The common scientific metaphor linking microscopic phenomena to new or subvisible worlds in images and text has also been used to describe nanoscale phenomena. However, on closer examination, some images of the nanoscale reveal a shift away from the trope’s association with microscopic worlds and toward participatory, computer-generated worlds. This shift articulates different relations between the nanoscale and the macroscopic scale, relations that also affect how we position ourselves in relation to nanoscale phenomena and so affect the formation of nanotechnology. These different relations also create different ways to understand the nanoscale, scientific communication, and the shaping of scientific knowledge.