Arsenite: the umpire of arsenate perception and responses in plants

Kandhol, Nidhi; Singh, Vijay Pratap; Herrera‐Estrella, Luis; Tran, Lam‐Son Phan; Tripathi, Durgesh Kumar

doi:10.1016/j.tplants.2022.02.005

Cited by 9 publications

(5 citation statements)

References 12 publications

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“…Nevertheless, concentrations of this metalloid remained above maximum limits permitted for several potential applications of sargassum biomass including seaweed-derived meal and feed in Europe (40 μg/g DW) (53), and for agricultural soils in different countries (15 to 50 μg/g DW) (28). In plants, both phosphate and arsenic, owing to their structural similarity, are taken up through the same mechanisms (54). In line with this, and as observed in the samples analyzed in our study, a negative correlation between P and As content was observed in sargassum samples collected at sea (55) and after stranding (56).…”

Section: Discussionmentioning

confidence: 99%

Changes in holopelagic Sargassum spp. biomass composition across an unusual year

Machado,

Marsh,

Hargreaves

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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The year 2021 marked a decade of holopelagic sargassum (morphotypes Sargassum natans I and VIII, and Sargassum fluitans III) stranding on the Caribbean and West African coasts. Beaching of millions of tons of sargassum negatively impacts coastal ecosystems, economies, and human health. Additionally, the La Soufrière volcano erupted in St. Vincent in April 2021, at the start of the sargassum season. We investigated potential monthly variations in morphotype abundance and biomass composition of sargassum harvested in Jamaica and assessed the influence of processing methods (shade-drying vs. frozen samples) and of volcanic ash exposure on biochemical and elemental components. S. fluitans III was the most abundant morphotype across the year. Limited monthly variations were observed for key brown algal components (phlorotannins, fucoxanthin, and alginate). Shade-drying did not significantly alter the contents of proteins but affected levels of phlorotannins, fucoxanthin, mannitol, and alginate. Simulation of sargassum and volcanic ash drift combined with age statistics suggested that sargassum potentially shared the surface layer with ash for ~50 d, approximately 100 d before stranding in Jamaica. Integrated elemental analysis of volcanic ash, ambient seawater, and sargassum biomass showed that algae harvested from August had accumulated P, Al, Fe, Mn, Zn, and Ni, probably from the ash, and contained less As. This ash fingerprint confirmed the geographical origin and drift timescale of sargassum. Since environmental conditions and processing methods influence biomass composition, efforts should continue to improve understanding, forecasting, monitoring, and valorizing sargassum, particularly as strandings of sargassum show no sign of abating.

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Section: Discussionmentioning

confidence: 99%

Changes in holopelagic Sargassum spp. biomass composition across an unusual year

Machado,

Marsh,

Hargreaves

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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“…The heavy metalloid contaminant arsenic (As) is prevalent and classified as a group I carcinogen, with no established safety threshold [1,2]. It easily enters the food chain through plants, posing a threat to all organisms including humans [3,4]. Arsenic pollution in farmland soil is increasingly exacerbated by ongoing industrial and agricultural activities.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Association Study of Arsenic Accumulation in Polished Rice

Dong,

Guo,

et al. 2023

Genes

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The accumulation of arsenic (As) in rice poses a significant threat to food safety and human health. Breeding rice varieties with low As accumulation is an effective strategy for mitigating the health risks associated with arsenic-contaminated rice. However, the genetic mechanisms underlying As accumulation in rice grains remain incompletely understood. We evaluated the As accumulation capacity of 313 diverse rice accessions grown in As-contaminated soils with varying As concentrations. Six rice lines with low As accumulation were identified. Additionally, a genome-wide association studies (GWAS) analysis identified 5 QTLs significantly associated with As accumulation, with qAs4 being detected in both of the experimental years. Expression analysis demonstrated that the expression of LOC_Os04g50680, which encodes an MYB transcription factor, was up-regulated in the low-As-accumulation accessions compared to the high-As-accumulation accessions after As treatment. Therefore, LOC_Os04g50680 was selected as a candidate gene for qAs4. These findings provide insights for exploiting new functional genes associated with As accumulation and facilitating the development of low-As-accumulation rice varieties through marker-assisted breeding.

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“…An example is the Arabidopsis NITROGEN LIM-ITATION ADAPTATION (NLA), which has been shown to target the Pi transporter PHT1 for degradation by 26S proteasomes (Park et al, 2014). On the contrary, SKP1-CULLIN-F-Box protein complex promotes PHR1 degradation to repress PHT1;1 expression in the presence of arsenate under low Pi conditions (Navarro et al, 2021;Kandhol et al, 2022). Recently, Guo et al (2022) reported that iron sensors, OsHRZ1 and OsHRZ2 RING-domain proteins, function as negative regulators by ubiquitinating OsPHR2 to fine-tune Pi/iron signalling and homeostasis in rice.…”

Section: Introductionmentioning

confidence: 99%

“…An example is the Arabidopsis NITROGEN LIMITATION ADAPTATION (NLA), which has been shown to target the Pi transporter PHT1 for degradation by 26S proteasomes (Park et al ., 2014). On the contrary, SKP1–CULLIN–F‐Box protein complex promotes PHR1 degradation to repress PHT1;1 expression in the presence of arsenate under low Pi conditions (Navarro et al ., 2021; Kandhol et al ., 2022). Recently, Guo et al .…”

Section: Introductionmentioning

confidence: 99%

Inositol polyphosphates‐regulated polyubiquitination of PHR1 by NLA E3 ligase during phosphate starvation response in Arabidopsis

et al. 2022

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Summary Phosphate (Pi) availability is a major factor limiting plant growth and development. The key transcription factor controlling Pi‐starvation response (PSR) is PHOSPHATE STARVATION RESPONSE 1 (PHR1) whose transcript levels do not change with changes in Pi levels. However, how PHR1 stability is regulated at the post‐translational level is relatively unexplored in Arabidopsis thaliana. Inositol polyphosphates (InsPn) are important signal molecules that promote the association of stand‐alone SPX domain proteins with PHR1 to regulate PSR. Here, we show that NITROGEN LIMITATION ADAPTATION (NLA) E3 ligase can associate with PHR1 through its conserved SPX domain and polyubiquitinate PHR1 in vitro. The association with PHR1 and its ubiquitination is enhanced by InsP6 but not by InsP5. Analysis of InsPn‐related mutants and an overexpression plant shows PHR1 levels are more stable in itpk4‐1 and vih2‐4/VIH1amiRNA but less stable in ITPK4 overexpression plants. Under Pi‐deficient conditions, nla seedlings contain high PHR1 levels, display long root hair and accumulate anthocyanin in shoots phenocopying PHR1 overexpression plants. By contrast, NLA overexpression plants phenocopy phr1 whose phenotypes are opposite to those of nla. Our results suggest NLA functions as a negative regulator of Pi response by modulating PHR1 stability and the NLA/PHR1 association depends on InsPn levels.

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Arsenite: the umpire of arsenate perception and responses in plants

Cited by 9 publications

References 12 publications

Changes in holopelagic Sargassum spp. biomass composition across an unusual year

Changes in holopelagic Sargassum spp. biomass composition across an unusual year

Genome-Wide Association Study of Arsenic Accumulation in Polished Rice

Inositol polyphosphates‐regulated polyubiquitination of PHR1 by NLA E3 ligase during phosphate starvation response in Arabidopsis

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