Arsenic trioxide activates yes-associated protein by lysophosphatidic acid metabolism to selectively induce apoptosis of vascular smooth muscle cells

Yu, Hongchi; Hou, Zhe; Xiang, Maolong; Yang, Fan; Ma, Jia; Yang, Li; Ma, Xiaoyi; Zhou, Lifeng; He, Fugui; Miao, Michael; Liu, Xiaoheng; Wang, Yunbing

doi:10.1016/j.bbamcr.2022.119211

Cited by 11 publications

(6 citation statements)

References 67 publications

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“…As illustrated in Figure g, following PDT treatment, upstream factors MST1 and LATS1 exhibited decreased expression, while the expression of cotranscriptional factor YAP was increased, along with a sharp decrease in the phosphorylated YAP ( p -YAP) level in A375 cells. It is recognized that p -YAP remains in the cytoplasm, while nuclear YAP controls the ultimate transcriptional regulatory function. − Moreover, in line with the WB results, qRT-PCR analysis confirmed a significant decrease in the gene expression of MST1 and LATS1, along with upregulated YAP, in A375 cells after PDT treatment (Figure S59). It is noteworthy that immunostaining analysis results indicated that elevated YAP translocated to the nucleus of A375 cells after PDT treatment, thus triggering a strong transcriptional function on apoptotic genes (Figure h).…”

Section: Results and Discussionsupporting

confidence: 64%

Integrating Anion−π⁺ Interaction and Crowded Conformation to Develop Multifunctional NIR AIEgen for Effective Tumor Theranostics via Hippo–YAP Pathway

Yang,

Yu,

Liu

et al. 2023

ACS Nano

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The technology of aggregation-induced emission (AIE) presents a promising avenue for fluorescence imaging-guided photodynamic cancer therapy. However, existing near-infrared AIE photosensitizers (PSs) frequently encounter limitations, including tedious synthesis, poor tumor retention, and a limited understanding of the underlying molecular biology mechanism. Herein, an effective molecular design paradigm of anion−π+ interaction combined with the inherently crowded conformation that could enhance fluorescence efficacy and reactive oxygen species generation was proposed through a concise synthetic method. Mechanistically, upon photosensitization, the Hippo signaling pathway contributes to the death of melanoma cells and promotes the nuclear location of its downstream factor, yes-associated protein, which regulates the transcription and expression of apoptosis-related genes. The finding in this study would trigger the development of high-performance and versatile AIE PSs for precision cancer therapy based on a definite regulatory mechanism.

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Section: Results and Discussionsupporting

confidence: 64%

Integrating Anion−π⁺ Interaction and Crowded Conformation to Develop Multifunctional NIR AIEgen for Effective Tumor Theranostics via Hippo–YAP Pathway

Yang,

Yu,

Liu

et al. 2023

ACS Nano

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“…Among them, lysophosphatidic acid (LysoPA), which is a cell-signaling molecule closely related to the occurrence and development of vascular endothelial smooth muscle-related diseases, is the simplest structure of phospholipids. LysoPA can cause a series of vascular reactions, including platelet aggregation, smooth muscle contraction, and stimulation of smooth muscle cell proliferation [ 23 ]. Phosphatidylcholine is a phospholipid mediator with multiple biological activities and which performs important biological functions in vascular smooth muscle diseases.…”

Section: Discussionmentioning

confidence: 99%

Parsing the Q-Markers of Baoyin Jian to Treat Abnormal Uterine Bleeding by High-Throughput Chinmedomics Strategy

Ren

Liu

et al. 2023

Pharmaceuticals

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Abnormal uterine bleeding (AUB) is a common and frequently occurring disease in gynecology, seriously threatening women’s health. Baoyin Jian (BYJ) is a classical prescription for treating AUB. However, the lack of quality control standards of BYJ for AUB have limited the development and applications of BYJ. This experiment aims to explore the mechanism of action and screen the quality markers (Q-markers) of BYJ against AUB through the Chinmedomics strategy to improve the quality standards of Chinese medicine and provide scientific basis for its further development. BYJ has hemostatic effects in rats, as well as the ability to regulate the coagulation system following incomplete medical abortion. According to the results of histopathology, biochemical indexes and urine metabolomics, a total of 32 biomarkers of ABU in rats were identified, 16 of which can be significantly regulated by BYJ. Using traditional Chinese medicine (TCM) serum pharmacochemistry technology, 59 effective components were detected in vivo, of which 13 were highly correlated with efficacy, and 9 components, namely catalpol, rehmannioside D, paeoniflorin, berberine, phellodendrine, baicalin, asperosaponinVI, liquiritin, and glycyrrhizic acid, were screened out as the Q-markers of BYJ based on the “Five Principles” of Q-markers. In sum, BYJ can effectively alleviate abnormal bleeding symptoms and metabolic abnormalities in AUB rats. The study shows that Chinmedomics is an effective tool for screening Q-markers and provides scientific support for the further development and clinical use of BYJ.

