day cultures at an initial As concentration of 11.2 μg L(-1) and PO4(3-) of 0.112 and 1.12 mg L(-1). However, only 10.9% of total As was removed under 11.2 mg L(-1) PO4(3-) after 9 days of incubation. The findings of this study illustrate the pivotal roles of extracellular PO4(3-) in As(v) toxicity and metabolism, and the results may be relevant for future research on the minimization of As contamination in algal products as well as on the enhancement of As removal from the environment.

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Section: Discussionsupporting

confidence: 57%

Arsenate toxicity and metabolism in the halotolerant microalga Dunaliella salina under various phosphate regimes

Wang

Zheng

Liu

et al. 2016

Environ. Sci.: Processes Impacts

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“…Yeast was found to be able to methylate inorganic As into organic As in soil environment. 7 Zeng et al 36 found that As (III) might be oxidated and methylated by some fungi (e.g. Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1) and generate As(V), MMA, and DMA.…”

Section: Resultsmentioning

confidence: 99%

Concentrations of Total As and As Speciation in Chinese Rice Wine and Associated Risk Assessment in Main Producing Provinces

et al. 2021

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Rice and rice products have been identified as significant sources of As. Concerns have been raised about the presence of As in rice wine. This study collected 79 rice wine samples from China. High-performance liquid chromatographyinductively coupled plasma mass spectrometry was used to determine total As and As species concentrations. The average concentration of total As was 14.6 μg L-1 , and the concentration of As (III) (arsenite), As (V) (arsenate), dimethylarsinic acid (DMA), and arsenobetaine (AsB) were 2.86 μg L-1 (0.970-6.08 μg L-1), 7.22 μg L-1 (2.24-22.9 μg L-1), 3.92 μg L-1 (1.58-7.82 μg L-1) and 0.620 μg L-1 (ND-0.950 μg L-1), respectively. MMA (monomethylarsonic acid) and AsC (arsenocholine) were not detected. The THQs (target hazard quotients) for chronic noncarcinogenic risks (skin lesions as the point of departure) were below 1, suggesting that the Chinese population did not encounter a significant noncarcinogenic risk. However, the mean values of MOE (margin of exposure) for lung cancer were below 100 (62.1 to 75.1) for male drinkers, indicating a potential carcinogenic risk. By comparing the As species of rice wines and the main raw material, it was found that the methylation increased DMA during fermentation. Research has shown that the concentrations of total As and As species change during grape and wine processing, 9-11 especially

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“…The iAs predominated in most of the shop-bought mushrooms, albeit with low total As concentrations. In another study, 70 species transformation of As III and As III inux and efflux across the cell membranes of fungi Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 cells were studied using a cellular lysis plus HPLC-HG-AFS and XANES analysis. The results indicated that As III can enter into fungal cells and that a portion of the As III can be exuded out of cells.…”

Section: Arsenicmentioning

confidence: 99%

Atomic Spectrometry Update: review of advances in elemental speciation

Clough

Harrington

Hill

et al. 2016

J. Anal. At. Spectrom.

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Arsenic speciation transformation and arsenite influx and efflux across the cell membrane of fungi investigated using HPLC–HG–AFS and in-situ XANES

Cited by 16 publications

References 26 publications

Arsenate toxicity and metabolism in the halotolerant microalga Dunaliella salina under various phosphate regimes

Arsenate toxicity and metabolism in the halotolerant microalga Dunaliella salina under various phosphate regimes

Concentrations of Total As and As Speciation in Chinese Rice Wine and Associated Risk Assessment in Main Producing Provinces

Atomic Spectrometry Update: review of advances in elemental speciation

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