ART influences HIV persistence in the female reproductive tract and cervicovaginal secretions

Olesen, Rikke; Swanson, Meghan R.; Kovářová, Martina; Nochi, Tomonori; Chateau, Morgan; Honeycutt, Jenna B.; Long, Julie M.; Denton, Paul W.; Hudgens, Michael G.; Richardson, Amy; Tolstrup, Martin; Østergaard, Lars; Wahl, Angela; García, J. Víctor

doi:10.1172/jci64212

Cited by 32 publications

(53 citation statements)

References 84 publications

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“…Humanized mouse models establish latent HIV reservoirs (20–22) and show CNS infection and neuropathology (23). In NOD- scid BLT mice, HIV-infected T cells were found to form syncytia blocking the migration of infected T cells out of the lymph node suggesting cell to cell transmission (26). This was confirmed directly using NSG-BLT mice to demonstrate that trans-infection contributes to retroviral spread in vivo , providing new mechanistic means that permits retrovirus to spread among cells and should lead to new approaches for blocking virus spreading (24).…”

Section: Infectious Disease In Humanized Micementioning

confidence: 99%

“…This novel infection mechanism is particularly important in interpreting the recent clinical trial that showed anti-retroviral therapy (ART) can reduce the transmission of HIV from infected females to their partners (25). The hypothesis that this was due to suppression of HIV in the cervicovaginal secretions was tested and validated in ART-suppressed HIV-infected NSG-BLT mice (26). …”

Section: Infectious Disease In Humanized Micementioning

confidence: 99%

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Humanized Mouse Models of Clinical Disease

Walsh

Kenney

Jangalwe

et al. 2017

Annu. Rev. Pathol. Mech. Dis.

453

384

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Immunodeficient mice engrafted with functional human cells and tissues, i.e., “humanized mice”, have become increasingly important as small pre-clinical animal models for the study of human diseases. Since the description of immunodeficient mice bearing mutations in the IL2 receptor common gamma chain (IL2rgnull) in the early 2000’s, investigators have been able to engraft murine recipients with human hematopoietic stem cells that develop into functional human immune systems. These mice can also be engrafted with human tissues such as islets, liver, skin, and most solid and hematologic cancers. Humanized mice are permitting significant progress in studies of human infectious disease, cancer, regenerative medicine, graft versus host disease, allergies, and immunity. Ultimately, use of humanized mice may lead to the implementation of truly “personalized” medicine in the clinic. This review discusses recent progress in the development and use of humanized mice, and highlights their utility for the study of human diseases.

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Section: Infectious Disease In Humanized Micementioning

confidence: 99%

Section: Infectious Disease In Humanized Micementioning

confidence: 99%

Humanized Mouse Models of Clinical Disease

Walsh

Kenney

Jangalwe

et al. 2017

Annu. Rev. Pathol. Mech. Dis.

453

384

View full text Add to dashboard Cite

show abstract

“…Human immune cell reconstitution in the PB and tissues of BLT-L mice were analyzed by flow cytometry. Mononuclear cells (MNCs) were isolated from tissues collected from BLT-L mice by passage through a cell strainer and red blood cell lysis as previously described 13,14,[62][63][64] . The human lung implants, mouse lung, liver and female reproductive tract were enzymatically digested before passing tissue through a cell strainer.…”

Section: Analysis Of Human Immune Cell Reconstitutionmentioning

confidence: 99%

“…Immunohistochemical analysis. Immunohistochemical analyses were performed as previously described 14,63,64,66 . Briefly, human lung tissue and tissues collected from NSG, LoM and BLT-L mice were fixed in 4% paraformaldehyde (PFA) or 10% formalin, paraffin embedded and cut into 5 µm sections.…”

Section: Competing Interestsmentioning

confidence: 99%

Precision mouse models with expanded tropism for human pathogens

et al. 2019

Self Cite

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“…Recently, Olesen et al performed a detailed and comprehensive phenotypic characterization of the human lymphocyte subsets present in the FRT and cervicovaginal secretions (CVS) of BLT mice [68]. HIV levels and cellular dynamics in CVS during HIV infection were also analyzed together with virological suppression and cellular dynamics in the FRT and CVS during suppressive antiretroviral therapy (ART).…”

Section: The Female Reproductive Tract Of Humanized Mice Vaginal Tramentioning

confidence: 99%

Humanized mice for HIV and AIDS research

García

2016

Current Opinion in Virology

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HIV has a very limited species tropism that prevents the use of most conventional small animal models for AIDS research. The in vivo analysis of HIV/AIDS has benefited extensively from novel chimeric animal models that accurately recapitulate key aspects of the human condition. Specifically, immunodeficient mice that are systemically repopulated with human hematolymphoid cells offer a viable alternative for the study of a multitude of highly relevant aspects of HIV replication, pathogenesis, therapy, transmission, prevention, and eradication. This article summarizes some of the multiple contributions that humanized mouse models of HIV infection have made to the field of AIDS research. These models have proven to be highly informative and hold great potential for accelerating multiple aspects of HIV research in the future.

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ART influences HIV persistence in the female reproductive tract and cervicovaginal secretions

Cited by 32 publications

References 84 publications

Humanized Mouse Models of Clinical Disease

Humanized Mouse Models of Clinical Disease

Precision mouse models with expanded tropism for human pathogens

Humanized mice for HIV and AIDS research

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