Arsenic Status and Speciation in Chicken Heart Tissues

Abstract

SUMMARYThis study evaluated the total and main arsenic species in chicken heart tissues. The experimental study was carried out using two sets of samples. In the first one, 30-day-old chickens were exposed to sodium arsenate, using spiked drinking water. These chickens grew normally and were killed after fifty days of arsenic exposure. The second sets were edible chickens were nonexposed to sodium arsenate for a parallel study. The total arsenic and arsenic species content in the exposed samples were at least twice those in the normal edible chicken. One important aspect is the capability of the heart tissues to preconcentrate the most toxic species, arsenite, in the exposed chicken. Arsenobetaine was also detected in fat in the non exposed chicken.This study could be a way of establishing similarities and differences with humans exposed to the same As(V) species, to ascertain whether chickens can be used in arsenic metabolism evaluation and the results extrapolated to humans.

Environmental Contamination of Arsenic and its Toxicological Impact in Cardiovascular Tissues of Patients with Coronary Heart Disease

“…In general, MMA and DMA species have been considered important species in arsenic detoxification mechanisms, so it was surprising that DMA and MMA species were not detected in AU and the concentration of DMA was very low in the SAP and MAM (TABLE 7). Similar results were found in heart tissues of chickens (46). AsB is believed to have a very low toxicity and has been found in human serum (9); the presence of arsenobetaine was unexpected in this work; this organarsenical species enters human beings principally from marine foods and from agricultural products coming from In vivo methylation has long been proposed as an arsenic detoxification pathway for inorganic arsenic (22,47,48), but it has recently been shown that As(III)-MMA and As(III)-DMA, two intermediates in arsenate methylation, are more toxic to cells than As 3+ and A 5+ ions (22,47,49,50).…”

Arsenic binding proteins in cardiovascular human tissues

“…The samples were obtained from a population under study, made up of patients who have lived at least five years in the II Region of Chile. 32 Sample preparation were made on the bench of the "clean laboratory" inside a laminar flow hood (Labconco, Purifer Class II) using inert devices such as plastic and Titanium knives, agate grinding mortar, and scalpels, scissors and forceps of surgical stainless steel. After titanium clasp and fats residues removal, the tissues were rinsed with deionised water, separated as single samples and stored at -20 ºC.…”

“…After titanium clasp and fats residues removal, the tissues were rinsed with deionised water, separated as single samples and stored at -20 ºC. Specific patient's characteristics can be found in precedent work 32 and also in Table 1.…”

“…Details regarding the mineralization of tissues for total As determination and extraction of inorganic and methylated species in water: methanol extracts are given in previous papers. 32,33…”