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“…m6A methylation is modified by altering RNA folding and structure, which enhances mRNA export and translation [ 10 , 35 , 36 ]. m6A modification regulates cell state by regulating transcription factor expression [ 37 ]. m6A methylation also regulates mRNA stability [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis and Functional Characteristics of Differential Expression of N6-Methyladenosine Methylation Modification in the Whole Transcriptome of Rheumatoid Arthritis

Wan

Liu

Huang

et al. 2022

Mediators of Inflammation

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N6-methyladenosine (m6A) modification is the most prevalent chemical modification in eukaryotic mRNA and is associated with the development of various immune diseases. However, the role of m6A methylation in rheumatoid arthritis (RA) development is unclear. We preliminarily explored the role of m6A methylation-related mRNAs in RA for its clinical application. The discovery of m6A methylation-modifying genes in this study may provide a fresh perspective on the development of drugs for RA treatment. High-throughput sequencing combined with methylated RNA immunoprecipitation (MeRIP-seq) and RNA sequencing were used to assess whole-transcriptome m6A modifications in the synovium of patients with RA. The relationship between m6A-modified target genes and RA inflammation and macrophages was determined. The expression of the m6A-modified significant transcript-enriched inflammatory signaling pathway was assessed through animal experiments. Differentially expressed m6A genes were correlated with macrophage activation involved in immune response, vascular endothelium, MAPK signaling pathway, PI3K − Akt signaling pathway, and other inflammatory processes. Furthermore, combined analysis with m6A-seq and RNA-seq revealed 120 genes with significant changes in both m6A modification and mRNA expression. We selected the top 3 candidate mRNAs that were upregulated and downregulated simultaneously. The expression of phosphatase and tensin homolog deleted on chromosome ten (PTEN) mRNA and protein in RA patients was lower than that in healthy control (HC). SHC-binding protein 1 (SHCBP1) and neurexophilin-3 (NXPH3) mRNA expressions were increased in RA patients. The expression of M1 macrophages was increased in RA patients. RA markers are such as rheumatoid factor (RF) and peptide containing citrulline (CCP). Further animal experiments showed that the expression of synovial MAPK, PI3K, and Akt1 proteins in the RA model was increased, and the PTEN, p-PTEN protein expression was decreased. PI3K, Akt1, PTEN, and p-PTEN were correlated to RA joint inflammation. This study revealed a unique pattern of differential m6A methylation modifications in RA and concluded that m6A modification is related to the occurrence of RA synovial inflammation.

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Arsenic trioxide activates yes-associated protein by lysophosphatidic acid metabolism to selectively induce apoptosis of vascular smooth muscle cells

Cited by 11 publications

References 67 publications

Integrating Anion−π⁺ Interaction and Crowded Conformation to Develop Multifunctional NIR AIEgen for Effective Tumor Theranostics via Hippo–YAP Pathway

Integrating Anion−π⁺ Interaction and Crowded Conformation to Develop Multifunctional NIR AIEgen for Effective Tumor Theranostics via Hippo–YAP Pathway

Parsing the Q-Markers of Baoyin Jian to Treat Abnormal Uterine Bleeding by High-Throughput Chinmedomics Strategy

Comprehensive Analysis and Functional Characteristics of Differential Expression of N6-Methyladenosine Methylation Modification in the Whole Transcriptome of Rheumatoid Arthritis

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Arsenic trioxide activates yes-associated protein by lysophosphatidic acid metabolism to selectively induce apoptosis of vascular smooth muscle cells

Cited by 11 publications

References 67 publications

Integrating Anion−π+ Interaction and Crowded Conformation to Develop Multifunctional NIR AIEgen for Effective Tumor Theranostics via Hippo–YAP Pathway

Integrating Anion−π+ Interaction and Crowded Conformation to Develop Multifunctional NIR AIEgen for Effective Tumor Theranostics via Hippo–YAP Pathway

Parsing the Q-Markers of Baoyin Jian to Treat Abnormal Uterine Bleeding by High-Throughput Chinmedomics Strategy

Comprehensive Analysis and Functional Characteristics of Differential Expression of N6-Methyladenosine Methylation Modification in the Whole Transcriptome of Rheumatoid Arthritis

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Product

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Integrating Anion−π⁺ Interaction and Crowded Conformation to Develop Multifunctional NIR AIEgen for Effective Tumor Theranostics via Hippo–YAP Pathway

Integrating Anion−π⁺ Interaction and Crowded Conformation to Develop Multifunctional NIR AIEgen for Effective Tumor Theranostics via Hippo–YAP Pathway